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PrimePath Direct Saliva SARS-CoV-2 Detection Kit

PrimePath Direct Saliva SARS-CoV-2 Detection Kit is a research-use-only, complete kit for detecting SARS-CoV-2 directly from saliva specimens without the need for any DNA/RNA extraction. The workflow has been thoroughly formulated to ensure safe sample handling, reduce hands-on time, and deliver faster, more sensitive results.

PrimePath Direct Saliva SARS-CoV-2 Detection Kit is a research-use-only, complete kit for detecting SARS-CoV-2 directly from saliva specimens without the need for any DNA/RNA extraction. The workflow has been thoroughly formulated to ensure safe sample handling, reduce hands-on time, and deliver faster, more sensitive results.

The kit contains all components required to collect the saliva specimens and run the RT-qPCR detection assay, including our high-performance One-Step PrimeScript III RT-qPCR Kit (Cat. # RR600A). This assay is based on primers and probes designed and published by the CDC.

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Cat. # Product Size License Quantity Details
638340 PrimePath™ Direct Saliva SARS-CoV-2 Detection Kit 200 Rxns USD $1355.00

Complete end-to-end kit for collecting samples and running an RT-qPCR assay to detect SARS-CoV-2 in saliva without needing any DNA/RNA extraction or additional heat-lysis steps.

NOTE: Cat. No. 638340 contains Cat. Nos. 638341–3 and RR600A. Customers have the option of purchasing these kit components separately, but this is not required with the purchase of Cat. No. 638340.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data
638341 PrimePath™ SARS-CoV-2 Assay Mix 200 Rxns USD $59.00

PrimePath SARS-CoV-2 Assay Mix is a 20X mixture of primers and probes targeting the N1 (FAM) and N2 (HEX) regions of the nucleocapsid gene of the SARS-CoV-2 genome and the human RNaseP gene (Cy5). The primer and probe designs are based on those published by the CDC. Please refer to the CDC's Research Use Only 2019-Novel Coronavirus (2019-nCoV) Real-time RT-PCR Primers and Probes for more information.

NOTE: Cat. No. 638341 is included with Cat. No. 638340. It can also be purchased separately.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data
638342 PrimePath™ SARS-CoV-2 Positive Control Each USD $990.00

PrimePath SARS-CoV-2 Positive Control is an in vitro-transcribed RNA control for use with SARS-CoV-2 qPCR detection assays. It comprises the entire nucleocapsid gene, including N1 and N2 regions, of the SARS-CoV-2 genome.

NOTE: Cat. No. 638342 is included with Cat. No. 638340. It can also be purchased separately.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data
638343 PrimePath™ Saliva Collection Kit 200 Preps USD $317.00

The PrimePath Saliva Collection Kit contains collection sticks and a viral inactivation buffer that is used both for storage and processing samples. At one end of each collection stick, there are channels for collecting liquid such as saliva. When mixed with the collected saliva sample, the buffer will inactivate any SARS-CoV-2 particles that are present. This process makes the saliva compatible with the RT-qPCR step in the PrimePath Saliva Direct SARS-CoV-2 Detection Kit.

NOTE: Cat. No. 638343 is included with Cat. No. 638340. It can also be purchased separately.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data
RR600A One Step PrimeScript™ III RT-PCR Kit 200 Rxns USD $386.00

One Step PrimeScript III RT-qPCR Mix is a dedicated reagent for one-step real-time, probe-based RT-qPCR (using the 5’ nuclease method). This 2X premix does not freeze at its storage temperature of –20℃, so a reaction can be started simply by adding the template sample, primer, and a probe for detecting the desired target. The quick and simple protocol allows the reverse transcription and qPCR reactions to be performed in the same tube. The reverse transcription reaction uses the novel PrimeScript III RTase, which displays increased heat tolerance (up to 55℃) while maintaining the specificity and extensibility of PrimeScript RTase. This allows cDNA synthesis from RNA with a more complex secondary structure. After cDNA synthesis, TaKaRa Taq HS performs highly specific and efficient PCR amplification, while the fluorescence emitted by the probe is detected in real-time. One Step PrimeScript III RT-qPCR Mix is also highly resistant to a wide variety of inhibitory substances in blood and soil, allowing stable one-step real-time RT-qPCR to be performed on a wide range of samples. This product can be used for various applications such as gene expression, RNA virus detection, etc.

Cat. # RR600A contains sufficient reagent for performing 200 reactions in a volume of 25 µl per reaction.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Overview

  • Complete kit—all components needed for the user to perform SARS-CoV-2 detection are included (except the qPCR instrument). The kit includes our high-performance One Step PrimeScript III RT-PCR Kit for a simple setup. The in vitro-transcribed (IVT) PrimePath SARS-CoV-2 Positive Control is validated using ddPCR and comprises exact copies of the target RNA sought by the CDC-designed primers and probes in this assay.
  • Easier, safer sample collection and handling—PrimePath Saliva Collection Kit provides unique collection sticks for easy capture of saliva samples. Additionally, the kit comes with a buffer that inactivates SARS-CoV-2 once the saliva sample is dissolved, making it safer for handling and transportation.
  • Faster results—no DNA/RNA extraction is necessary. A total qPCR assay detecting SARS-CoV-2 can be completed in <30 minutes.

More Information

Applications

  • Detection of SARS-CoV-2 in unpurified saliva samples

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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