Advantage HF 2 PCR Kit

The Advantage HF 2 PCR Kit is a high-performance PCR system optimized for error-free amplification of genomic and cDNA targets up to 3.5 kb. The kit contains the hot-start Advantage 2 polymerase blend of Titanium Taq DNA Polymerase and a small amount of proofreading enzyme. This blend provides high sensitivity when amplifying a wide range of DNA targets. The kit combines this enzyme blend with a proprietary mix of dNTPs and a specially formulated buffer that work together to achieve 30-fold higher fidelity than that seen with wild-type Taq DNA polymerase.

Cat. #	Product	Size	Price
639124	Advantage® HF 2 PCR Kit	10 Rxns	USD $191.00
This kit pairs Takara's hot-start Advantage 2 Polymerase Mix with a proprietray dNTP mix to specially amplify fragments up to 3.5 kb with exceptionally high fidelity (30-fold higher than wild-type Taq). Enough reagents are supplied for 10 PCR amplifications of 50 μl each. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Amplification with the Advantage HF 2 PCR Kit Amplification with the Advantage HF 2 PCR Kit. Panel A. Fidelity (accuracy) of Taq, Advantage 2 Polymerase Mix, and Advantage HF 2 PCR Kit were measured as previously described (1, 2) in a genetic screen. Fidelity of the Advantage enzyme mixes was normalized to Taq, which produced 0.66 errors per 1,000 bp of amplified sequence after 25 PCR cycles. Advantage 2 produced 0.24 errors per 1,000 bp and Advantage HF 2 produced 0.023 errors per 1,000 bp. Panel B. Amplification of a wide size range of cDNA and genomic fragments. Lanes 1–3: amplification of several fragments (0.5–1.8 kb) of the human cardiac beta-myosin chain from human genomic DNA. ;Lanes 4–6: amplification of several size fragments (1.0–3.5 kb) of the BPTI gene from calf thymus DNA. Lanes 7–8: amplification from Marathon-Ready Human Placenta cDNA (Cat. No. 639311). Lane 7: 1.2-kb beta-actin fragment. Lane 8: 1.3-kb TFR fragment. Lane M: 1-kb DNA size marker. Back 639124: Advantage HF 2 PCR Kit
639123	Advantage® HF 2 PCR Kit	100 Rxns	USD $507.00
This kit pairs Takara's hot-start Advantage 2 Polymerase Mix with a proprietray dNTP mix to specially amplify fragments up to 3.5 kb with exceptionally high fidelity (30-fold higher than wild-type Taq). Enough reagents are supplied for 100 PCR amplifications of 50 μl each. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Amplification with the Advantage HF 2 PCR Kit Amplification with the Advantage HF 2 PCR Kit. Panel A. Fidelity (accuracy) of Taq, Advantage 2 Polymerase Mix, and Advantage HF 2 PCR Kit were measured as previously described (1, 2) in a genetic screen. Fidelity of the Advantage enzyme mixes was normalized to Taq, which produced 0.66 errors per 1,000 bp of amplified sequence after 25 PCR cycles. Advantage 2 produced 0.24 errors per 1,000 bp and Advantage HF 2 produced 0.023 errors per 1,000 bp. Panel B. Amplification of a wide size range of cDNA and genomic fragments. Lanes 1–3: amplification of several fragments (0.5–1.8 kb) of the human cardiac beta-myosin chain from human genomic DNA. ;Lanes 4–6: amplification of several size fragments (1.0–3.5 kb) of the BPTI gene from calf thymus DNA. Lanes 7–8: amplification from Marathon-Ready Human Placenta cDNA (Cat. No. 639311). Lane 7: 1.2-kb beta-actin fragment. Lane 8: 1.3-kb TFR fragment. Lane M: 1-kb DNA size marker. Back 639123: Advantage HF 2 PCR Kit

Overview

Provides virtually error-free amplification—nearly 30-fold higher fidelity than wild-type Taq
Produces exceptionally high yields without sacrificing fidelity
Pre-optimized kit components eliminate the need to optimize reaction conditions
Generates PCR products (up to 5 kb) that can be cloned into any TA-based vector

More Information

Applications

Cloning/TA-cloning
Mutation analysis
Long and accurate PCR
High-fidelity PCR amplification

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Find answers to your PCR questions

Primer design

Frequently asked questions about primer design for successful PCR.

Optimization

Frequently asked questions about PCR optimization.

Troubleshooting

Frequently asked questions about troubleshooting your PCR problems.

Applications and conditions

Frequently asked questions about general and specific applications for PCR and which polymerases to choose.

Shipping, storage, and handling

Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.

Avoid DNA contamination in PCR

There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2025 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Advantage HF 2 PCR Kit

Notice to purchaser

Amplification with the Advantage HF 2 PCR Kit

639124: Advantage HF 2 PCR Kit