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Single-cell NGS automation

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Single-cell full-length transcriptome analysis on the ICELL8 system powered by SMART-Seq technology

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Full-length sequence information allows many types of analysis—such as differential gene expression, gene fusion, and alternative splicing—with high sensitivity. Our high-throughput solution leverages SMART-Seq chemistry for sensitive, full-length transcriptome profiling of 1,000–2,000 cells isolated on the ICELL8 Single-Cell System.

Please refer to the table in the "Components" section below for ordering information.

Full-length sequence information allows many types of analysis—such as differential gene expression, gene fusion, and alternative splicing—with high sensitivity. Our high-throughput solution leverages SMART-Seq chemistry for sensitive, full-length transcriptome profiling of 1,000–2,000 cells isolated on the ICELL8 Single-Cell System. The workflow incorporates Illumina-specific adapter sequences during cDNA amplification on an ICELL8 chip for the generation of indexed libraries that are ready for sequencing on Illumina platforms.

Please refer to the table in the "Components" section below for ordering information.

Single cells isolated with the ICELL8 Single-Cell System (Cat. # 640000) are dispensed into a 5,184-nanowell, blank ICELL8 250v Chip (Cat. # 640183). Following identification of single-cell-bearing wells using CellSelect Software, SMART RT-PCR reagents are then dispensed to the wells of samples chosen for further processing. The chip is then transferred to the ICELL8 Chip Cycler, which is programmed to perform one-step RT-PCR for first-strand synthesis and amplification of cDNA in a single run. The full-length cDNA is tagmented by TDE1 (Illumina Tagment DNA Enzyme), and then the tagmented cDNA is amplified using forward and reverse indexing primers (Cat. # 640205 and/or 640218). The resulting library is extracted, purified, and amplified to yield the final sequencing-ready library.

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Cat. # Product Size License Quantity Details
640219 SMART-Seq® ICELL8® Application Kit – 1 Chip 1 Rxn USD $2542.00

SMART-Seq ICELL8 Application Kit – 1 Chip contains the necessary chip, reagents, and consumables required to run one experiment of single-cell, full-length transcriptome analysis on the ICELL8 Single-Cell System (Cat. No. 640000). Additional consumables such as MSND 384-Well Source Plates and Seals (Cat. Nos. 640018 or 640037) can be purchased separately .

The protocols for performing SMART-Seq chemistry on ICELL8 systems leverage SMART technology for full-length cDNA synthesis and Nextera® technology for Illumina library preparation with dual 72 x 72 indexes (Nextera Tagment DNA Enzyme not included). The protocols generate indexed libraries that are ready for sequencing on Illumina platforms.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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640220 SMART-Seq® ICELL8® Application Kit – 3 Chip 3 Rxns USD $7401.00

SMART-Seq ICELL8 Application Kit – 3 Chip contains the necessary chips, reagents, and consumables required to run three experiments of single-cell, full-length transcriptome analysis on the ICELL8 Single-Cell System (Cat. No. 640000). Additional consumables such as MSND 384-Well Source Plates and Seals (Cat. No. 640018, 640037, 640192) can be purchased separately. The SMART-Seq ICELL8 Indexing Primer Set – B (Cat. No. 640218) with additional indexes can be used with separate chip, for multiplexing two-chip experiments.

The protocols for performing SMART-Seq chemistry on ICELL8 systems leverage SMART technology for full-length cDNA synthesis and Nextera technology for Illumina library preparation with dual 72 x 72 indexes (Nextera Tagment DNA Enzyme not included). The protocols generate indexed libraries that are ready for sequencing on Illumina platforms.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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640221 SMART-Seq® ICELL8® Application Kit – 5 Chip 5 Rxns USD $12090.00

SMART-Seq ICELL8 Application Kit – 5 Chip contains the necessary chips, reagents, and consumables required to run five experiments of single-cell, full-length transcriptome analysis on the ICELL8 Single-Cell System (Cat. No. 640000). Additional consumables such as MSND 384-Well Source Plates and Seals (Cat. No. 640018, 640037, 640192) can be purchased separately. The SMART-Seq ICELL8 Indexing Primer Set – B (Cat. No. 640218) with additional indexes can be used with separate chip, for multiplexing two-chip experiments.

The protocols for performing SMART-Seq chemistry on ICELL8 systems leverage SMART technology for full-length cDNA synthesis and Nextera technology for Illumina library preparation with dual 72 x 72 indexes (Nextera Tagment DNA Enzyme not included). The protocols generate indexed libraries that are ready for sequencing on Illumina platforms.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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640018 MSND 384-Well Source Plate and Seals (430-000025) 20/Pack USD $450.00

20 Pack of MSND 384-Well Source Plate and Seals. Formerly 430-000025.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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640037 MSND 384-well Source Plate and Seals (430-000258) 120/Pack USD $1665.00

120 pack of MSND 384-well Source Plate and Seals. Formerly 430-000258.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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640192 ICELL8® 384-Well Source Plate and Seal Each USD $175.00

The ICELL8 384-Well Source Plate and Seal set is a component of the ICELL8 cx Single-Cell System. It includes NUNC plates and seals for covering the plates, which are utilized for sample and reagent dispense into SmartChip plates for related NGS applications.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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640202 SMART-Seq® ICELL8® Reagent Kit 1 Chip USD $499.00

SMART-Seq ICELL8 reagent kits, available for purchase separately, combine with indexing primer sets (SMART-Seq ICELL8 Indexing Primer Set – A [Cat. No. 640205] and/or SMART-Seq ICELL8 Indexing Primer Set - B [Cat. No. 640218]) and ICELL8 250v chips (Cat. No. 640183) or ICELL8 350v chips (Cat. No. 640019) for use with the SMART-Seq ICELL8 or ICELL8 cx application protocols, respectively. These protocols enable users to analyze full-length transcripts using ICELL8 single-cell systems. This kit (Cat. No. 640202) contains reagents sufficient for running one chip.

The protocols for performing SMART-Seq chemistry on ICELL8 systems leverage SMART technology for full-length cDNA synthesis and Nextera® technology for Illumina library preparation with dual 72 x 72 indexes (Nextera Tagment DNA Enzyme not included). The protocols generate indexed libraries that are ready for sequencing on Illumina platforms.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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640203 SMART-Seq® ICELL8® Reagent Kit 3 Chips USD $1272.00

SMART-Seq ICELL8 reagent kits, available for purchase separately, combine with indexing primer sets (SMART-Seq ICELL8 Indexing Primer Set – A [Cat. No. 640205] and/or SMART-Seq ICELL8 Indexing Primer Set - B [Cat. No. 640218]) and ICELL8 250v chips (Cat. No. 640183) or ICELL8 350v chips (Cat. No. 640019) for use with the SMART-Seq ICELL8 or ICELL8 cx application protocols, respectively. These protocols enable users to analyze full-length transcripts using ICELL8 single-cell systems. This kit (Cat. No. 640203) contains reagents sufficient for running three chips.

The protocols for performing SMART-Seq chemistry on ICELL8 systems leverage SMART technology for full-length cDNA synthesis and Nextera® technology for Illumina library preparation with dual 72 x 72 indexes (Nextera Tagment DNA Enzyme not included). The protocols generate indexed libraries that are ready for sequencing on Illumina platforms.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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640204 SMART-Seq® ICELL8® Reagent Kit 5 Chips USD $1875.00

SMART-Seq ICELL8 reagent kits, available for purchase separately, combine with indexing primer sets (SMART-Seq ICELL8 Indexing Primer Set – A [Cat. No. 640205] and/or SMART-Seq ICELL8 Indexing Primer Set – B [Cat. No. 640218]) and ICELL8 250v chips (Cat. No. 640183) or ICELL8 350v chips (Cat. No. 640019) for use with the SMART-Seq ICELL8 cx or ICELL8 application protocols, respectively. These protocols enable users to analyze full-length transcripts using ICELL8 single-cell systems. This kit (Cat. No. 640204) contains reagents sufficient for running five chips.

The protocols for performing SMART-Seq chemistry on ICELL8 systems leverage SMART technology for full-length cDNA synthesis and Nextera® technology for Illumina library preparation with dual 72 x 72 indexes (Nextera Tagment DNA Enzyme not included). The protocols generate indexed libraries that are ready for sequencing on Illumina platforms.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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640205 SMART-Seq® ICELL8® Indexing Primer Set – A 1 Chip USD $875.00

The SMART-Seq ICELL8 Indexing Primer Set – A enables users to analyze full-length transcripts using ICELL8 single-cell systems. The SMART-Seq ICELL8 Indexing Primer Set – A contains sufficient indexes to process samples on one ICELL8 250v Chip or one ICELL8 350v Chip with one SMART-Seq ICELL8 Reagent Kit (1-chip size).

The protocols for performing SMART-Seq chemistry on ICELL8 systems leverage SMART technology for full-length cDNA synthesis and Nextera® technology for Illumina library preparation with dual 72 x 72 indexes (Nextera Tagment DNA Enzyme not included). The protocols generate indexed libraries that are ready for sequencing on Illumina platforms.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data
640218 SMART-Seq® ICELL8® Indexing Primer Set - B 1 Chip USD $875.00

The SMART-Seq ICELL8 Indexing Primer Set – B enables users to analyze full-length transcripts using the ICELL8 cx System or the ICELL8 Single-Cell System. It contains sufficient indexes to process samples on one chip with one SMART-Seq ICELL8 Reagent Kit (1-chip size). The indexing primers from this set and SMART-Seq ICELL8 Indexing Primer Set – A (Cat. No. 640205) can be used on different ICELL8 chips for SMART-Seq application. The libraries can be pooled for sequencing.

This primer set contains two boxes in total. Each box contains a 384-well plate prealiquoted with indexes (20 µl per well). Box 1 includes Plate 1 containing 72 forward indexes (1-72), and Box 2 includes Plate 2 containing 72 reverse indexes (73-144).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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640183 ICELL8® 250v Chip 1 Chip USD $698.00

The ICELL8 250v Chip is a 250-nl volume, V-bottom chip that enables users to dispense and image cells using the ICELL8 Single-Cell System. All wells in the 5,184-nanowell chip are unprinted and have a maximum working volume of 250 nl and a depth of 1.600 mm. Cells of choice are diluted, as per the appropriate application-specific ICELL8 user manual, and then dispensed and/or imaged on the ICELL8 system. CellSelect Software is used to image and detect single-cell-bearing wells.

The ICELL8 250v Chip is used in the SMART-Seq ICELL8 application kit protocol along with the SMART-Seq ICELL8 Reagent Kit, which enables users to analyze full-length transcripts using the ICELL8 Single-Cell System.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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640206 ICELL8® Loading Kit – B Each USD $95.00

The ICELL8 Loading Kit – B contains blotting paper, optical film, and sealing film to be used for processing ICELL8 chips in workflows with multiple incubation steps.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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640048 ICELL8® Collection Kit 1 Pkg USD $25.00

The ICELL8 Collection Kit consists of a fixture, tube, and film that allow users to spin down the contents of an ICELL8 chip to collect the contents of processed chips (e.g., extracted cDNA) into a tube.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Required Products

Cat. # Product Size Price License Quantity Details
640000 ICELL8® Single-Cell System Each Inquire for Quotation *

The ICELL8 Single-Cell System delivers unparalleled confidence and control for single-cell analysis by enabling precise cell isolation, cell selection, and high sample throughput. The system includes an ICELL8 MultiSample NanoDispenser, a complete imaging system—including microscope, computer, and software—a thermal cycler that accomodates ICELL8 chips, and additional accessories.

Documents Components Image Data

SMART-Seq ICELL8 application kit protocol SMART-Seq ICELL8 Application Kit User Manual
Technical note Discover more with automated, full-length scRNA-seq
NGS logo Poster: full-length scRNA-seq on ICELL8 system

Overview

  • High-throughput single-cell analysis—generate sequencing data from >1,000 single cells in parallel
  • Multiplex for higher throughput—use SMART-Seq ICELL8 indexing primer sets A and B on two chips, and then pool the samples to allow sequencing of >2,000 cells simultaneously
  • Sample flexibility—analyze cells from up to eight different samples in a single experiment
  • Reduced sample handling—minimize variability with automated cell isolation and selection, and sample pooling prior to sequencing-ready library construction
  • Illumina sequencing libraries—generate libraries suitable for sequencing on all Illumina platforms

More Information

Applications

  • High-throughput, full-length single cell RNA-seq library preparation for Illumina platforms

Components

The following Takara Bio items are required to complete the workflow. Please refer to the SMART-Seq ICELL8 Application Kit User Manual for a complete list of materials required.

The following kits contain the necessary chip(s), reagents, and consumables required to run one, three, or five experiments (respectively) of single-cell, full-length transcriptome analysis.

ICELL8 Single-Cell System (Cat. # 640000)
Cat. #ProductNumber of experiments per kit
640219 SMART-Seq ICELL8 Application Kit – 1 Chip1 1
640220 SMART-Seq ICELL8 Application Kit – 3 Chip1 3
640221 SMART-Seq ICELL8 Application Kit – 5 Chip1 5
SMART-Seq ICELL8 application kit components
Cat. #Component2640219
(1 Chip)		640220
(3 Chips)		640221
(5 Chips)
640183 ICELL8 250v Chip 1 3 5
640212 ICELL8 Collection Kit – L 1 3 5
640206 ICELL8 Loading Kit – B 1 3 5
640202 SMART-Seq ICELL8 Reagent Kit 1 — —
640203 SMART-Seq ICELL8 Reagent Kit — 1 —
640204 SMART-Seq ICELL8 Reagent Kit — — 1
640205 SMART-Seq ICELL8 Indexing Primer Set – A 1 2 3
640218 SMART-Seq ICELL8 Indexing Primer Set – B — 1 2

Notes:

  1. The application kits contain Cat. # 640212 (ICELL8 Collection Kit – L), which must be used with the ICELL8 Extraction Fixture – L (Cat. # 640217, purchased separately). If needed, please either log in to your research or business account and use 640217 to quick order as a one-time purchase or contact Customer Service to order one.
  2. Components can also be purchased separately.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

ICELL8 system

Improve detection of fusions, SNPs, and alternative splicing with the ICELL8 system

We adapted our trusted SMART-Seq chemistry on the ICELL8 system for improved full-length scRNA-seq, so you can:

  • Fully automate the processing of >1,000 individual cells into sequencing-ready libraries
  • Achieve uniform coverage from 5'→3'
  • Improve detection of gene fusions, SNPs, and splice variants

Read the technical note to see how our method compares to a 3'DE- and droplet-based method.

Read more

Single-cell identification with CellSelect Software

See how the ICELL8 platform's Imaging System and CellSelect Software allow for high-fidelity identification of individual cells.

Learn more

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