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Tet-On 3G tetracycline-inducible expression systems

Tet-On 3G inducible gene expression systems

Tet-On 3G tetracycline-inducible gene expression systems are the third generation of the most powerful, versatile, and widely cited inducible mammalian expression systems available. The Tet-On 3G system offers a significant improvement over Tet-On and Tet-On Advanced systems with significantly reduced basal expression and increased sensitivity to doxycycline, a tetracycline analogue.

Tet-On 3G tetracycline-inducible gene expression systems are the third generation of the most powerful, versatile, and widely cited inducible mammalian expression systems available. The Tet-On 3G system offers a significant improvement over Tet-On and Tet-On Advanced systems with significantly reduced basal expression and increased sensitivity to doxycycline, a tetracycline analogue.

Learn more about the high sensitivity of the transactivator, and the low background from the tight promoter in the Tet system learning center.

Choice of Tet-On 3G vector formats

There is a range of available Tet-On 3G vector formats:

  • Tet-One systems (listed elsewhere) provide a complete inducible expression system on a single vector.
  • The Tet-On 3G Inducible Expression System (EF1alpha Version), our EF1-alpha promoter version of the Tet-On 3G system, provides consistent long-term expression of the Tet-On 3G transactivator, even in cell types known for their tendency to silence a CMV promoter over time, such as hematopoietic cells and stem cells.
  • Bidirectional promoter versions of the system allow you to induce expression of two genes from the same promoter—either two transgenes of your choosing, or a single transgene and a fluorescent protein. If your cells turn red or green after adding Dox, this confirms that your gene has been turned on and that you have selected a high-performing inducible clone.
  • Using IRES technology, we've married very tight gene expression control to a bright red fluorescent protein (mCherry) in the Tet-On 3G Inducible Expression System (with mCherry), and a bright green fluorescent protein (ZsGreen1) in the Tet-On 3G Inducible Expression System (with ZsGreen1). 
  • Retroviral and lentiviral formats include the PTRE3GV promoter, which is optimized for use in retroviral and lentiviral vectors. They are supplied with complete viral packaging systems.

Use tetracycline-approved serum for optimal results

With the greatly increased sensitivity of the Tet-On 3G system, it is more important than ever that the serum you use for your studies is guaranteed to be tetracycline-free. Only Takara Bio performs actual inducibility tests on a sensitive Tet-inducible cell line in order to provide an absolute guarantee that our serum will not affect basal expression in your Tet-On 3G experiments. Each Tet-On 3G system is supplied with 50 ml of our premium Tet System Approved FBS.

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Cat. # Product Size Price License Quantity Details
631164 Tet-On® 3G Inducible Expression System (with ZsGreen1) Each Inquire for Quotation *

The Tet-On 3G Inducible Expression System is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. The Tet-On 3G Vector Set (with ZsGreen1) allows the simultaneous expression of a gene of interest and a green fluorescent protein marker. The system includes a vector set, our highly efficient transfection reagent, Xfect, and Tet System Approved FBS.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Required Products

Cat. # Product Size Price License Quantity Details
631101 Tet System Approved FBS, US-Sourced 500 mL USD $971.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

Documents Components Image Data
631106 Tet System Approved FBS 500 mL USD $933.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

Documents Components Image Data
631165 Tet-On® 3G Inducible Expression System (with mCherry) Each Inquire for Quotation *

The Tet-On 3G Inducible Expression System (with mCherry) is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. It allows the simultaneous expression of a gene of interest and a red fluorescent protein marker. The system includes all necessary vectors, our highly efficient transfection reagent, Xfect, and Tet System Approved FBS.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Required Products

Cat. # Product Size Price License Quantity Details
631101 Tet System Approved FBS, US-Sourced 500 mL USD $971.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

Documents Components Image Data
631106 Tet System Approved FBS 500 mL USD $933.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

Documents Components Image Data
631168 Tet-On® 3G Inducible Expression System Each Inquire for Quotation *

The Tet-On 3G Inducible Expression System is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. The system includes all necessary vectors, our highly efficient transfection reagent, Xfect, and Tet System Approved FBS.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Required Products

Cat. # Product Size Price License Quantity Details
631101 Tet System Approved FBS, US-Sourced 500 mL USD $971.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

Documents Components Image Data
631106 Tet System Approved FBS 500 mL USD $933.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

Documents Components Image Data
631337 Tet-On® 3G Bidirectional Inducible Expression System Each Inquire for Quotation *

The Tet-On 3G Bidirectional Inducible Expression System is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. This system allows the simultaneous expression of two genes of interest from a bidirectional, Tet-responsive promoter that binds the Tet-On 3G transactivator protein in the presence of doxycycline. It includes the pTRE3G-BI Vector Set, consisting of a bidirectional vector into which both genes of interest can be cloned and simultaneously expressed, a bidirectional control vector that contains the luciferase gene in one of the two possible cloning sites, and two linear selection markers for hygromycin and puromycin resistance—and the pCMV-Tet3G Vector, which constitutively expresses the Tet-On 3G transactivator protein from a CMV promoter. This system also includes our highly efficient Xfect Transfection Reagent and Tet System Approved FBS.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Required Products

Cat. # Product Size Price License Quantity Details
631101 Tet System Approved FBS, US-Sourced 500 mL USD $971.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

Documents Components Image Data
631106 Tet System Approved FBS 500 mL USD $933.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

Documents Components Image Data
631338 Tet-On® 3G Bidirectional Inducible Expression System (with mCherry) Each Inquire for Quotation *

The Tet-On 3G Inducible Expression System (with mCherry) is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. This system allows the simultaneous expression of a gene of interest and a red fluorescent protein (mCherry) marker from a bidirectional, Tet-responsive promoter that binds the Tet-On 3G transactivator protein in the presence of doxycycline. It includes the pTRE3G-BI-mCherry Vector Set, consisting of a bidirectional vector into which the gene of interest can be cloned and simultaneously expressed with the mCherry marker, a bidirectional control vector that contains the luciferase gene in one of the two possible cloning sites, and two linear selection markers for hygromycin and puromycin resistance—and the pCMV-Tet3G Vector, which constitutively expresses the Tet-On 3G transactivator protein from a CMV promoter. This system also includes our highly efficient Xfect Transfection Reagent and Tet System Approved FBS.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Required Products

Cat. # Product Size Price License Quantity Details
631101 Tet System Approved FBS, US-Sourced 500 mL USD $971.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

Documents Components Image Data
631106 Tet System Approved FBS 500 mL USD $933.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

Documents Components Image Data
631339 Tet-On® 3G Bidirectional Inducible Expression System (with ZsGreen1) Each Inquire for Quotation *

The Tet-On 3G Inducible Expression System (with ZsGreen1) is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. This system allows the simultaneous expression of a gene of interest and a green fluorescent protein (ZsGreen1) marker from a bidirectional, Tet-responsive promoter that binds the Tet-On 3G transactivator protein in the presence of doxycycline. It includes the pTRE3G-BI-ZsGreen1 Vector Set, consisting of a bidirectional vector into which the gene of interest can be cloned and simultaneously expressed with the ZsGreen1 marker, a bidirectional control vector that contains the luciferase gene in one of the two possible cloning sites, and two linear selection markers for hygromycin and puromycin resistance—and the pCMV-Tet3G Vector, which constitutively expresses the Tet-On 3G transactivator protein from a CMV promoter. This system also includes our highly efficient Xfect Transfection Reagent and Tet System Approved FBS.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Required Products

Cat. # Product Size Price License Quantity Details
631101 Tet System Approved FBS, US-Sourced 500 mL USD $971.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

Documents Components Image Data
631106 Tet System Approved FBS 500 mL USD $933.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

Documents Components Image Data

*You must be logged in to a Purchasing Account in order to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@takarabio.com.

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Overview

More Information

Applications

  • Inducible expression in mammalian cells
  • Inducible expression in stem cells and hematopoietic cells (EF1-alpha promoter version)
  • Co-inducible expression of two genes of interest (bicistronic version)
  • Co-express a fluorescent protein (mCherry and ZsGreen1 versions)

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Tet-One tetracycline-inducible expression system, technology overview

Tighly controlled expression system that is as easy to use as any constitutive system

Until now, the best inducible mammalian expression systems have all required a two-vector format. Tet-One inducible expression systems are the only commercially available single-vector systems that can deliver a >10,000-fold difference in expression levels between induced and uninduced states.

Tet-One technology overview All inducible systems

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