Find the right qPCR tool

CellAmp Direct RNA Prep Kit RT-PCR (qPCR) is for preparation of templates used in one-step and two-step real-time RT-PCR utilizing a simple method of extraction directly from cultured cells in 96 well-plates or others. It is possible to prepare templates for real-time PCR from cultured cells in approximately 10 minutes. Use of this kit with a one-step real-time RT-PCR kit (i.e. One Step TB Green Ex Taq qRT-PCR Kit) gene expression analysis can be performed in approximately 2 hours. Templates can be prepared directly from a small quantity of cells using this kit, facilitating gene expression analysis by sensitive real-time RT-PCR. Excellent for detection of rare transcripts.

Back

3732: CellAmp Direct RNA Prep Kit for RT-PCR (Real Time)

The CellAmp Direct RNA Prep Kit for qRT-PCR and Protein Analysis allows simple, direct extraction directly from cultured cells to prepare templates used for one-step and two-step qRT-PCR. The kit can be used for cells cultured in 96-well plates and is compatible with high-throughput studies. Preparation of templates for real-time PCR from cultured cells takes approximately 10 minutes and complete gene expression analysis (via One Step or Two Step qRT-PCR) is performed in approximately 2 hours. When used in combination with a reverse transcription kit, cDNA templates for real-time PCR can be synthesized in about 30 minutes, providing efficient high-throughput analysis. Templates can be prepared directly from small quantities of cells for analysis by high sensitivity qRT-PCR. Genomic DNA can be easily and efficiently removed using this kit. Excellent for detection of rare transcripts.

Back

3733: CellAmp RT-PCR for Direct Prep Kit (Real Time) & Protein Analysis

This product is used to dilute template DNA or RNA when preparing serial dilutions of the standard for establishing the standard curve in real time PCR.

Back

9160: EASY Dilution (for Real Time PCR)

A reference gene is often used to normalize the total amount of input nucleic acid for gene expression analysis. The level of target gene expression is compared to the reference gene, a method called relative quantitation. Housekeeping genes are commonly used as the reference, but it may be difficult to identify a gene with stable expression in some experimental systems. In such cases, adding an external standard RNA to the reaction to serve as a reference may be useful for relative quantification.

Back

3789: External Standard Kit (Lambda PolyA) for qPCR

Mixture of total RNAs from a collection of adult mouse tissues, chosen to represent a broad range of expressed genes. Both male and female animals are represented. Universal Reference Total RNA serves as a standard for comparing gene expression data and is useful as an internal control for microarray expression profiling experiments.

Total RNA was isolated by a modified guanidinium thiocyanate method.

Back

Clontech Rat Universal Reference Total RNA demonstrates more than 90% gene coverage

Clontech Rat Universal Reference Total RNA demonstrates more than 90% gene coverage. Cy3 labeled probes were generated using our Rat Reference Total RNA (Row A), another vendor’s reference total RNA (Row B), a random RNA mixture (Row C), and an individual RNA sample (Row D). Probes were hybridized to a glass rat array containing 3,766 long oligonucleotides. Expression results were analyzed using GeneSpring software (version 5.0) to cluster genes according to their expression patterns. Varying colors reflect the ratio of the intensity of any gene on each array to its median intensity across all arrays. The red and blue colors reflect high and low ratios, respectively. Our results indicate that the Reference Total RNA has more than 90% gene coverage with even distribution and outperforms another vendor’s universal RNA mixture.

Back

636657: Mouse Universal Reference Total RNA

cDNAs prepared from a mixture of total RNAs collected from adult normal human tissues. This cDNA mixture has been chosen to represent a broad range of expressed genes.

qPCR Human Reference cDNA serves as a standard for accurate and reproducible comparison of gene expression data using qPCR (quantitative PCR) as well as a positive control template for validation of gene primer design. Enough cDNA is supplied for either 25 or 100 reactions requiring 2 μl of sample per reaction; the number of reactions is based on a 50 μl reaction volume.

The sample was oligo(dT) primed.

Back

High-, medium- and low-abundance gene targets are easily detected in qPCR Human Reference cDNA

High-, medium- and low-abundance gene targets are easily detected in qPCR Human Reference cDNA. Oligo dT-primed (Panels A–C) and random oligo-primed (Panels D–F) qPCR Human Reference cDNA samples were each serially diluted fivefold such that the final quantities of cDNA template in the qPCR reactions were 20 ng, 4 ng, 800 pg, 160 pg, 32 pg,and 6.4 pg. Data were obtained on a Stratagene Mx3000P real-time PCR instrument. The primer sets for all three genes demonstrate the ability to detect a wide range of signals from the serially diluted samples. PPIA = peptidylprolyl isomerase A (cyclophilin A; high-abundance); HPRT = hypoxanthine phosphoribosyltransferase I (medium-abundance); PBGD = porphobilinogen deaminase (low-abundance). Slope and R2 values refer to the line determinedby plotting C_t values versus template quantity

Back

636692: qPCR Human Reference cDNA, Oligo(dT)-primed

cDNAs prepared from a mixture of total RNAs collected from adult normal human tissues. This cDNA mixture has been chosen to represent a broad range of expressed genes.

qPCR Human Reference cDNA serves as a standard for accurate and reproducible comparison of gene expression data using qPCR (quantitative PCR) as well as a positive control template for validation of gene primer design. Enough cDNA is supplied for either 25 or 100 reactions requiring 2 μl of sample per reaction; the number of reactions is based on a 50 μl reaction volume.


The sample was oligo(dT) primed.

Back

High-, medium- and low-abundance gene targets are easily detected in qPCR Human Reference cDNA

High-, medium- and low-abundance gene targets are easily detected in qPCR Human Reference cDNA. Oligo dT-primed (Panels A–C) and random oligo-primed (Panels D–F) qPCR Human Reference cDNA samples were each serially diluted fivefold such that the final quantities of cDNA template in the qPCR reactions were 20 ng, 4 ng, 800 pg, 160 pg, 32 pg,and 6.4 pg. Data were obtained on a Stratagene Mx3000P real-time PCR instrument. The primer sets for all three genes demonstrate the ability to detect a wide range of signals from the serially diluted samples. PPIA = peptidylprolyl isomerase A (cyclophilin A; high-abundance); HPRT = hypoxanthine phosphoribosyltransferase I (medium-abundance); PBGD = porphobilinogen deaminase (low-abundance). Slope and R2 values refer to the line determinedby plotting C_t values versus template quantity

Back

636693: qPCR Human Reference cDNA, Oligo(dT)-primed

cDNAs prepared from a mixture of total RNAs collected from adult normal human tissues. This cDNA mixture has been chosen to represent a broad range of expressed genes.

qPCR Human Reference cDNA serves as a standard for accurate and reproducible comparison of gene expression data using qPCR (quantitative PCR) as well as a positive control template for validation of gene primer design. Enough cDNA is supplied for either 25 or 100 reactions requiring 2 μl of sample per reaction; the number of reactions is based on a 50 μl reaction volume.

The sample was random 9-mer primed.

Back

High-, medium- and low-abundance gene targets are easily detected in qPCR Human Reference cDNA

High-, medium- and low-abundance gene targets are easily detected in qPCR Human Reference cDNA. Oligo dT-primed (Panels A–C) and random oligo-primed (Panels D–F) qPCR Human Reference cDNA samples were each serially diluted fivefold such that the final quantities of cDNA template in the qPCR reactions were 20 ng, 4 ng, 800 pg, 160 pg, 32 pg,and 6.4 pg. Data were obtained on a Stratagene Mx3000P real-time PCR instrument. The primer sets for all three genes demonstrate the ability to detect a wide range of signals from the serially diluted samples. PPIA = peptidylprolyl isomerase A (cyclophilin A; high-abundance); HPRT = hypoxanthine phosphoribosyltransferase I (medium-abundance); PBGD = porphobilinogen deaminase (low-abundance). Slope and R2 values refer to the line determinedby plotting C_t values versus template quantity

cDNAs prepared from a mixture of total RNAs collected from adult normal human tissues. This cDNA mixture has been chosen to represent a broad range of expressed genes.

qPCR Human Reference cDNA serves as a standard for accurate and reproducible comparison of gene expression data using qPCR (quantitative PCR) as well as a positive control template for validation of gene primer design. Enough cDNA is supplied for either 25 or 100 reactions requiring 2 μl of sample per reaction; the number of reactions is based on a 50 μl reaction volume.

The sample was random 9-mer primed.

Back

High-, medium- and low-abundance gene targets are easily detected in qPCR Human Reference cDNA

High-, medium- and low-abundance gene targets are easily detected in qPCR Human Reference cDNA. Oligo dT-primed (Panels A–C) and random oligo-primed (Panels D–F) qPCR Human Reference cDNA samples were each serially diluted fivefold such that the final quantities of cDNA template in the qPCR reactions were 20 ng, 4 ng, 800 pg, 160 pg, 32 pg,and 6.4 pg. Data were obtained on a Stratagene Mx3000P real-time PCR instrument. The primer sets for all three genes demonstrate the ability to detect a wide range of signals from the serially diluted samples. PPIA = peptidylprolyl isomerase A (cyclophilin A; high-abundance); HPRT = hypoxanthine phosphoribosyltransferase I (medium-abundance); PBGD = porphobilinogen deaminase (low-abundance). Slope and R2 values refer to the line determinedby plotting C_t values versus template quantity

Back

639654: qPCR Human Reference cDNA, Random-primed

A mixture of total RNAs from a collection of adult human tissues, chosen to represent a broad range of expressed genes. Both male and female donors are represented.

qPCR Reference Total RNA serves as a standard for the accurate and reproducible comparison of gene expression data using real-time quantitative PCR (qPCR). It may also be used as a source of positive control templates for validating qPCR primer designs.

Total RNA was isolated by a modified guanidinium thiocyanate method.

Back

High-, medium- and low-abundance gene targets are easily detected in qPCR Human Reference cDNA

High-, medium- and low-abundance gene targets are easily detected in qPCR Human Reference cDNA. Oligo dT-primed (Panels A–C) and random oligo-primed (Panels D–F) qPCR Human Reference cDNA samples were each serially diluted fivefold such that the final quantities of cDNA template in the qPCR reactions were 20 ng, 4 ng, 800 pg, 160 pg, 32 pg,and 6.4 pg. Data were obtained on a Stratagene Mx3000P real-time PCR instrument. The primer sets for all three genes demonstrate the ability to detect a wide range of signals from the serially diluted samples. PPIA = peptidylprolyl isomerase A (cyclophilin A; high-abundance); HPRT = hypoxanthine phosphoribosyltransferase I (medium-abundance); PBGD = porphobilinogen deaminase (low-abundance). Slope and R2 values refer to the line determinedby plotting C_t values versus template quantity

Back

636690: qPCR Human Reference Total RNA

Mixture of total RNAs from a collection of adult rat tissues, chosen to represent a broad range
of expressed genes. Both male and female animals are represented. Rat Universal Reference Total RNA serves as a standard for the comparison of gene expression data and is useful as an internal control for microarray expression profiling experiments.

Total RNA was isolated by a modified guanidinium thiocyanate method.

Back

636658: Rat Universal Reference Total RNA

Back

Clontech Rat Universal Reference Total RNA demonstrates more than 90% gene coverage

Clontech Rat Universal Reference Total RNA demonstrates more than 90% gene coverage. Cy3 labeled probes were generated using our Rat Reference Total RNA (Row A), another vendor’s reference total RNA (Row B), a random RNA mixture (Row C), and an individual RNA sample (Row D). Probes were hybridized to a glass rat array containing 3,766 long oligonucleotides. Expression results were analyzed using GeneSpring software (version 5.0) to cluster genes according to their expression patterns. Varying colors reflect the ratio of the intensity of any gene on each array to its median intensity across all arrays. The red and blue colors reflect high and low ratios, respectively. Our results indicate that the Reference Total RNA has more than 90% gene coverage with even distribution and outperforms another vendor’s universal RNA mixture.