We use cookies to improve your browsing experience and provide meaningful content. Read our cookie policy. Accept
  •  Customer Login
  • Register
  •  View Cart (0)
  •  Customer Login
  • Register
  •  View Cart (0)

  • Products
  • Learning centers
  • Services & Support
  • Areas of interest
  • About

Close

  • ‹ Back to Technology and application overviews
  • SMART technology
  • Single-cell mRNA-seq
  • Total RNA-seq
  • Biomarker discovery with RNA-seq
  • SMART-Seq PLUS solutions
  • Whole genome amplification from single cells
  • Cell-free nucleic acid sequencing
  • ThruPLEX HV data sheet
  • Sequencing depth for ThruPLEX Tag-seq
Brain cells Customer interview: unraveling the brain with SMART-Seq v4
SMART-Seq v4 product page SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing
Home › Learning centers › Next-generation sequencing › Technology and application overviews › Single-cell mRNA-seq

Next-generation sequencing

  • Product line overview
  • Technical notes
    • Single-cell RNA- and DNA-seq
      • All-in-one cDNA synthesis and library prep from single cells
      • Highest sensitivity for single-cell mRNA-seq
      • Stranded libraries from single cells
      • Streamlined single-cell mRNA-seq
      • Full-length mRNA libraries from single cells (SMART-Seq v4)
      • 3' mRNA libraries from single cells (SMART-Seq v4 3' DE Kit)
      • Full-length mRNA libraries from single cells for Fluidigm C1 (SMART-Seq v4)
      • Full-length single-cell library method comparison
      • High-resolution CNV detection using PicoPLEX Gold DNA-Seq
      • Next-gen WGA method for CNV and SNV detection from single cells
    • RNA-seq
      • Stranded libraries from picogram-input total RNA (v3)
      • Automation-friendly, all-in-one cDNA synthesis and library prep
      • All-in-one cDNA synthesis and library prep from ultra-low RNA inputs
      • Stranded libraries from picogram-input total RNA (v2)
      • Stranded libraries from FFPE inputs (v2)
      • Stranded libraries from 100 ng - 1 ug total RNA
      • Stranded libraries from 100 pg-100 ng total RNA
      • Stranded libraries from picogram-input total RNA (v1)
      • Stranded RNA-seq competitor kit comparison
      • Nonstranded libraries from FFPE inputs
      • Sensitive capture of full-length transcript information with targeted RNA-seq
    • DNA-seq
      • ThruPLEX HV outperforms NEBNext Ultra II
      • Accurate detection of low-frequency variants using molecular tags
      • Streamlined DNA-seq from challenging samples
      • Low cell number ChIP-seq using SMARTer ThruPLEX
      • Cell-free DNA sequencing
      • Sequencing analysis of low-frequency mutations in cfDNA
      • DNA-seq from FFPE samples
      • Low-input whole-exome sequencing
      • Tag-seq variant detection
      • Low-volume DNA shearing for SMARTer ThruPLEX library prep
    • Immune profiling
      • BCR repertoire profiling from human samples (bulk)
      • Improved TCR repertoire profiling from human samples (bulk)
      • TCR repertoire profiling from human samples (single cells)
      • TCR repertoire profiling from human samples (bulk)
      • TCR repertoire profiling from mouse samples (bulk)
      • BCR repertoire profiling from mouse samples (bulk)
    • Epigenetics and smRNA-seq
      • Full-length small RNA libraries
      • ChIP-seq libraries for transcription factor analysis
      • ChIP-seq libraries from ssDNA
      • Methylated DNA-seq
  • Featured kits
  • Technology and application overviews
    • SMART technology
    • Single-cell mRNA-seq
    • Total RNA-seq
    • Biomarker discovery with RNA-seq
    • SMART-Seq PLUS solutions
    • Whole genome amplification from single cells
    • Cell-free nucleic acid sequencing
    • ThruPLEX HV data sheet
    • Sequencing depth for ThruPLEX Tag-seq
  • FAQs and tips
    • RNA-seq FAQs
    • RNA-seq tips
    • Positive and negative controls in scRNA-seq
    • DNA-seq FAQs
    • ChIP-seq FAQs
    • Indexing FAQs
  • DNA-seq protocols
    • Using UMIs with ThruPLEX Tag-Seq HV
    • Exome capture with Illumina Nextera Rapid Capture
    • Targeted capture with Roche NimbleGen SeqCap EZ
    • Targeted capture with IDT xGen panels
    • Targeted capture with Agilent SureSelectXT
    • Targeted capture with Agilent SureSelectXT2
    • Targeted capture with Agilent SureSelectQXT
    • Plasma preparation for ThruPLEX Plasma-Seq workflow
  • Bioinformatics resources
    • Cogent NGS Immune Profiler Software
      • Cogent NGS Immune Profiler Software notices
      • Cogent NGS Immune Profiler Software videos
    • ICELL8 scTCR Analyzer
    • SMARTer Human scTCR Demultiplexer
    • Cogent NGS Analysis Pipeline
      • Cogent NGS Analysis Pipeline v1.0 User Manual
      • Cogent NGS Analysis Pipeline notices
    • Cogent NGS Discovery Software
      • Cogent NGS Discovery Software v1.0 User Manual
      • Cogent NGS Discovery Software notices
    • SMART-Seq DE3 Demultiplexer
  • Newsletters
    • 2020 NGS newsletter
  • Webinars
    • Single-Cell Workshop
    • TCR-seq methods: when to use which
    • Taking single-cell RNA-seq by STORM
    • Immunogenomics to accelerate immunotherapy
    • Total RNA sequencing of liquid biopsies
    • MeD-Seq, a novel method to detect DNA methylation
    • Automating full-length single-cell RNA-seq libraries
    • Single-cell DNA-seq
    • Single-cell whole transcriptome analysis
  • Citations
    • Epigenetics citations
    • Microbiome citations
    • PicoPLEX citations
    • SMARTer RNA-seq citations
    • ThruPLEX DNA-seq citations
    • ThruPLEX Plasma-seq citations
  • Posters
New products
Need help?
Contact Sales
Brain cells Customer interview: unraveling the brain with SMART-Seq v4
SMART-Seq v4 product page SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing

Single-cell mRNA-seq overview

The industry standard for ultra-low input RNA-seq

Innovative research continuously pushes the boundaries of new technologies in a quest for compelling results, but it still requires the reagents, tools, and technical support to ensure success. In the world of single-cell and ultra-low input RNA-seq, Takara Bio provides you with the only SMART choice. With these tools, researchers can surpass the current limits of RNA-seq sensitivity.

This overview describes:

  • SMARTer Ultra low solutions for single-cell mRNA-seq
  • Differences between the generations of SMARTer Ultra Low kits

Why study single cells?

One of the most powerful ways to learn about cellular responses to stimuli is through studying genome-wide changes in transcription; however, a population of cells can conceal biologically relevant differences in the transcriptomes of individual cells. Many publications have shown that a single-cell or ultra-low input mRNA-seq approach can give new insights into diverse research areas such as tumor heterogeneity, cell lineage characterization, development, and the diversity of cells in a population.

What are the challenges of single-cell mRNA-seq?

Studying rare or precious samples—including stem cells, circulating tumor cells, and tissue biopsies—requires extraordinary sensitivity and reproducibility. Producing unbiased cDNA libraries for mRNA-seq is a particular challenge due to the limited number of RNA molecules available for reverse transcription in a single cell. A single cell may only contain 10 to 30 pg of RNA, depending on its size and metabolic state.

SMARTer Ultra low solutions for single-cell mRNA-seq

SMARTer Ultra low kits are best-in-class tools for sequencing library preparation from single cells, a few cells, and ultra-low inputs of RNA. These kits rely on oligo(dT) priming and proprietary SMART (Switching Mechanism at 5' end of RNA Template) technology to ensure full-length, unbiased mRNA coverage. Intact cells can be used directly as the input for these kits, guaranteeing high-quality input RNA and full-length cDNA coverage.

  • Ultra-low input: 1–1,000 cells, or 10 pg–10 ng high-quality (RIN >8) total RNA
  • Versatile: cDNA is compatible with Illumina or Ion Torrent platforms
  • Superior performance: A high number of genes identified, full-length transcript data, and minimal rRNA reads
  • Well established: Click to see selected SMARTer citations »

profiles of cDNA libraries generated from single-cell or ultra-low inputs

cDNA libraries generated from single cells or a few cells with SMARTer Ultra low kits show remarkable reproducibility. Intact individual cells or 1,000 cells (HeLa or Jurkat; Panel A and Panel B, respectively) were used as input for the SMARTer Ultra Low Input RNA Kit for Sequencing - v3. The cDNA samples were analyzed on an Agilent 2100 Bioanalyzer. The single main peak indicates the purity and yield of the cDNA (the additional peak at ~1 kb in the HeLa cell samples is the contribution of a single highly expressed gene, FTH1).
 

correlation between qPCR and NGS data

Expression data produced using SMARTer Ultra low kits highly correlates with quantitative PCR (qPCR) data reported by the MicroArray Quality Control (MAQC) project. Scatter plots were used to compare differential expression data obtained using the SMARTer Ultra Low RNA Kit for Illumina Sequencing (from 1 ng total RNA) and qPCR data available for Universal Human Reference Total RNA (UHR) and Human Brain Reference RNA. The differential expression of ~700 genes showed a correlation value of 0.94, demonstrating that the sequencing results are consistent with previously reported expression data.

Go to the SMARTer Ultra low kits product page »

Differences between the generations of SMARTer Ultra low kits

We are driven to continuously improve our tools for superior single-cell mRNA-seq applications. With the latest, fourth-generation SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing, we have built upon our experience with single-cell mRNA-seq and incorporated SMART-Seq2 innovations, including locked nucleic acid (LNA) technology, along with additional improvements in SMART technology. This kit delivers higher sensitivity, meaning more genes are identified; better reproducibility, especially for transcripts with FPKM <100; and lower background than either the SMART-Seq2 method or any previous SMARTer Ultra low kit. The combination of SMARTer technology and SMART-seq2 innovations is often loosely referred to as SMARTer-Seq chemistry.

Legacy SMARTer Ultra low kits are still available for full-length, unbiased transcriptome coverage.

  • The SMARTer Ultra Low Input RNA Kit for Sequencing - v3 generated libraries with improved sequencing metrics, particularly for GC-rich sequences. This third-generation kit included many modifications from previous generations, including optimized primer and template-switching oligonucleotide concentrations, a different polymerase for library amplification (SeqAmp DNA Polymerase), and allowed for greater sequencing platform versatility.
  • The SMARTer Ultra Low RNA Kit for Illumina Sequencing and SMARTer Ultra Low Input RNA for Illumina Sequencing - HV kit are the first- and second-generation kits, respectively. Both of these kits are based on the SMART-Seq method and use the Advantage 2 DNA polymerase. SMARTer Ultra Low Input RNA for Illumina Sequencing - HV kits differ from the first-generation kit by allowing for a larger input volume (9 µl rather than 1 µl).

Finally, the SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System, IFCs provides an excellent solution for high-throughput mRNA-seq on the Fluidigm C1 platform. This kit is based on the fourth-generation SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing.


Evaluating the SMART-Seq v4 method for mRNA-seq with the Fluidigm C1 | April 2016

In this webinar about the SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System, IFCs, Dr. Charles Gawad (St. Jude Children's Research Hospital) compares high-throughput single-cell mRNA-seq data generated with the Fluidigm C1 system using either the SMART-Seq v4 method or the original v1 kit.

SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System product page

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2020 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Support
  • Contact us
  • Technical support
  • Customer service
  • Shipping & delivery
  • Sales
  • Feedback
Products
  • New products
  • Special offers
  • Instrument & reagent services
  • Corporate development
Learning centers
  • NGS
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
About
  • Our brands
  • Careers
  • Events
  • Blog
  • Need help?
  • Announcements
  • Quality and compliance
  • That's Good Science!
Facebook Twitter  LinkedIn

©2021 Takara Bio Inc. All Rights Reserved.

Region - North America Privacy Policy Terms and Conditions Terms of Use

Top



  • COVID-19 research
  • Drug discovery
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Viral RNA isolation
  • Immune profiling
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Publications
  • Automation systems
  • SmartChip Real-Time PCR System, chips, and reagents
  • Apollo system
  • ICELL8 system and software
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Whole genome amplification
  • Immune profiling
  • Bioinformatics tools
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • RNA extraction and analysis for real-time qPCR
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Human iPS cell gene editing systems
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • cDNA synthesis
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion Cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Cell biology assays
  • Extracellular vesicle isolation
  • Exosome isolation (cell culture)
  • Reporter systems
  • Apoptosis detection kits
  • Epigenetics
  • Signal transduction
  • Gene function
  • Gene editing
  • Fluorescent proteins
  • Viral transduction
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • Transfection reagents
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISAs
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • New products
  • Special offers
  • Extracellular vesicle purification kit samples
  • GoStix Plus special offers
  • PCR samples
Vaccine development

Vaccine development

The rapid spread of severe infections by viruses such as SARS-CoV-2, HIV, H1N1, Ebola, and Zika has highlighted the critical need for the rapid development of vaccines against previously unknown pathogens to deal with pandemics such as COVID-19 effectively.

Takara Bio is proud to be on the front line in the fight to defeat the novel coronavirus by enabling innovative vaccine development. This section discusses tools and techniques to overcome the challenges faced during the vaccine development process.

Learn how our products help speed up vaccine development

  • Automation systems
  • SmartChip Real-Time PCR System introduction
  • Apollo library prep system introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • Product line overview
  • Technical notes
  • FAQs and tips
  • Bioinformatics resources
  • Newsletters
  • Webinars
  • Citations
  • Posters
  • Real-time PCR
  • Product finder
  • Reaction size guidelines for qPCR
  • Real-time PCR products brochure
  • Real-time PCR tutorial videos
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Overview
  • Technical notes
  • FAQs
  • Stem cell research
  • Protocols
  • Applications
  • Technical notes
  • Webinars
  • Videos
  • Citations
  • Nucleic acid purification
  • Product finder
  • Plasmid purification
  • Genomic DNA purification
  • DNA/RNA cleanup and extraction
  • Automated DNA and RNA purification
  • RNA purification
  • Hard-to-lyse samples
  • cDNA synthesis
  • PCR
  • Citations
  • Selection guides
  • PCR enzyme brochure
  • Technical notes
  • PCR FAQs
  • Cloning
  • In-Fusion Cloning: general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and tech notes
  • Cell biology assays
  • Extracellular vesicle isolation
  • Technical notes
  • FAQs
  • Citations
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Transfection reagents
  • Fluorescent proteins
  • Protein research
  • Capturem technology
  • Antibody purification
  • His-tag purification
  • Phosphoprotein and glycoprotein purification
  • Mass spectrometry digestion reagents
  • Matchmaker Gold yeast two-hybrid systems
  • Antibodies and ELISA
Capturem Trypsin for a rapid, efficient mass spectometry workflow at room temperature.

Speed up your mass spec workflow

Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis. This product utilizes our novel Capturem technology in a spin column format with membrane-immobilized trypsin. Capturem Trypsin Columns may be used to completely digest protein samples in less than a minute with digestion efficiencies (protein coverage) comparable to or better than those obtained using in-solution trypsin digestion.

Capturem trypsin technology

  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip services
  • OEM & custom enzyme manufacturing
  • Services
  • Quality
  • Expertise
  • OEM enzyme FAQs
  • Custom enzyme samples
  • Exploring OEM and custom enzyme partnerships
  • Stem cell services
  • Clinical-grade stem cell services
  • Research-grade stem cell services
  • Outsourcing stem cell-based disease model development
  • Gene and cell therapy manufacturing services
  • Services
  • Facilities
  • Our process
  • Resources
  • Customer service
  • Sales
  • Make an appointment with your sales rep
  • Shipping & delivery
  • Technical support
  • Feedback
  • Online tools
  • GoStix Plus FAQs
  • Vector information
  • Vector document overview
  • Vector document finder
  • Corporate development
  • Partnering & OEM solutions
  • In licensing
  • Out licensing
  • Submit a licensing request
  • Webinars
  • NGS: biomarkers and oncology
  • NGS: immunology
  • Stem cells
  • Real-time PCR
  • Gene function
  • Protein science
Takara Bio's award-winning GMP-compliant manufacturing facility in Kusatsu, Shiga, Japan.

Partner with Takara Bio!

Takara Bio is proud to offer GMP-grade manufacturing capabilities at our award-winning facility in Kusatsu, Shiga, Japan.

Learn more

  • Vaccine development
  • Characterizing the viral genome and host response
  • Identifying and cloning vaccine targets
  • Expressing and purifying vaccine targets
  • Immunizing mice and optimizing vaccine targets
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Preimplantation genetic testing
Create a web account with us

Log in to enjoy additional benefits

Want to save this information?

An account with takarabio.com entitles you to extra features such as:

•  Creating and saving shopping carts
•  Keeping a list of your products of interest
•  Saving all of your favorite pages on the site*
•  Accessing restricted content

*Save favorites by clicking the star () in the top right corner of each page while you're logged in.

Create an account to get started

  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • Season one
  • Season two
  • Season three
  • Our brands
  • Takara
  • Clontech
  • Cellartis
  • Our history
  • Announcements
  • Events
  • Calendar
  • Conferences
  • Speak with us
  • Careers
  • Trademarks
  • License statements
  • Quality statement
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
  • Need help?
  • Website FAQs
Best-in-class products, expert support, superior value

That's GOOD Science!

What does it take to generate good science? Careful planning, dedicated researchers, and the right tools. At Takara Bio, we thoughtfully develop best-in-class products to tackle your most challenging research problems, and have an expert team of technical support professionals to help you along the way, all at superior value.

Explore what makes good science possible

Evaluating the SMART-Seq v4 method for mRNA-seq with the Fluidigm C1 | April 2016

In this webinar about the SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System, IFCs, Dr. Charles Gawad (St. Jude Children's Research Hospital) compares high-throughput single-cell mRNA-seq data generated with the Fluidigm C1 system using either the SMART-Seq v4 method or the original v1 kit.

SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System product page

 Customer Login
 View Cart (0)
  • Home
  • Products
  • Learning centers
  • Services & Support
  • Areas of interest
  • About
  •  Customer Login
  • Register
  •  View Cart (0)

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Clontech, TaKaRa, cellartis

  • Products
  • COVID-19 research
  • Automation systems
  • Next-generation sequencing
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
  • Antibodies and ELISA
  • Cell biology assays
  • Real-time PCR
  • cDNA synthesis
  • COVID-19 research
  • Drug discovery
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Viral RNA isolation
  • Immune profiling
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Publications
  • Automation systems
  • SmartChip Real-Time PCR System, chips, and reagents
  • Apollo system
  • ICELL8 system and software
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Whole genome amplification
  • Immune profiling
  • Epigenetics and small RNA sequencing
  • NGS accessories
  • Bioinformatics tools
  • Gene function
  • Gene editing
  • Fluorescent proteins
  • Viral transduction
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • ProteoTuner protein control systems
  • iDimerize inducible protein interaction systems
  • Transfection reagents
  • Mammalian expression plasmids
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Human iPS cell gene editing systems
  • Accessories
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Expression vectors & systems
  • Glycobiology
  • Antibodies and immunoprecipitation
  • SDS-PAGE & western blotting
  • Protein sequencing
  • Accessory enzymes
  • PCR
  • Most popular polymerases
  • Standard PCR
  • High-yield PCR
  • High-fidelity PCR
  • Fast PCR
  • Long-range PCR
  • GC rich PCR
  • Direct PCR
  • PCR master mixes
  • Custom business friendly and automation-ready solutions
  • Molecular diagnostic products
  • GMP-grade products
  • Application-specific PCR
  • Other PCR-related products
  • PCR thermal cyclers
  • Cloning
  • In-Fusion Cloning
  • Competent cells
  • Ligation kits
  • Mutagenesis kits
  • Ligation enzymes
  • Restriction enzymes
  • Modifying enzymes
  • X-Gal and IPTG
  • Linkers, primers, and cloning vectors
  • Agarose gel electrophoresis
  • Nucleic acid extraction
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • RNA cleanup kits
  • Viral DNA and RNA purification kits
  • Accessories and components
  • Antibodies and ELISA
  • Primary antibodies and ELISAs by research area
  • Secondary antibodies
  • Antibody and ELISA accessories
  • Fluorescent protein antibodies
  • Cell biology assays
  • Extracellular vesicle isolation
  • Exosome isolation (cell culture)
  • Reporter systems
  • Apoptosis detection kits
  • Epigenetics
  • Cell biology reagents
  • RNA interference
  • Cell-culture accessories
  • Signal transduction
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • Real-time PCR primer sets
  • References and standards for qPCR
  • RNA extraction and analysis for real-time qPCR
  • Application-specific qPCR
  • cDNA synthesis
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • cDNA synthesis accessories
  • Learning centers
  • Automation systems
  • Next-generation sequencing
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
  • Antibodies and ELISA
  • Cell biology assays
  • Real-time PCR
  • cDNA synthesis
  • Automation systems
  • SmartChip Real-Time PCR System introduction
  • Apollo library prep system introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • Product line overview
  • Technical notes
  • Featured kits
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Bioinformatics resources
  • Newsletters
  • Webinars
  • Citations
  • Posters
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Transfection reagents
  • Fluorescent proteins
  • Stem cell research
  • Protocols
  • Applications
  • Technical notes
  • Posters
  • Webinars
  • Videos
  • FAQs
  • Citations
  • Selection guides
  • Overview
  • Protein research
  • Capturem technology
  • Antibody purification
  • His-tag purification
  • Other tag purification
  • Phosphoprotein and glycoprotein purification
  • Mass spectrometry digestion reagents
  • Matchmaker Gold yeast two-hybrid systems
  • Expression systems
  • PCR
  • Citations
  • Selection guides
  • PCR enzyme brochure
  • Technical notes
  • PCR FAQs
  • Go green with lyophilized enzymes
  • LA PCR technology
  • Cloning
  • In-Fusion Cloning: general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and tech notes
  • Sign up to stay updated
  • Traditional molecular cloning
  • Nucleic acid purification
  • Product finder
  • Plasmid purification
  • Genomic DNA purification
  • DNA/RNA cleanup and extraction
  • Parallel DNA, RNA & protein
  • Automated DNA and RNA purification
  • RNA purification
  • Hard-to-lyse samples
  • Antibodies and ELISA
  • Osteocalcin focus
  • Cell biology assays
  • Extracellular vesicle isolation
  • Technical notes
  • FAQs
  • Citations
  • Real-time PCR
  • Product finder
  • Reaction size guidelines for qPCR
  • Real-time PCR products brochure
  • Real-time PCR tutorial videos
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Overview
  • Technical notes
  • FAQs
  • cDNA synthesis
  • Premium total and poly A+ RNA
  • SMARTer RACE 5'/3' Kit—advances in SMARTer PCR cDNA synthesis
  • Cloning antibody variable regions
  • Services & Support
  • Instrument services
  • OEM & custom enzyme manufacturing
  • Stem cell services
  • Gene and cell therapy manufacturing services
  • Customer service
  • Technical support
  • Sales
  • Shipping & delivery
  • Feedback
  • Corporate development
  • Webinars from Takara Bio
  • Vector information
  • Online tools
  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip services
  • OEM & custom enzyme manufacturing
  • Services
  • Quality
  • Expertise
  • OEM enzyme FAQs
  • Custom enzyme samples
  • Exploring OEM and custom enzyme partnerships
  • Stem cell services
  • Clinical-grade stem cell services
  • Research-grade stem cell services
  • Outsourcing stem cell-based disease model development
  • Gene and cell therapy manufacturing services
  • Services
  • Facilities
  • Our process
  • Resources
  • Sales
  • Make an appointment with your sales rep
  • Corporate development
  • Partnering & OEM solutions
  • In licensing
  • Out licensing
  • Submit a licensing request
  • Webinars from Takara Bio
  • NGS: biomarkers and oncology
  • NGS: immunology
  • Stem cells
  • Real-time PCR
  • Gene function
  • Protein science
  • Vector information
  • Vector document overview
  • Vector document finder
  • Online tools
  • GoStix Plus FAQs
  • Areas of interest
  • Pathogen detection
  • Vaccine development
  • Cancer research
  • Immunotherapy research
  • Alzheimer's disease research
  • Reproductive health technologies
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Vaccine development
  • Characterizing the viral genome and host response
  • Identifying and cloning vaccine targets
  • Expressing and purifying vaccine targets
  • Immunizing mice and optimizing vaccine targets
  • Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Preimplantation genetic testing
  • About
  • BioView blog
  • That's Good Science!
  • Our brands
  • Our history
  • Announcements
  • Events
  • Careers
  • Trademarks
  • License statements
  • Quality and compliance
  • Takara Bio affiliates & distributors
  • Need help?
  • Website FAQs
  • DSS Takara Bio India Pvt. Ltd : Manufacturing
  • Our partners
  • Special offers
  • New products
  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • Season one
  • Season two
  • Season three
  • Our brands
  • Takara
  • Clontech
  • Cellartis
  • Events
  • Calendar
  • Conferences
  • Speak with us
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
  • Special offers
  • Extracellular vesicle purification kit samples
  • GoStix Plus special offers
  • PCR samples
  • Products
  • Learning centers
  • Services & Support
  • Areas of interest
  • About