His-tagged protein purification from crude cell lysates—TALON CellThru

TALON CellThru is a novel IMAC resin for purifying his-tagged proteins from crude cell lysates, sonicates, and fermentation harvests. This resin uses the same proprietary ligand as our TALON Metal Affinity Resin but has larger beads (300–500 µm) which permit cellular debris to flow through without centrifugation. TALON CellThru Resin captures his-tagged protein directly from crude lysates in one quick step, minimizing protein degradation and generating higher yields of purified protein than conventional strategies.

TALON CellThru Resin is ideal for purifying membrane-bound proteins or multi-protein complexes, which typically pellet with the cell debris during the lysate clarification step and are not available for binding to the resin. Because crude, nonclarified lysates can be applied directly to the resin, you can skip the centrifugation step, allowing membrane-bound proteins and multi-protein complexes to remain in the lysate where they can bind to the resin.

The resin consists of large agarose beads that can be packed into standard chromatography columns with large-pore frits to prevent column blockage. Particulate material flows between the beads while the soluble protein product is captured by the ion-exchange functional groups attached to the beads. The resin is ideal for use in expanded bed chromatography for recovering even higher amounts of protein and is recommended for applications such as high-throughput purification of his-tagged proteins from crude extracts. We also offer CellThru 10-ml Disposable Columns (with a filter pore size of 90–130 µm) that allow cellular debris to flow through easily.