Myc-tag and HA-tag vectors
These mammalian expression vectors allow you to express a protein of interest fused to either the c-Myc or hemagglutinin (HA) epitope tag. c-Myc and HA are well-characterized and highly immunoreactive tags, and thus are ideal for co-immunoprecipitation studies but are also easily detected via Western blot. Moreover, c-Myc and HA are small tags and thus unlikely to interfere with the function of your protein.
These mammalian expression vectors allow you to express a protein of interest fused to either the c-Myc or hemagglutinin (HA) epitope tag. c-Myc and HA are well-characterized and highly immunoreactive tags, and thus are ideal for co-immunoprecipitation studies but are also easily detected via Western blot. Moreover, c-Myc and HA are small tags and thus unlikely to interfere with the function of your protein.
The pCMV vectors use the CMV promoter for constitutive, high-level expression in a wide variety of mammalian cells. The myc-tagged protein of interest can be detected by either Western blot analysis or fluorescence microscopy using an anti-myc antibody.
The pCMV-Myc & pCMV-HA Vector Set (Cat. # 631604) includes c-Myc monoclonal and HA-Tag polyclonal antibodies, which can also be used in Western blot, ELISA, or immunostaining assays. A sequencing primer is also included in the set to confirm that the insert is in the correct reading frame.
Overview
- Co-immunoprecipitation of myc-tagged and HA-tagged proteins in mammalian cells
- Strong constitutive expression
- Confirm yeast two-hybrid interactions in mammalian cells
- Strong clean bands via Western blot
More Information
Applications
- Co-immunoprecipitation studies
- Confirmation of protein-protein interactions
- Western blotting
- Epitope-tagged expression
Sequence information
HA tag sequence: ACC ATG TAC CCA TAC GAT GTT CCA GAT TAC GCT
Myc tag sequence: ATG GCA TCA ATG CAG AAG CTG ATC TCA GAG GAG GAC CTG
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Ultra-high expression of recombinant proteins
High expression of functional OKT3 monoclonal antibodies in mammalian cells
pHEK293 Ultra Expression Vectors provide ultra-high expression of recombinant proteins, including antibodies, in mammalian cells.
Tech note
A revolutionary solution for recombinant protein purification
Learn about a novel solution for recombinant protein purification from mammalian and bacterial cell lysates.
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