Deoxynucleotide triphosphates
Our dNTPs for PCR are ≥98% pure and are tested for quality control in a variety of applications. Individual dNTPs are supplied at a concentration of 100 mM and can be diluted with water or buffer as needed. The dNTP Set (Cat. # 4025) provides one of each of the four deoxynucleotide triphosphates.
Premixed dNTPs are also available (Cat. # 4030). The mixture contains each dNTP at a concentration of 2.5 mM. It offers convenience, minimizes pipetting, and can be added directly to amplification reactions.
More Information
Applications
- DNA amplification (PCR)
- RT-PCR
- cDNA synthesis
- DNA cloning, sequencing, and labeling
Storage
–20°C
Avoid repeated freeze-thaw cycles. Once thawed, aliquot into separate tubes and store at –20°C.
Buffer
Sodium salt aqueous solution (pH 7.0–9.0)
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Find answers to your PCR questions
Primer design
Frequently asked questions about primer design for successful PCR.
Optimization
Frequently asked questions about PCR optimization.
Troubleshooting
Frequently asked questions about troubleshooting your PCR problems.
Applications and conditions
Frequently asked questions about general and specific applications for PCR and which polymerases to choose.
Shipping, storage, and handling
Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.
Avoid DNA contamination in PCR
There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.
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