LA Taq PCR kit
The LA PCR Kit, Version 2.1 contains all the reagents needed for amplification of long DNA templates, enabling routine amplification of products up to 20 kb in length, including GC-rich amplicons. For some DNA templates, amplification of up to 48 kb is possible. This long-range PCR kit contains TaKaRa LA Taq DNA Polymerase, buffers, MgCl2, dNTPs, molecular weight markers, and control templates, plus corresponding primers to ensure optimal PCR performance during long PCR.
Overview
- Genomic DNA template amplification up to 30 kb, with included control reactions
- Lambda DNA template amplification up to 48 kb, with included control reactions
- Longer and more accurate PCR products
- Specialized 2X GC buffer provided for templates with high GC content or extensive secondary structure
More Information
PCR products
PCR products generated with LA Taq DNA Polymerase contain a mixture of 3'-A overhangs and blunt ends, resulting in >80% cloning efficiency in T-vectors. However, the efficiency of cloning longer products (>5 kb) into T-vectors is quite low.
Bulk, custom, and OEM information
If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Find answers to your PCR questions
Primer design
Frequently asked questions about primer design for successful PCR.
Optimization
Frequently asked questions about PCR optimization.
Troubleshooting
Frequently asked questions about troubleshooting your PCR problems.
Applications and conditions
Frequently asked questions about general and specific applications for PCR and which polymerases to choose.
Shipping, storage, and handling
Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.
Avoid DNA contamination in PCR
There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.
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