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Ex Taq DNA polymerase—a robust PCR enzyme with proofreading activity

High yield PCR

TaKaRa Ex Taq DNA Polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3'-to-5' exonuclease, for high-sensitivity, high-efficiency PCR reactions. It can also be used for long-range PCR (up to 20 kb from genomic DNA templates and up to 30 kb from lambda DNA templates). Ex Taq polymerase has a higher fidelity than standard Taq with a mutation rate approximately 4.5 times lower, as determined by the Kunkel method. Ex Taq polymerase is supplied with optimized 10X buffer (with or without Mg2+) and dNTPs.

TaKaRa Ex Taq DNA Polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3'-to-5' exonuclease, for high-sensitivity, high-efficiency PCR reactions. It can also be used for long-range PCR (up to 20 kb from genomic DNA templates and up to 30 kb from lambda DNA templates). Ex Taq polymerase has a higher fidelity than standard Taq with a mutation rate approximately 4.5 times lower, as determined by the Kunkel method. Ex Taq polymerase is supplied with optimized 10X buffer (with or without Mg2+) and dNTPs.

In routine PCR applications, using Ex Taq polymerase and the Ex Taq buffer system results in higher yields of PCR products as compared to standard Taq DNA polymerase.

Several other formats of Ex Taq are available:

  • An antibody-mediated hot-start version—for preventing non-specific amplification from mispriming during reaction setup.
  • A 2X PCR master mix contains Ex Taq enzyme, reaction buffer (with Mg2+), and dNTPs—for a simple PCR setup and minimal pipetting steps. A loading-dye added versions, PerfectShot Ex Taq DNA Polymerase, is also available for convenient loading of PCR products in agarose gels directly.
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Cat. # Product Size License Quantity Details
RR001A TaKaRa Ex Taq® DNA Polymerase 250 Units *

TaKaRa Ex Taq DNA Polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3'-to-5' exonuclease, for high-sensitivity, high-efficiency PCR. Ex Taq can also be used for long-range PCR (up to 20 kb from genomic DNA templates and up to 30 kb from lambda DNA templates). Separate tubes of Mg2+ plus buffer and dNTP mix are supplied with the polymerase.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data Resources
RR001B TaKaRa Ex Taq® DNA Polymerase 1,000 Units *

TaKaRa Ex Taq DNA Polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3'-to-5' exonuclease, for high-sensitivity, high-efficiency PCR. Ex Taq can also be used for long-range PCR (up to 20 kb from genomic DNA templates and up to 30 kb from lambda DNA templates). Separate tubes of Mg2+ plus buffer and dNTP mix are supplied with the polymerase. Cat. # RR001B contains 4 of Cat. # RR001A. Please refer to Cat. # RR001A for complete product documentation and resources.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data
RR001C TaKaRa Ex Taq® DNA Polymerase 3,000 Units *

TaKaRa Ex Taq DNA Polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3'-to-5' exonuclease, for high-sensitivity, high-efficiency PCR. Ex Taq can also be used for long-range PCR (up to 20 kb from genomic DNA templates and up to 30 kb from lambda DNA templates). Separate tubes of Mg2+ plus buffer and dNTP mix are supplied with the polymerase. Cat. # RR001C contains 12 of Cat. # RR001A. Please refer to Cat. # RR001A for complete product documentation and resources.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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RR01AM TaKaRa Ex Taq® DNA Polymerase (Mg2+ free buffer) 250 Units *

TaKaRa Ex Taq DNA Polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3'-to-5' exonuclease, for high-sensitivity, high-efficiency PCR. Ex Taq can also be used for long-range PCR (up to 20 kb from genomic DNA templates and up to 30 kb from lambda DNA templates). Separate tubes of Mg2+ free buffer, MgCl2, and dNTPs are supplied with the polymerase.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data Resources
RR01BM TaKaRa Ex Taq® DNA Polymerase (Mg2+ free buffer) 1,000 Units *

TaKaRa Ex Taq DNA Polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3'-to-5' exonuclease, for high-sensitivity, high-efficiency PCR. Ex Taq can also be used for long-range PCR (up to 20 kb from genomic DNA templates and up to 30 kb from lambda DNA templates). Separate tubes of Mg2+ free buffer, MgCl2, and dNTPs are supplied with the polymerase. Cat. # RR01BM contains 4 of Cat. # RR01AM. Please refer to Cat. # RR01AM for complete product documentation and resources.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data
RR01CM TaKaRa Ex Taq® DNA Polymerase (Mg2+ free buffer) 3,000 Units *

TaKaRa Ex Taq DNA Polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3'-to-5' exonuclease, for high-sensitivity, high-efficiency PCR. Ex Taq can also be used for long-range PCR (up to 20 kb from genomic DNA templates and up to 30 kb from lambda DNA templates). Separate tubes of Mg2+ free buffer, MgCl2, and dNTPs are supplied with the polymerase. Cat. # RR01CM contains 12 of Cat. # RR01AM. Please refer to Cat. # RR01AM for complete product documentation and resources.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

Primer design FAQs Primer design FAQs
Optimization FAQs Optimization FAQs
Troubleshooting FAQs Troubleshooting FAQs

Overview

  • DNA-proofreading activity allows for high-fidelity and high-sensitivity reactions
  • Obtain a wide range of PCR products (<100 bp to 20 kb for genomic DNA targets)
  • Optimized 10X Buffer and dNTPs are included—less optimization and more reproducible results than Taq

More Information

Applications

  • Ideal for high-fidelity, high-yield endpoint PCR

PCR products

Most PCR products (approximately 80%) amplified with Takara Ex Taq have a 3'-terminal adenosine (A), and therefore PCR products can be used directly for cloning into T-vectors (TA cloning). It is possible to clone the product into blunt-end vectors after blunting and phosphorylation.

Bulk, custom, and OEM information

If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

1.17 | Heading into bat caves on a rescue mission

Bats may have a "spooky" reputation, but one bat disease is causing widespread fear among wildlife biologists: White Nose Syndrome caused by the fungus Geomyces destructans. With a mortality rate near 100%, the disease is devastating bat populations. Daniel Lindner and colleagues used Takara Ex Taq polymerase to screen for G. destructans in soil samples from bat hibernacula, gathering data and developing testing methods to help bats everywhere. That's Good Science!

Learn about featured products

See what our customers are saying about Takara Ex Taq DNA Polymerase!

"Takara Ex Taq amplify better than pfu from Stratagene, and also has high fidelity. I prefer Ex Taq than pfu."
—Qin Yan, DUKE UNIVERSITY
"I used it for TOPO cloning, worked well!"
—Daad Abi-Ghanem,  IMMUNOLOGY CONSULTANTS LABORATORY INC
"We were very pleased to find that it was the only enzyme that provided robust amplification of single genes housed on single copy number plasmids. We didn't need to use very much template at all to do this reaction which was even better since our templte was from a whole genomic DNA preparation that does not enrich for the plasmids."
—Viveka Vadyvaloo, WASHINGTON STATE UNIVERSITY
"In order to make an overexpressing plasmid, I used PrimeScript 1st Strand cDNA Synthesis kit and Takara Ex Taq DNA Polymerase Hot Start Version. Cloning was perfect. They get easier to make plasmids."
—Koji Ueno, UNIV OF CALIF SAN FRANCISCO

Find answers to your PCR questions

Primer design

Frequently asked questions about primer design for successful PCR.

Optimization

Frequently asked questions about PCR optimization.

Troubleshooting

Frequently asked questions about troubleshooting your PCR problems.

Applications and conditions

Frequently asked questions about general and specific applications for PCR and which polymerases to choose.

Shipping, storage, and handling

Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.

Avoid DNA contamination in PCR

There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.

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