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  • ‹ Back to Ultra-low input RNA-seq
  • SMART-Seq mRNA LP (with UMIs)
  • SMART-Seq mRNA LP and SMART-Seq mRNA
  • SMART-Seq v4 for mRNA-seq
  • SMART-Seq v4 3’ DE for mRNA-seq end counting
  • SMART-Seq DE3 Demultiplexer
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Home › Products › Next-generation sequencing › RNA-seq › Ultra-low input RNA-seq › SMART-Seq mRNA LP and SMART-Seq mRNA

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SSv4 PLUS tech note View data generated with the SSv4 PLUS kit
Technical notes View data for SSv4
Brain cells Customer interview: unraveling the brain with SMART-Seq v4
DNA-RNA-seq (purple) Learn more about our other SMART-Seq PLUS kits
NGS analysis software

SMART-Seq mRNA LP and SMART-Seq mRNA

NOTE: SMART-Seq mRNA LP and SMART-Seq mRNA are equivalent replacements for the SMART-Seq v4 PLUS and SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing with minor updates (see a complete list of kits with new names and the existing kits they will replace here).

The updates (listed below) do not in any way impact the protocols or functional performance of these kits.

  1. SeqAmp CB PCR buffer is provided instead of SeqAmp PCR buffer for improved bead pelleting and resuspension during DNA cleanup
  2. AfaI, 10X AfaI Buffer, and 0.1% BSA are not provided (as they are not required in the protocol)
  3. Input range is extended from 10 pg–10 ng to 10 pg–100 ng total RNA
  4. For added flexibility indexes are not included in the kit, but a choice of indexing primers are sold separately (see Unique Dual Index kits)

NOTE: SMART-Seq mRNA LP and SMART-Seq mRNA are equivalent replacements for the SMART-Seq v4 PLUS and SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing with minor updates (see a complete list of kits with new names and the existing kits they will replace here).

The updates (listed below) do not in any way impact the protocols or functional performance of these kits.

  1. SeqAmp CB PCR buffer is provided instead of SeqAmp PCR buffer for improved bead pelleting and resuspension during DNA cleanup
  2. AfaI, 10X AfaI Buffer, and 0.1% BSA are not provided (as they are not required in the protocol)
  3. Input range is extended from 10 pg–10 ng to 10 pg–100 ng total RNA
  4. For added flexibility indexes are not included in the kit, but a choice of indexing primers are sold separately (see Unique Dual Index kits)

SMART-Seq mRNA and SMART-Seq mRNA LP are two of our most sensitive kits for ultra-low inputs. These kits use oligo(dT) priming to generate high-quality, full-length cDNA directly from multiple intact cells (up to 1,000 cells) or 10 pg–100 ng of total RNA. 

The SMART (Switching Mechanism at 5' End of RNA Template) technology powering cDNA synthesis provides full-length transcript information, enabling analysis of transcript isoforms, gene fusions, point mutations, etc. Additionally, the template-switching oligo (TSO) incorporates the locked nucleic acid (LNA) technology for more efficient template switching. This enables higher sensitivity for the identification of higher numbers of genes relative to other methods. The kits provide high reproducibility, even gene-body coverage, and an accurate representation of GC-rich transcripts.

SMART-Seq mRNA LP includes our patented library preparation kit that incorporates enzymatic fragmentation and stem-loop adapters to construct high-quality, Illumina-compatible libraries from the cDNA synthesized with the core kit. This two-step workflow takes place in a single tube and can be completed in about two hours with no intermediate purification steps necessary—minimizing sample mix-up, sample loss, and cross-contamination. The high library yield generated from this kit provides the flexibility to sequence on high-throughput sequencers while providing high reproducibility. Using the Unique Dual Index kits (Cat. # 634752, 634753, 634754 & 634756; sold separately), up to 384 multiplexed Illumina-ready sequencing libraries can be generated. The kit offers an end-to-end solution from sample to cDNA synthesis, library preparation, and data analysis with our free Cogent NGS bioinformatics pipeline.

If you are interested in these benefits but are working with intact, high-quality single cells, we recommend our SMART-Seq Single Cell PLUS Kit.

If you prefer a random priming approach that will allow you to work with degraded samples and also includes library preparation and indexing reagents, we recommend our SMART-Seq Stranded Kit.

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Cat. # Product Size License Quantity Details
634768 SMART-Seq® mRNA LP 24 Rxns USD $1092.00

License Statement

ID Number  
420 This product is protected by U.S. pending patent and corresponding foreign patents pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
275 SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com.
385 This product is protected by U.S. Patents 7,803,550, 8,399,199, 8,728,737, and 9,598,727 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

SMART-Seq mRNA LP generates oligo(dT)-primed, full-length mRNA-seq libraries. The chemistry is optimized for use on ultra-low amounts of total RNA (10 pg–100 ng, RIN ≥ 8) or for direct use on multiple intact cells (< 1,000 cells). Up to 384 multiplexed, Illumina-ready sequencing libraries can be obtained using the Unique Dual Index kits (Cat. # 634752, 634753, 634754, 634755 & 634756). This kit offers an end-to-end solution including cDNA synthesis, library preparation, and data analysis with our free Cogent NGS bioinformatics tools.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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634768: SMART-Seq mRNA LP

634768: SMART-Seq mRNA LP

Required Products

Cat. # Product Size Price License Quantity Details
634752 Unique Dual Index Kit (1–96) 96 Rxns USD $579.00

The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634753 Unique Dual Index Kit (97–192) 96 Rxns USD $579.00

The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634754 Unique Dual Index Kit (193–288) 96 Rxns USD $579.00

The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634755 Unique Dual Index Kit (289–384) 96 Rxns USD $579.00

The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634756 Unique Dual Index Kit (1–24) 24 Rxns USD $215.00

The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634769 SMART-Seq® mRNA LP 96 Rxns USD $3744.00

License Statement

ID Number  
420 This product is protected by U.S. pending patent and corresponding foreign patents pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
275 SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com.
385 This product is protected by U.S. Patents 7,803,550, 8,399,199, 8,728,737, and 9,598,727 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

SMART-Seq mRNA LP generates oligo(dT)-primed, full-length mRNA-seq libraries. The chemistry is optimized for use on ultra-low amounts of total RNA (10 pg–100 ng, RIN ≥ 8) or for direct use on multiple intact cells (< 1,000 cells). Up to 384 multiplexed, Illumina-ready sequencing libraries can be obtained using the Unique Dual Index kits (Cat. # 634752, 634753, 634754, 634755 & 634756). This kit offers an end-to-end solution including cDNA synthesis, library preparation, and data analysis with our free Cogent NGS bioinformatics tools.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

634769: SMART-Seq mRNA LP

634769: SMART-Seq mRNA LP

Required Products

Cat. # Product Size Price License Quantity Details
634752 Unique Dual Index Kit (1–96) 96 Rxns USD $579.00

The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634753 Unique Dual Index Kit (97–192) 96 Rxns USD $579.00

The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634754 Unique Dual Index Kit (193–288) 96 Rxns USD $579.00

The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634755 Unique Dual Index Kit (289–384) 96 Rxns USD $579.00

The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634756 Unique Dual Index Kit (1–24) 24 Rxns USD $215.00

The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634771 SMART-Seq® mRNA LP 4 x 96 Rxns Inquire for Quotation

License Statement

ID Number  
275 SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com.
420 This product is protected by U.S. pending patent and corresponding foreign patents pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
385 This product is protected by U.S. Patents 7,803,550, 8,399,199, 8,728,737, and 9,598,727 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
*

SMART-Seq mRNA LP generates oligo(dT)-primed, full-length mRNA-seq libraries. The chemistry is optimized for use on ultra-low amounts of total RNA (10 pg–100 ng, RIN≥8) or for direct use on multiple intact cells (<1,000 cells). Up to 384 multiplexed, Illumina-ready sequencing libraries can be obtained using the Unique Dual Index kits (Cat. # 634752–634756). This kit offers an end-to-end solution including cDNA synthesis, library preparation, and data analysis with our free Cogent NGS bioinformatics tools.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

634771: SMART-Seq mRNA LP

634771: SMART-Seq mRNA LP

Required Products

Cat. # Product Size Price License Quantity Details
634752 Unique Dual Index Kit (1–96) 96 Rxns USD $579.00

The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634753 Unique Dual Index Kit (97–192) 96 Rxns USD $579.00

The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634754 Unique Dual Index Kit (193–288) 96 Rxns USD $579.00

The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634755 Unique Dual Index Kit (289–384) 96 Rxns USD $579.00

The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634756 Unique Dual Index Kit (1–24) 24 Rxns USD $215.00

The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634772 SMART-Seq® mRNA 24 Rxns USD $924.00

License Statement

ID Number  
420 This product is protected by U.S. pending patent and corresponding foreign patents pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
275 SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com.

SMART-Seq mRNA generates high-quality oligo(dT)-primed, full-length cDNA from ultra-low amounts of total RNA or directly from multiple intact cells (< 1,000 cells). This kit can accommodate an input volume of 10 μl and is regularly tested with 10 pg of total RNA. The kit is available in 24, 96, or 4 x 96 reaction formats.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

634772: SMART-Seq mRNA

634772: SMART-Seq mRNA
634773 SMART-Seq® mRNA 96 Rxns USD $3168.00

License Statement

ID Number  
420 This product is protected by U.S. pending patent and corresponding foreign patents pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
275 SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com.

SMART-Seq mRNA generates high-quality oligo(dT)-primed, full-length cDNA from ultra-low amounts of total RNA or directly from multiple intact cells (< 1,000 cells). This kit can accommodate an input volume of 10 μl and is regularly tested with 10 pg of total RNA. The kit is available in 24, 96, or 4 x 96 reaction formats.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

634773: SMART-Seq mRNA

634773: SMART-Seq mRNA
634770 SMART-Seq® mRNA 4 x 96 Rxns Inquire for Quotation

License Statement

ID Number  
275 SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com.
420 This product is protected by U.S. pending patent and corresponding foreign patents pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
385 This product is protected by U.S. Patents 7,803,550, 8,399,199, 8,728,737, and 9,598,727 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
*

SMART-Seq mRNA generates high-quality oligo(dT)-primed, full-length cDNA from ultra-low amounts of total RNA or directly from multiple intact cells (<1,000 cells). This kit can accommodate an input volume of 10 μl and is regularly tested with 10 pg of total RNA. The kit is available in 24, 96, or 4 x 96 (384) reaction formats.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

634770: SMART-Seq mRNA

634770: SMART-Seq mRNA

Overview

  • Superior sensitivity and performance for ultra-low inputs—10 pg–100 ng total RNA, or 1–1,000 cells
  • High-quality RNA-seq data—identify the highest number of genes; detect gene fusions, isoforms, SNPs
  • Single-tube cDNA synthesis and library prep workflows—minimized sample loss and handling errors
  • Greater multiplexing—up to 384 libraries with Unique Dual Index kits (Cat. # 634752,  634753, 634754, 634755 & 634756)
  • Complete solution—from sample to data with free, user-friendly Cogent NGS analysis tools

Interested in more data and FAQs about this product? Visit the NGS Learning Center.

More Information

Applications

  • Full-length cDNA synthesis (with SMART-Seq mRNA) and library preparation (with SMART-Seq mRNA LP) from multiple cells or total RNA for transcriptome sequencing
  • cDNA outputs from SMART-Seq mRNA can be used with the SMART-Seq Library Prep kit (sold as part of SMART-Seq mRNA LP) to generate libraries for Illumina sequencing


Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Product Components List to determine kit components. Certificates of Analysis and Product Components Lists are located under the Documents tab.


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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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