ThruPLEX HV chemistry for DNA-seq and tag-seq from FFPE and cell-free DNA
ThruPLEX HV kits are engineered and optimized to generate DNA libraries with high molecular complexity and balanced GC representation from input volumes of 30 µl. This technology provides a complete, fast, and accurate system that enables reliable sequencing from challenging samples. The HV expansion of ThruPLEX technology was designed to accommodate a larger input volume and higher amount of starting material, with protocols now capable of handling inputs of 5 ng to 200 ng of fragmented dsDNA.
ThruPLEX HV kits are engineered and optimized to generate DNA libraries with high molecular complexity and balanced GC representation from input volumes of 30 µl. This technology provides a complete, fast, and accurate system that enables reliable sequencing from challenging samples. The HV expansion of ThruPLEX technology was designed to accommodate a larger input volume and higher amount of starting material, with protocols now capable of handling inputs of 5 ng to 200 ng of fragmented dsDNA. The entire three-step workflow takes place in a single tube or well, in about two hours, with minimal hands-on time. With high library diversity, ThruPLEX HV libraries excel when combined with target enrichment and deliver high-quality sequencing results. Our ThruPLEX DNA-Seq HV and ThruPLEX Tag-Seq HV kits demonstrate robust performance with FFPE and cfDNA, and are offered with pre-plated, single-use UDI indexes for clear sample assignment.1, 2
Unlike other NGS library preparation kits, which are based on ligation of Y-adapters, our patented ThruPLEX HV technology uses stem-loop adapters to construct high-quality libraries in a fast, efficient workflow. This protocol is the simplest in the industry, and enables the addition of adapters containing Illumina-compatible UDIs and UMIs in three short steps. The sample never leaves the tube, ensuring accurate sample tracking, minimizing handling errors, and preventing loss of valuable samples. Sensitive, even coverage is one of the hallmarks of this system, with both DNA-seq and tag-seq kits providing consistent, reproducible coverage for a broad range of GC content, without bias.
ThruPLEX Tag-Seq HV offers added power in correcting for amplification and sequencing errors—key advantages when detecting low-frequency variants. The UMIs included in the system have been carefully selected for even representation and the best possible performance both in error correction and sequencing results on Illumina platforms.
1The ThruPLEX HV UDI kits are not compatible with previous versions of ThruPLEX kits, including the ThruPLEX DNA-seq, ThruPLEX tag-seq, and ThruPLEX Plasma-Seq kits. They are only compatible with the ThruPLEX "HV" kits.
2All indexes have been functionally validated to work with Illumina sequencing systems (e.g., MiSeq®, NovaSeqTM, MiniSeqTM, NextSeq®, and HiSeq® platforms) using two- or four-channel chemistry for base calling. They have not been validated with systems using one-channel chemistry.
Overview
- Streamlined, single-tube workflow without the need to concentrate samples
- Reproducible and uniform library coverage, regardless of input amount
- High-quality data from 5 to 200 ng of FFPE and cfDNA, with input volumes of 30 µl
- Improved performance, without bias, for regions with increasing GC content
- Incorporation of discrete, balanced molecular tags (UMIs) in ThruPLEX Tag-Seq HV
- Sensitive, reliable detection of low-frequency mutations
- Elimination of amplification and sequencing errors
More Information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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