NGS library prep from cfDNA or FFPE DNA—ThruPLEX FLEX
Note: The protocols and QC procedures for ThruPLEX HV kits have been updated to accommodate lower inputs and compatibility with the Unique Dual Index Kit sets. While product naming has been revised accordingly (ThruPLEX FLEX), reagent formulations and product catalog numbers remain unchanged. ThruPLEX HV product bundles (including the ThruPLEX HV UDI and enzymatic fragmentation module) may still be purchased for the time being, but most of these bundles will be phased out and replaced with new ThruPLEX FLEX EF bundles, including enzymatic fragmentation, with UDI kits sold separately.
ThruPLEX FLEX provides a robust, user-friendly NGS library preparation system for processing challenging samples such as cfDNA and FFPE DNA. This technology yields DNA libraries with high molecular complexity and balanced GC representation while accommodating higher inputs (≤200 ng) and input volumes (≤30 µl) than the original ThruPLEX DNA-Seq Kit. All ThruPLEX products feature single-tube workflows that minimize hands-on time, the likelihood of process errors, and the risk of sample loss.
Note: The protocols and QC procedures for ThruPLEX HV kits have been updated to accommodate lower inputs and compatibility with the Unique Dual Index Kit sets. While product naming has been revised accordingly (ThruPLEX FLEX), reagent formulations and product catalog numbers remain unchanged. ThruPLEX HV product bundles (including the ThruPLEX HV UDI and enzymatic fragmentation module) may still be purchased for the time being, but most of these bundles will be phased out and replaced with new ThruPLEX FLEX EF bundles, including enzymatic fragmentation, with UDI kits sold separately.
ThruPLEX FLEX provides a robust, user-friendly NGS library preparation system for processing challenging samples such as cfDNA and FFPE DNA. This technology yields DNA libraries with high molecular complexity and balanced GC representation while accommodating higher inputs (≤200 ng) and input volumes (≤30 µl) than the original ThruPLEX DNA-Seq Kit. All ThruPLEX products feature single-tube workflows that minimize hands-on time, the likelihood of process errors, and the risk of sample loss.
While ThruPLEX DNA-Seq FLEX and ThruPLEX Tag-Seq FLEX kits employ the same core technology, ThruPLEX Tag-Seq FLEX incorporates unique molecular identifiers (UMI) for improved characterization of low-frequency variants. With the patented ThruPLEX approach, leftover adapters are degraded post-ligation, avoiding the need for adapter dilution and intermediate cleanup steps required by other NGS library preparation methods. This approach allows for the most straightforward workflow in the industry, which can be completed in about two hours, with 15 min of hands-on time.
ThruPLEX FLEX technology is unmatched in its ability to provide even coverage across a wide range of GC content with high reproducibility and minimal bias, maximizing library diversity and data quality for genome-wide and targeted sequencing applications.
Overview
- Illumina library construction from 250 pg–200 ng inputs of fragmented dsDNA
- Single-tube workflow saves time and minimizes the likelihood of sample loss
- Superior coverage uniformity and representation of GC-rich regions maximize data quality
- Robust template repair chemistry and flexible input volumes enable efficient processing of challenging samples, including cfDNA and FFPE DNA
- Incorporation of unique molecular identifiers (UMIs) with ThruPLEX Tag-Seq FLEX improves analysis of low-frequency variants
- Compatibility with Unique Dual Index Kit sets allows for multiplexing of up to 384 samples
- Also available with enzymatic fragmentation for processing of intact DNA inputs
More Information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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