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  • ‹ Back to DNA-seq
  • PicoPLEX Gold for single cells
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ThruPLEX HV benchmarking tech note Comparison data: ThruPLEX HV outperforms NEBNext Ultra II
ThruPLEX DNA-seq HV tech note View DNA-seq HV data
ThruPLEX Tag-Seq HV tech note View tag-seq HV data
ThruPLEX Tag-Seq HV UMI instructions Bioinformatics guide: ThruPLEX Tag-Seq HV UMI data analysis
Home › Products › Next-generation sequencing › DNA-seq › DNA-seq for FFPE and cell-free DNA

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ThruPLEX HV benchmarking tech note Comparison data: ThruPLEX HV outperforms NEBNext Ultra II
ThruPLEX DNA-seq HV tech note View DNA-seq HV data
ThruPLEX Tag-Seq HV tech note View tag-seq HV data
ThruPLEX Tag-Seq HV UMI instructions Bioinformatics guide: ThruPLEX Tag-Seq HV UMI data analysis

ThruPLEX HV chemistry for DNA-seq and tag-seq from FFPE and cell-free DNA

ThruPLEX DNA-seq and tag-seq HV kits

ThruPLEX HV is a fast, modular NGS library preparation system engineered and optimized to generate DNA libraries with high molecular complexity and balanced GC representation from input volumes of 30 µl. This technology provides a complete, accurate system that enables reliable sequencing from challenging samples. The HV expansion of ThruPLEX technology accommodates a higher amount of starting material, with protocols now capable of handling inputs of 5 ng to 200 ng of dsDNA.

ThruPLEX HV is a fast, modular NGS library preparation system engineered and optimized to generate DNA libraries with high molecular complexity and balanced GC representation from input volumes of 30 µl. This technology provides a complete, accurate system that enables reliable sequencing from challenging samples. The HV expansion of ThruPLEX technology accommodates a higher amount of starting material, with protocols now capable of handling inputs of 5 ng to 200 ng of dsDNA. The entire three-step workflow takes place in a single tube or well, in about two hours, with minimal hands-on time. With high library diversity, ThruPLEX HV libraries excel when combined with target enrichment and deliver high-quality sequencing results. Our ThruPLEX DNA-Seq HV and ThruPLEX Tag-Seq HV kits demonstrate robust performance with FFPE and cfDNA and are offered with pre-plated, single-use UDI indexes for clear sample assignment.1, 2 

The ThruPLEX HV PLUS kits are the newest members of the ThruPLEX HV family. The ThruPLEX HV PLUS Enzymatic Fragmentation Module is designed to perform size-tunable enzymatic fragmentation in tandem with the ThruPLEX HV repair step. The ThruPLEX DNA-Seq HV PLUS Kit and the ThruPLEX Tag-Seq HV PLUS Kit combine the ThruPLEX HV PLUS Enzymatic Fragmentation Module with the ThruPLEX HV Core Components. The ThruPLEX HV PLUS kits retain the remarkable features of the original ThruPLEX HV system and eliminate any need for mechanical or separate enzymatic fragmentation before ThruPLEX HV library prep.

Unlike other NGS library preparation kits based on ligation of Y-adapters, our patented ThruPLEX HV technology uses stem-loop adapters to construct high-quality libraries in a fast, efficient workflow. This protocol is the simplest in the industry and enables the addition of adapters containing Illumina®-compatible UDIs and UMIs in three short steps. The sample never leaves the tube, ensuring accurate sample tracking, minimizing handling errors, and preventing loss of valuable samples. Sensitive, even coverage is one of the hallmarks of this system, with both DNA-seq and tag-seq kits providing consistent, reproducible coverage for a broad range of GC content, without bias.

ThruPLEX Tag-Seq HV offers added power in correcting for amplification and sequencing errors—key advantages when detecting low-frequency variants. The UMIs included in the system have been carefully selected for even representation and the best possible performance both in error correction and sequencing results on Illumina platforms.

1The ThruPLEX HV UDI kits are not compatible with previous versions of ThruPLEX kits, including the ThruPLEX DNA-Seq, ThruPLEX Tag-Seq, and ThruPLEX Plasma-Seq kits. They are only compatible with the ThruPLEX HV or ThruPLEX HV PLUS kits.

2All indexes have been functionally validated to work with Illumina sequencing systems (e.g., MiSeq®, NovaSeqTM, MiniSeqTM, NextSeq®, and HiSeq® platforms) using two- or four-channel chemistry for base calling. They have not been validated with systems using one-channel chemistry.

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Cat. # Product Size Price License Quantity Details
R400740 ThruPLEX® DNA-Seq HV 96 Rxns USD $2477.00

License Statement

ID Number  
326 This product is protected by U.S. Patents 7,803,550; 8,399,199; 8,728,737, 9,598,727, 10,196,686, 10,208,337 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

ThruPLEX DNA-Seq HV uses a simple, three-step workflow to generate high-complexity DNA libraries from standard samples and challenging sample sources such as FFPE and cell-free plasma DNA. This product contains reagents for 96 reactions and includes unique dual indexing (UDI) primers.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components
R400741 ThruPLEX® DNA-Seq HV 24 Rxns USD $660.00

License Statement

ID Number  
326 This product is protected by U.S. Patents 7,803,550; 8,399,199; 8,728,737, 9,598,727, 10,196,686, 10,208,337 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

ThruPLEX DNA-Seq HV uses a simple, three-step workflow to generate high-complexity DNA libraries from standard samples and challenging sample sources such as FFPE and cell-free plasma DNA. This product contains reagents for 24 reactions and includes unique dual indexing (UDI) primers.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components
R400736 ThruPLEX® DNA-Seq HV Core Components 96 Rxns USD $2112.00

License Statement

ID Number  
326 This product is protected by U.S. Patents 7,803,550; 8,399,199; 8,728,737, 9,598,727, 10,196,686, 10,208,337 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

ThruPLEX DNA-Seq HV Core Components use a simple, three-step workflow to generate high-complexity DNA libraries from standard samples and challenging sample sources such as FFPE and cell-free plasma DNA. Unique dual index (UDI) kits are available for purchase separately. This product contains reagents for 96 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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R400736: ThruPLEX DNA-Seq HV Core Components

R400736: ThruPLEX DNA-Seq HV Core Components
R400737 ThruPLEX® DNA-Seq HV Core Components 24 Rxns USD $588.00

License Statement

ID Number  
326 This product is protected by U.S. Patents 7,803,550; 8,399,199; 8,728,737, 9,598,727, 10,196,686, 10,208,337 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

ThruPLEX DNA-Seq HV Core Components use a simple, three-step workflow to generate high-complexity DNA libraries from standard samples and challenging sample sources such as FFPE and cell-free plasma DNA. Unique dual index (UDI) kits are available for purchase separately. This product contains reagents for 24 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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R400737: ThruPLEX DNA-Seq HV Core Components

R400737: ThruPLEX DNA-Seq HV Core Components
R400782 ThruPLEX® DNA-Seq HV PLUS Kit 24 Rxns USD $818.00

ThruPLEX DNA-Seq HV PLUS integrates the size-tunable ThruPLEX HV PLUS Enzymatic Fragmentation Module into the simple, three-step ThruPLEX HV workflow to generate high-complexity DNA libraries from standard or challenging sample sources such as FFPE and cell-free DNA. This product contains reagents for 24 reactions and includes unique dual index (UDI) primers.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components
R400783 ThruPLEX® DNA-Seq HV PLUS Kit 96 Rxns USD $2912.00

License Statement

ID Number  
326 This product is protected by U.S. Patents 7,803,550; 8,399,199; 8,728,737, 9,598,727, 10,196,686, 10,208,337 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

ThruPLEX DNA-Seq HV PLUS integrates the size-tunable ThruPLEX HV PLUS Enzymatic Fragmentation Module into the simple, three-step ThruPLEX HV workflow to generate high-complexity DNA libraries from standard or challenging sample sources such as FFPE and cell-free DNA. This product contains reagents for 96 reactions and includes unique dual index (UDI) primers.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components
R400739 ThruPLEX® HV UDI 1-24 24 Rxns USD $237.00

ThruPLEX HV UDI 1-24 is a plate-based, single-use set of unique dual indexing primers and contains reagents for processing 24 samples. It is designed for use with ThruPLEX DNA-Seq HV and ThruPLEX Tag-Seq HV kits. This plate is not compatible with ThruPLEX DNA-seq, ThruPLEX tag-seq, ThruPLEX plasma-seq, or PicoPLEX Gold DNA-seq kits.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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R400739: ThruPLEX HV UDI 1-24

R400739: ThruPLEX HV UDI 1-24
R400738 ThruPLEX® HV UDI Set A 96 Rxns USD $986.00

ThruPLEX HV UDI Set A is a plate-based, single-use set of unique dual indexing primers and contains reagents for processing 96 samples. It is designed for use with ThruPLEX DNA-Seq HV and ThruPLEX Tag-Seq HV kits. This plate is not compatible with ThruPLEX DNA-seq, ThruPLEX tag-seq, ThruPLEX plasma-seq, or PicoPLEX Gold DNA-seq kits.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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R400738: ThruPLEX HV UDI Set A

R400738: ThruPLEX HV UDI Set A
R400742 ThruPLEX® Tag-Seq HV 24 Rxns USD $984.00

License Statement

ID Number  
384 This product is protected by U.S. Patents 7,803,550; 8,399,199; 9,598,727, 10,196,686, 10,208,337, and 10,155,942 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

ThruPLEX Tag-Seq HV uses a simple, three-step workflow to generate high-complexity DNA libraries with unique molecular tags from standard samples and challenging sample sources such as FFPE and cell-free plasma DNA. This product contains reagents for 24 reactions and includes unique dual indexing (UDI) primers.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components
R400743 ThruPLEX® Tag-Seq HV 96 Rxns USD $3530.00

License Statement

ID Number  
384 This product is protected by U.S. Patents 7,803,550; 8,399,199; 9,598,727, 10,196,686, 10,208,337, and 10,155,942 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

ThruPLEX Tag-Seq HV uses a simple, three-step workflow to generate high-complexity DNA libraries with unique molecular tags from standard samples and challenging sample sources such as FFPE and cell-free plasma DNA. This product contains reagents for 96 reactions and includes unique dual indexing (UDI) primers.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components
R400735 ThruPLEX® Tag-Seq HV Core Components 96 Rxns USD $3360.00

License Statement

ID Number  
384 This product is protected by U.S. Patents 7,803,550; 8,399,199; 9,598,727, 10,196,686, 10,208,337, and 10,155,942 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

ThruPLEX Tag-Seq HV Core Components use a simple, three-step workflow to generate high-complexity DNA libraries with unique molecular tags from standard samples and challenging sample sources such as FFPE and cell-free plasma DNA. Unique dual index (UDI) kits are available for purchase separately. This product contains reagents for 96 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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R400735: ThruPLEX Tag-Seq HV Core Components

R400735: ThruPLEX Tag-Seq HV Core Components
R400734 ThruPLEX® Tag-Seq HV Core Components 24 Rxns USD $948.00

License Statement

ID Number  
384 This product is protected by U.S. Patents 7,803,550; 8,399,199; 9,598,727, 10,196,686, 10,208,337, and 10,155,942 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

ThruPLEX Tag-Seq HV Core Components use a simple, three-step workflow to generate high-complexity DNA libraries with unique molecular tags from standard samples and challenging sample sources such as FFPE and cell-free plasma DNA. Unique dual index (UDI) kits are available for purchase separately. This product contains reagents for 24 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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R400734: ThruPLEX Tag-Seq HV Core Components

R400734: ThruPLEX Tag-Seq HV Core Components
R400785 ThruPLEX® Tag-Seq HV PLUS Kit 96 Rxns USD $3965.00

License Statement

ID Number  
384 This product is protected by U.S. Patents 7,803,550, 8,399,199; 9,598,727, 10,196,686, 10,208,337, and 10,155,942 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

ThruPLEX Tag-Seq HV PLUS integrates the size-tunable ThruPLEX HV PLUS Enzymatic Fragmentation Module and unique molecular identifiers (UMIs) into the simple, three-step ThruPLEX HV workflow to generate high-complexity DNA libraries from standard or challenging sample sources. With the integration of UMIs, this kit is also suitable for low-frequency mutation detection. This product contains reagents for 96 reactions and includes unique dual index (UDI) primers.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components
R400784 ThruPLEX® Tag-Seq HV PLUS Kit 24 Rxns USD $1142.00

License Statement

ID Number  
384 This product is protected by U.S. Patents 7,803,550, 8,399,199; 9,598,727, 10,196,686, 10,208,337, and 10,155,942 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

ThruPLEX Tag-Seq HV PLUS integrates the size-tunable ThruPLEX HV PLUS Enzymatic Fragmentation Module and unique molecular identifiers (UMIs) into the simple, three-step ThruPLEX HV workflow to generate high-complexity DNA libraries from standard or challenging sample sources. With the integration of UMIs, this kit is also suitable for low-frequency mutation detection. This product contains reagents for 24 reactions and includes unique dual index (UDI) primers.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components

Blue and purple with NGS on it Improvements with ThruPLEX HV PLUS
Comparing ThruPLEX HV PLUS to competitors

Overview

  • Streamlined, single-tube workflow without the need to concentrate samples
  • Size-tunable enzymatic fragmentation module
  • Reproducible and uniform library coverage, regardless of input amount
  • High-quality data from 5 to 200 ng of FFPE and cfDNA, with input volumes of 30 µl
  • Improved performance, without bias, for regions with increasing GC content
  • Incorporation of discrete, balanced molecular tags (UMIs) in ThruPLEX Tag-Seq HV and ThruPLEX Tag-Seq HV PLUS
    • Sensitive, reliable detection of low-frequency mutations
    • Elimination of amplification and sequencing errors

More Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2021 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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The rapid spread of severe infections by viruses such as SARS-CoV-2, HIV, H1N1, Ebola, and Zika has highlighted the critical need for the rapid development of vaccines against previously unknown pathogens to deal with pandemics such as COVID-19 effectively.

Takara Bio is proud to be on the front line in the fight to defeat the novel coronavirus by enabling innovative vaccine development. This section discusses tools and techniques to overcome the challenges faced during the vaccine development process.

Learn how our products help speed up vaccine development

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Capturem Trypsin for a rapid, efficient mass spectometry workflow at room temperature.

Speed up your mass spec workflow

Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis. This product utilizes our novel Capturem technology in a spin column format with membrane-immobilized trypsin. Capturem Trypsin Columns may be used to completely digest protein samples in less than a minute with digestion efficiencies (protein coverage) comparable to or better than those obtained using in-solution trypsin digestion.

Capturem trypsin technology

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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