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PrimeCap T7 RNA Polymerase (low dsRNA)

Trace amounts of double-stranded RNA (dsRNA) byproduct produced during in vitro transcription can interfere with downstream in vivo administration by triggering unwanted immune responses. PrimeCap T7 RNA Polymerase (low dsRNA) is a modified form of T7 RNA polymerase that combines highly efficient cap-analog-dependent RNA synthesis with extremely low dsRNA generation, making it the ideal choice for researchers engaged in mRNA therapeutic research and development.

Cat. # Product Size Price License Quantity Details
2560A PrimeCap™ T7 RNA Polymerase (low dsRNA) 20,000 U USD $271.00

PrimeCap T7 RNA Polymerase (low dsRNA) is a DNA-dependent RNA polymerase that produces high yields of capped, in vitro-transcribed RNA with extremely low dsRNA byproduct. PrimeCap T7 RNA Polymerase (200 U/μl) and 10X T7 RNA Polymerase Buffer are included.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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2561A PrimeCap™ T7 RNA Polymerase (low dsRNA), HQ 200,000 U Inquire for Quotation *

PrimeCap T7 RNA Polymerase (low dsRNA), HQ is a DNA-dependent RNA polymerase that produces high yields of capped, in vitro-transcribed RNA with extremely low dsRNA byproduct. PrimeCap T7 RNA Polymerase (200 U/μl) and 10X T7 RNA Polymerase Buffer are included.

PrimeCap T7 RNA Polymerase, HQ (high quality) has been manufactured with stricter quality standards than Cat. # 2560A and is supplied in a higher volume for your scale-up process. It is free from human- or animal-derived materials, as well as free from β-lactam compounds in the final formulation.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components
T2422S001 PrimeCap™ T7 RNA Polymerase (low dsRNA) GMP grade 200,000 U Inquire for Quotation *

PrimeCap T7 RNA Polymerase (low dsRNA) GMP grade is a DNA-dependent RNA polymerase that produces high yields of capped, in vitro-transcribed RNA with extremely low dsRNA byproduct. This is the enzyme only; 10X IVT Reaction Buffer (Cat. # T2401S010) is sold separately. 10 ml- and 100 ml-sized versions of this product are available by request. Reach out to your local sales team.

This product is manufactured in accordance with relevant GMP guidelines. No animal- or human- derived components are used in the manufacture of this product. This product uses primary materials that do not contain β-lactam compounds. Please see the Certificate of Analysis (COA) for each lot.

Notice to purchaser

This product is to be used for research use only, including non-commercial manufacturing for clinical research. This product is not intended for humans or animals in-vivo applications. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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T2401S010 10X IVT Reaction Buffer GMP grade 10 ml Inquire for Quotation *

Reaction buffer for use with PrimeCap T7 RNA Polymerase (low dsRNA) GMP grade to generate a large quantity of high-quality RNA.

Notice to purchaser

This product is to be used for research use only, including non-commercial manufacturing for clinical research. This product is not intended for humans or animals in-vivo applications. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components

*You must be logged in to a Purchasing Account in order to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@takarabio.com.

Overview

  • Produce in-vitro transcribed RNA with very low dsRNA byproduct that is notably safer for in vivo administration
  • Retain the same high yields as T7 RNA polymerase ver.2 (Cat. # 2541A), especially when used with cap analogs such as CleanCap Reagent AG (TriLink Bio Technologies)
  • Get improved heat resistance (up to 52°C) compared to T7 RNA polymerase ver.2.0

Produce mRNA with very low dsRNA byproduct—without sacrificing yield

T7 RNA Polymerase ver.2.0 (Cat. #2541A) and PrimeCap T7 RNA Polymerase were used to synthesize mRNA from the Positive Control Template (FLuc) plasmid (sold as part of Cat. #6144). CleanCap Reagent AG (4 mM; TriLink BioTechnologies) and Pyrophosphatase (Cat. #2450A/B) were added to the in vitro transcription reaction. Panel A. mRNA yields of using T7 RNA Polymerase ver.2.0 or PrimeCap T7 RNA Polymerase in a 20 µl reaction volume. Panel B. Percentage of dsRNA per reaction as compared to T7 RNA Polymerase ver.2.0 (WT on y-axis), as determined by a dsRNA ELISA kit.

More Information

Protocol

RNase-free Water up to 20 μl
10X T7 RNA Polymerase Buffer 2 μl
ATP, CTP, GTP, UTP each 10 mM
Template DNA 0.5–2 μg
Recombinant RNase Inhibitor ver.2.0 20 U
Pyrophosphatase (inorganic) 0.1 U
PrimeCap T7 RNA Polymerase 200 U

Incubate at 37°C for 1–2 hr.

Applications

  • Synthesis of capped RNA using a cap analog
  • Synthesis of uncapped RNA for enzymatic capping (note: RNA yield will be 10–40% lower than when using cap analogs)

Source

Escherichia coli carrying a plasmid containing the gene for the modified phage T7 RNA polymerase variant.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Takara IVTpro mRNA Synthesis System

A complete solution for synthesizing high-quality single-stranded mRNA containing your gene of interest with a cap structure and poly(A) sequence.

Oxidation-resistant Recombinant RNase Inhibitor

The oxidation-resistant Recombinant RNase Inhibitor ver.2.0 inhibits RNAse A and is suitable for use in enzymatic reactions requiring DTT concentrations, such as in vitro transcription, in vitro translation, and RT-PCR.

Inorganic Pyrophosphate

Use our inorganic pyrophosphatase to improve the RNA yield in in vitro transcription reactions.

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