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Cellartis iPS Cell to Hepatocyte Differentiation System

iPS cell to hepatocyte differentiation system

Hepatocytes derived from human induced pluripotent stem (hiPS) cells using the Cellartis iPS Cell to Hepatocyte Differentiation System are an alternative to primary hepatocytes, as they exhibit sufficient expression levels of drug-metabolizing enzymes and transporters and demonstrate stable functionality over time in culture. In addition, hiPS cell-derived hepatocytes can provide an accurate reflection of the metabolic diversity observed in the human population.

Hepatocytes derived from human induced pluripotent stem (hiPS) cells using the Cellartis iPS Cell to Hepatocyte Differentiation System are an alternative to primary hepatocytes, as they exhibit sufficient expression levels of drug-metabolizing enzymes and transporters and demonstrate stable functionality over time in culture. In addition, hiPS cell-derived hepatocytes can provide an accurate reflection of the metabolic diversity observed in the human population. The Cellartis iPS Cell to Hepatocyte Differentiation System provides a complete solution for generating functional, hiPS cell-derived hepatocytes within three weeks.

The process of generating hiPS cell-derived hepatocytes begins with the directed differentiation of hiPS cells into Definitive Endoderm (DE) cells that are then differentiated further into hepatocytes. The complete system provides media and ready-to-use coatings for each step of the iPS-cell-to-hepatocyte differentiation protocol. Starting with approximately 3 x 106 undifferentiated hiPS cells, this system yields 5 x 106 hepatocytes, equivalent to a confluent monolayer of 50 cm2. This do-it-yourself system offers a solution for the consistent production of assay-ready cells from patient-derived cells or from Cellartis brand iPS cell lines—enabling highly reproducible results.

Hepatocytes obtained with the Cellartis iPS Cell to Hepatocyte Differentiation System express major hepatic markers and show relevant CYP enzyme activities based on the genetic background of the original hiPS cells from which they were derived. In addition, these freshly differentiated hepatocytes are functionally stable for a longer timeframe compared to cryopreserved primary hepatocytes, enabling prolonged experiments such as long-term toxicity assays or viral infection studies. Cellartis Hepatocyte Maintenance Medium (Cat. # Y30051) is recommended for long-term maintenance of hiPS cell-derived hepatocytes.

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Cat. # Product Size License Quantity Details
Y30055 Cellartis® iPS Cell to Hepatocyte Differentiation System 1 Kit USD $1380.00

License Statement

ID Number  
C007 This product is manufactured and sold by Takara Bio Inc. and/or its affiliate(s) (“Takara”) under a license from ViaCyte Inc. (“ViaCyte”). This product is covered by one or more claims of patents owned by ViaCyte, including WO2005063971, WO2006017134, WO2006071911, WO2005116073 and WO200616999, and their foreign counterparts. The use of this product is strictly limited to purchaser's own internal research. Purchaser has no right to use this product or its components in humans for any purposes including but not limited to diagnostics, therapeutics, or clinical trials. Purchaser has no right to resell or transfer this product to a third party regardless of whether or not compensation is received. Purchasers wishing to use this product for purposes other than internal research use should contact Takara. No express or implied license is granted to the purchaser.
C001 This product is manufactured and sold by Takara Bio Europe AB based on a commercial license to certain intellectual property rights held by Wisconsin Alumni Research Foundation (“WARF”). This product is covered by one or more claims of U.S. Patent No. 7,514,260 and its foreign counterparts. The purchase of this product conveys to the buyer the non-transferable right to use the product for its intended use, strictly limited to purchaser’s own internal research. No other express or implied license is granted to the purchaser. Purchaser cannot have any right to use this product or its components in humans for any purposes including but not limited to diagnostics and/or therapeutics, or otherwise clinical trials. Purchase does not include any right to resell or transfer this product to a third party regardless of whether or not compensation is received. Purchasers wishing to use this product for purposes other than internal research use should contact us.

The Cellartis iPS Cell to Hepatocyte Differentiation System is a complete system for differentiation of human induced pluripotent stem (iPS) cells to hepatocytes via definitive endoderm (DE). This kit includes the Cellartis DEF-CS Culture System for expansion of undifferentiated iPS cells, the Cellartis Definitive Endoderm Differentiation Kit for differentiation to DE cells, and the Cellartis Hepatocyte Differentiation Kit for subsequent differentiation to hepatocytes.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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Immunocytochemistry analysis of hepatocyte differentiation

Immunocytochemistry analysis of hepatocyte differentiation
Immunocytochemistry analysis of hepatocyte differentiation. hiPS cells were differentiated into functional hepatocytes using the Cellartis iPS Cell to Hepatocyte Differentiation System. Hepatocytes were immunostained to detect early hepatic markers HNF4α (red, nuclear) and CK18 (green) on days 14 and 21. As the hepatocytes matured, expression of liver-specific markers CYP3A (red, cytoplasmic) and Albumin (green) increased as seen on day 28, as expected. Cell nuclei were stained with DAPI (blue).

Required Products

Cat. # Product Size Price License Quantity Details
Y30051 Cellartis® Hepatocyte Maintenance Medium 100 mL USD $279.00

Cellartis Hepatocyte Maintenance Medium are used for prolonged maintenance of hepatocyte cells in 2D culture, derived using Cellartis Hepatocyte Differentiation Kit (Cat. No. Y30050) or Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055).

Documents Components
Y30051 Cellartis® Hepatocyte Maintenance Medium 100 mL USD $279.00

Cellartis Hepatocyte Maintenance Medium are used for prolonged maintenance of hepatocyte cells in 2D culture, derived using Cellartis Hepatocyte Differentiation Kit (Cat. No. Y30050) or Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components

Overview

  • Highly reproducible, robust system—the exact same protocol has been shown to work across 25 different iPS cell lines, so there's no need to optimize for your lines

  • Ideal for drug metabolism and safety studies—consistently generate panels of >90% pure, functional, hiPS cell-derived hepatocytes with diverse genetic backgrounds

  • Customized starting materials—start with any disease-relevant iPS cell lines and create accurate liver disease models

  • Ready for personalized medicine—make patient-specific, disease-specific cells for therapeutic applications 

  • Extended experimental window—hepatocytes can be used for a minimum of 11 days for functional tests

More Information

Components

  • Cellartis DEF-CS 100 Culture System (Cat. # Y30020, not sold separately)

  • Cellartis Definitive Endoderm Differentiation Kit (Cat. # Y30030, not sold separately)

  • Cellartis Hepatocyte Differentiation Kit (Cat. # Y30050)

Product citations

Asplund, A. et al. One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells. Stem Cell Rev. Reports 12, 90–104 (2016).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


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