Cellartis DEF-CS 500 Culture System
The Cellartis DEF-CS 500 Culture System is an easy-to-use, complete culture system for defined and feeder-free ES and iPS cell expansion in a non-colony, 2D-monolayer format.
The Cellartis DEF-CS 500 Culture System is an easy-to-use, complete culture system for defined and feeder-free ES and iPS cell expansion in a non-colony, 2D-monolayer format.
With this system, cells are maintained in an undifferentiated state with virtually no background differentiation, eliminating the need for cell selection. The highly reproducible nature of the system, coupled with its ability to ensure an efficient and predictable growth rate, makes the DEF-CS culture system ideal for the expansion and scale-up of a homogeneous population of iPS cells. Following reprogramming, successful expansion of your pluripotent stem cells is crucial. The DEF-CS culture system, a research-grade system, provides media, a coating reagent, and growth factors for the easy culture of iPS cells as a 2D monolayer.
Overview
- Non-colony, 2D-monolayer format enables you to culture pluripotent stem cells just like any other cell type
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Feeder-free and chemically defined
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Cells maintain pluripotency and long-term potential for self-renewal and differentiation
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Robust system provides high reproducibility, stable growth, and ensures maintenance of a normal karyotype
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Complete kit includes 500 ml of basal medium, additives, and coating compound
More Information
Applications
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Single-cell culture of human iPS cells
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Transfection and reprogramming
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High-throughput screening
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Tissue engineering (seeding cells on a scaffold)
- Gene editing
Product citations
Boreström, C. et al. Footprint-free human induced pluripotent stem cells from articular cartilage with redifferentiation capacity: A first step toward a clinical-grade cell source. Stem Cells Trans. Med. 3, 433–447 (2014).
Hanson, C. et al. Transplantation of human embryonic stem cells onto a partially wounded human cornea in vitro. Acta ophthalmologica 91, 127–130 (2013).
Kia, R. et al. MicroRNA-122: a novel hepatocyte-enriched in vitro marker of drug-induced cellular toxicity. Toxicol. Sci. 144, 173–85 (2015).
Norrman, K. et al. Distinct gene expression signatures in human embryonic stem cells differentiated towards definitive endoderm at single-cell level. Methods 59, 59–70 (2013).
Sivertsson, L. et al. Hepatic differentiation and maturation of human embryonic stem cells cultured in a perfused three-dimensional bioreactor. Stem Cells Dev. 22, 581–594 (2012).
Ulvestad, M. et al. Drug metabolizing enzyme and transporter protein profiles of hepatocytes derived from human embryonic and induced pluripotent stem cells. Biochem. Pharmacol. 86, 691–702 (2013).
Valton, J. et al. Efficient strategies for TALEN-mediated genome editing in mammalian cell lines. Methods 69, 151–170 (2014).
Zandén, C. et al. Stem cell responses to plasma surface modified electrospun polyurethane scaffolds. NANOMED-NBM 10, 949–958 (2014).
Additional product information
Please see the product′s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
More applications of the DEF-CS 500 Culture System
Using the DEF-CS system to culture human iPS cells
Learn how human pluripotent stem cells are cultured in the DEF-CS culture system.
Reprogramming PBMCs
View data on reprogramming PBMCs to iPSCs with a high success rate.
Reprogramming fibroblasts
View data on reprogramming fibroblasts to iPSCs with a high success rate.
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