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  • Cellartis DEF-CS 500 Culture System
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Comparison of the Cellartis DEF-CS system with stem cell media from different vendors DEF-CS vs. the competitors
Gene editing in hiPS cells Gene editing in hiPS
Liver organoid differentiation from iPSCs (ChiPSC18) using the Cellartis DEF-CS 500 Culture System for prediction of drug-induced liver injury Liver organoid differentiation from iPSCs
Home › Products › Stem cell research › Media, differentiation kits, and matrices › Pluripotent stem cell media › Cellartis DEF-CS 500 Culture System

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Comparison of the Cellartis DEF-CS system with stem cell media from different vendors DEF-CS vs. the competitors
Gene editing in hiPS cells Gene editing in hiPS
Liver organoid differentiation from iPSCs (ChiPSC18) using the Cellartis DEF-CS 500 Culture System for prediction of drug-induced liver injury Liver organoid differentiation from iPSCs

Cellartis DEF-CS 500 Culture System

Human iPS cells in DEF-CS

The Cellartis DEF-CS 500 Culture System is an easy-to-use, complete culture system for defined and feeder-free ES and iPS cell expansion in a non-colony, 2D-monolayer format.

The Cellartis DEF-CS 500 Culture System is an easy-to-use, complete culture system for defined and feeder-free ES and iPS cell expansion in a non-colony, 2D-monolayer format.

With this system, cells are maintained in an undifferentiated state with virtually no background differentiation, eliminating the need for cell selection. The highly reproducible nature of the system, coupled with its ability to ensure an efficient and predictable growth rate, makes the DEF-CS culture system ideal for the expansion and scale-up of a homogeneous population of iPS cells. Following reprogramming, successful expansion of your pluripotent stem cells is crucial. The DEF-CS culture system, a research-grade system, provides media, a coating reagent, and growth factors for the easy culture of iPS cells as a 2D monolayer.

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Cat. # Product Size Price License Quantity Details
Y30012 Cellartis® DEF-CS™ 500 COAT-1 4 mL USD $282.00

Cellartis DEF-CS 500 COAT-1 is a part of the Cellartis DEF-CS 500 Culture System, a defined culture system for efficient expansion of undifferentiated human pluripotent stem cells. In addition to the coating substrate, the complete System includes basal medium and additives.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Y30012: Cellartis DEF-CS 500 COAT-1

Y30012: Cellartis DEF-CS 500 COAT-1
Y30010 Cellartis® DEF-CS™ 500 Culture System 1 Kit USD $583.00

License Statement

ID Number  
C001 This product is manufactured and sold by Takara Bio Europe SAS based on a commercial license to certain intellectual property rights held by Wisconsin Alumni Research Foundation (“WARF”). This product is covered by one or more claims of U.S. Patent No. 7,514,260 and its foreign counterparts. The purchase of this product conveys to the buyer the non-transferable right to use the product for its intended use, strictly limited to purchaser’s own internal research. No other express or implied license is granted to the purchaser. Purchaser cannot have any right to use this product or its components in humans for any purposes including but not limited to diagnostics and/or therapeutics, or otherwise clinical trials. Purchase does not include any right to resell or transfer this product to a third party regardless of whether or not compensation is received. Purchasers wishing to use this product for purposes other than internal research use should contact us.

Cellartis DEF-CS 500 Culture System is a defined culture system for efficient expansion of undifferentiated human pluripotent stem cells. This kit includes basal medium, coating substrate, and additives.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Expansion potential of a characterized working bank of human induced pluripotent stem (iPS) cells in the Cellartis DEF-CS Culture System

Expansion potential of a characterized working bank of human induced pluripotent stem (iPS) cells in the Cellartis DEF-CS Culture System
Expansion potential of a characterized working bank of human induced pluripotent stem (iPS) cells in the Cellartis DEF-CS Culture System. The Cellartis DEF-CS Culture System can produce 2 x 109 human iPS cells within 4 passages (18–20 days) from frozen cells (2.0–2.5 x 106 cells).

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Robust growth of human induced pluripotent stem (iPS) cells in the Cellartis DEF-CS Culture System

Robust growth of human induced pluripotent stem (iPS) cells in the Cellartis DEF-CS Culture System
Robust growth of human induced pluripotent stem (iPS) cells in the Cellartis DEF-CS Culture System. The number of iPS cells was quantified after being cultured for three weeks using either the Cellartis DEF-CS Culture System, a reference feeder system, or four other stem cell culture systems.

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Human induced pluripotent stem cells (iPS) cells grown in the Cellartis DEF-CS Culture System have the highest proportion and intensity of markers of pluripotency

Human induced pluripotent stem cells (iPS) cells grown in the Cellartis DEF-CS Culture System have the highest proportion and intensity of markers of pluripotency
Human induced pluripotent stem cells (iPS) cells grown in the Cellartis DEF-CS Culture System have the highest proportion and intensity of markers of pluripotency. Quantitative analysis of TRA1-60 (Panel A) and SSEA4 (Panel B) expression was performed on human iPS cells after five weeks culture in either the Cellartis DEF-CS Culture System, a reference feeder cell containing system, or four different stem cell culture systems.

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Human iPS cells grown in the Cellartis DEF-CS Culture System look different from those grown with traditional aggregate culture techniques

Human iPS cells grown in the Cellartis DEF-CS Culture System look different from those grown with traditional aggregate culture techniques
Human iPS cells grown in the Cellartis DEF-CS Culture System look different from those grown with traditional aggregate culture techniques. Freshly passaged human iPS cells were cultured for 5 days in either the Cellartis DEF-CS Culture System, on feeder cells, in mTeSR 1 medium (STEMCELL Technologies), or in Essential 8 Medium (E8; Life Technologies).

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Human induced pluripotent stem (iPS) cells cultured long-term in the Cellartis DEF-CS Culture System retain a normal karyotype

Human induced pluripotent stem (iPS) cells cultured long-term in the Cellartis DEF-CS Culture System retain a normal karyotype
Human induced pluripotent stem (iPS) cells cultured long-term in the Cellartis DEF-CS Culture System retain a normal karyotype. The human iPS cell line ChiPSC18 was cultured for 20 passages in the Cellartis DEF-CS Culture System. Chromosomal analysis indicates that the cells retain a normal karyotype.

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Human induced pluripotent stem (iPS) cells can be passaged as single cells in the Cellartis DEF-CS Culture System

Human induced pluripotent stem (iPS) cells can be passaged as single cells in the Cellartis DEF-CS Culture System

Human induced pluripotent stem (iPS) cells can be passaged as single cells in the Cellartis DEF-CS Culture System. A single GFP-actin iPS cell was isolated and placed in the well of a culture dish. Twenty-four hours after seeding, morphology was assessed by fluorescence microscopy at 20x (Panel A) and 40x (Panel B) magnification. Sixteen days later, the single GFP-actin iPS cell had proliferated into numerous cells as evidenced by microscopic observation at 4x (Panel C), 10x (Panel D), 20x (Panel E), and 40x (Panel F) magnification.

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Human pluripotent stem cells remain undifferentiated when cultured in the Cellartis DEF-CS Culture System

Human pluripotent stem cells remain undifferentiated when cultured in the Cellartis DEF-CS Culture System

Human pluripotent stem cells remain undifferentiated when cultured in the Cellartis DEF-CS Culture System. Human iPS cells cultured for 23 passages in the Cellartis DEF-CS Culture System were characterized by Oct-4 staining (Panel A) and nuclear staining (Panel B).

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Y30010: Cellartis DEF-CS 500 Culture System

Y30010: Cellartis DEF-CS 500 Culture System
Y30017 Cellartis® DEF-CS™ 500 Basal Medium with Additives 500 mL USD $478.00

License Statement

ID Number  
C001 This product is manufactured and sold by Takara Bio Europe SAS based on a commercial license to certain intellectual property rights held by Wisconsin Alumni Research Foundation (“WARF”). This product is covered by one or more claims of U.S. Patent No. 7,514,260 and its foreign counterparts. The purchase of this product conveys to the buyer the non-transferable right to use the product for its intended use, strictly limited to purchaser’s own internal research. No other express or implied license is granted to the purchaser. Purchaser cannot have any right to use this product or its components in humans for any purposes including but not limited to diagnostics and/or therapeutics, or otherwise clinical trials. Purchase does not include any right to resell or transfer this product to a third party regardless of whether or not compensation is received. Purchasers wishing to use this product for purposes other than internal research use should contact us.

Cellartis DEF-CS 500 Basal Medium with Additives is a robust media for efficient expansion of human induced pluripotent stem (iPS) cells in a feeder-free and defined environment. This kit includes Cellartis DEF-CS 500 Basal Medium and Cellartis DEF-CS 500 Additives.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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Y30017: Cellartis DEF-CS 500 Basal Medium with Additives

Y30017: Cellartis DEF-CS 500 Basal Medium with Additives

Cellartis Human iPS Cell Line Kits contain human induced pluripotent stem (iPS) cells along with a starter set for the Cellartis DEF-CS Culture System. Human pluripotent stem cells
Cellartis single-cell cloning protocol
Cellartis human ES cell line kits contain human embryonic stem (ES) cells along with a starter set for the Cellartis DEF-CS culture system. Human embryonic stem cells

Overview

  • Non-colony, 2D-monolayer format enables you to culture pluripotent stem cells just like any other cell type
  • Feeder-free and chemically defined 

  • Cells maintain pluripotency and long-term potential for self-renewal and differentiation

  • Robust system provides high reproducibility, stable growth, and ensures maintenance of a normal karyotype

  • Complete kit includes 500 ml of basal medium, additives, and coating compound

More Information

Applications

  • Single-cell culture of human iPS cells

  • Transfection and reprogramming

  • High-throughput screening

  • Tissue engineering (seeding cells on a scaffold)

  • Gene editing

Product citations

Boreström, C. et al. Footprint-free human induced pluripotent stem cells from articular cartilage with redifferentiation capacity: A first step toward a clinical-grade cell source. Stem Cells Trans. Med. 3, 433–447 (2014).

Hanson, C. et al. Transplantation of human embryonic stem cells onto a partially wounded human cornea in vitro. Acta ophthalmologica 91, 127–130 (2013).

Kia, R. et al. MicroRNA-122: a novel hepatocyte-enriched in vitro marker of drug-induced cellular toxicity. Toxicol. Sci. 144, 173–85 (2015).

Norrman, K. et al. Distinct gene expression signatures in human embryonic stem cells differentiated towards definitive endoderm at single-cell level. Methods 59, 59–70 (2013).

Sivertsson, L. et al. Hepatic differentiation and maturation of human embryonic stem cells cultured in a perfused three-dimensional bioreactor. Stem Cells Dev. 22, 581–594 (2012).

Ulvestad, M. et al. Drug metabolizing enzyme and transporter protein profiles of hepatocytes derived from human embryonic and induced pluripotent stem cells. Biochem. Pharmacol. 86, 691–702 (2013).

Valton, J. et al. Efficient strategies for TALEN-mediated genome editing in mammalian cell lines. Methods 69, 151–170 (2014).

Zandén, C. et al. Stem cell responses to plasma surface modified electrospun polyurethane scaffolds. NANOMED-NBM 10, 949–958 (2014).

Additional product information

Please see the product′s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


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More applications of the DEF-CS 500 Culture System

Using the DEF-CS system to culture human iPS cells

Learn how human pluripotent stem cells are cultured in the DEF-CS culture system.

Reprogramming PBMCs

View data on reprogramming PBMCs to iPSCs with a high success rate.

Reprogramming fibroblasts

View data on reprogramming fibroblasts to iPSCs with a high success rate.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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