Premix Ex Taq master mix for probe-based real-time PCR
Premix Ex Taq (Probe qPCR) is a 2X master mix for real-time PCR (qPCR) using probe-based qPCR or 5' nuclease assays. This 2X master mix includes Takara Ex Taq HS—a hot-start PCR enzyme in combination with anti-Taq antibody—in a qPCR-optimized buffer.Takara Ex Taq HS inhibits non-specific amplification while enabling high-efficiency amplification and detection sensitivity during real-time PCR analyses. Additionally, Tli RNase H, a heat-resistant RNase enzyme, is included in the real-time PCR premix in order to minimize PCR inhibition due to the presence of residual mRNA in the input cDNA. This master mix is ideal for high-speed PCR, allows accurate target quantification and detection over a broad dynamic range, and enables highly reproducible and reliable real-time PCR analyses.
Rapid and accurate PCR product detection
Quantitative gene expression analysis using real-time PCR (qPCR)
Included Tli RNase H minimizes PCR inhibition by removing residual mRNA in input cDNA
Real-time PCR (qPCR) quantification of DNA using methods such as probe-based qPCR assays and 5' nuclease assays
Two tubes of ROX Reference Dye are supplied separately with Cat. # RR390A/B and RR390L/W. The ROX Dye serves as a convenient internal reference standard when normalizing signals resulting from non-PCR fluorescence fluctuations that occur between wells or over time in different instruments.
Cat. # RR390L/W are larger package sizes—5 ml and 25 ml (5 x 5 ml), respectively—of Premix Ex Taq (Probe qPCR). Each of these items provides convenient larger packaging and is supplied with a single, separate vial of ROX Reference Dye.
Cat. # RR39LR/WR are larger package sizes—5 ml and 25 ml (5 x 5 ml), respectively—that already contain ROX Reference Dye in the real-time PCR Premix.
Ishizaka, A. et al. Double plant homeodomain (PHD) finger proteins DPF3a and-3b are required as transcriptional co-activators in SWI/SNF complex-dependent activation of NF-κB. J. Biol. Chem.287:11924–11933 (2012).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.