5X PrimePath Probe qPCR Kit, GPR

The 5X PrimePath Probe qPCR Kit, GPR is ideal for sensitive pathogen detection via probe-based qPCR (using the 5′ nuclease method). The increased, 5X concentration of the master mix frees up reaction space in your qPCR-based pathogen detection assays; this allows you to add more of your sample, which improves the limit of detection, or to add additional primers/probes to develop multiplex assays.

The kit contains our efficient and highly specific hot-start PCR enzyme, TaKaRa Taq HS, in an optimized reaction buffer. Simply add a DNA sample, primers, and probes to the master mix to get the reaction started. The entire protocol, from setup to detection, takes less than one hour.

The 5X PrimePath Probe qPCR Kit, GPR is a general purpose reagent (GPR) labeled “For Laboratory Use” and is manufactured in a facility certified to ISO 13485. Every lot is functionally tested to ensure lot-to-lot reproducibility and consistent qPCR performance across a large dynamic range.

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Cat. #	Product	Size
638349	5X PrimePath™ Probe qPCR Kit, no ROX, GPR	200 Rxns	USD $333.00
The 5X PrimePath Probe qPCR Kit, no ROX, GPR is a real-time PCR master mix containing TaKaRa Taq HS premixed with the buffers needed for probe-based qPCR using DNA samples. The 5X concentration of the master mix allows for increased sample volumes to improve sensitivity or the ability to add additional primer/probes for the development of multiplex assays. Cat. # 638349 contains reagents sufficient to perform 200 x 25 µl reactions. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Dynamic range of 5X PrimePath Probe qPCR Kit, no ROX, GPR Dynamic range of 5X PrimePath Probe qPCR Kit, no ROX, GPR. Human cDNA was amplified using human GAPDH primers/probes with 5X PrimePath Probe qPCR Kit, no ROX plus, GPR on a CFX96 Real-Time PCR System. Two replicates of a sixfold dilution series of human cDNA were tested. The amplification plot for the dilution series is shown. Back Lot-to-lot consistency of 5X PrimePath Probe qPCR Kit, no ROX, GPR Lot-to-lot consistency of 5X PrimePath Probe qPCR Kit, no ROX, GPR. Three separate lots of 5X PrimePath Probe qPCR Kit, no ROX, GPR were used to amplify 100 ng, 10 ng, 1 ng, 100 pg, or 10 pg of human cDNA using a human GAPDH primer/probe set. The Cq for each amount of human cDNA using Lot 1, Lot 2, or Lot 3 of 5X PrimePath Probe qPCR Kit, no ROX, GPR are shown
638350	5X PrimePath™ Probe qPCR Kit, ROX plus, GPR	200 Rxns	USD $333.00
The 5X PrimePath Probe qPCR Kit, ROX plus, GPR is a real-time PCR master mix containing TaKaRa Taq HS premixed with ROX dye and the buffers needed for probe-based qPCR using DNA samples. The 5X concentration of the master mix allows for increased sample volumes to improve sensitivity or the ability to add additional primer/probes for the development of multiplex assays. Cat. # 638350 contains reagents sufficient to perform 200 x 25 µl reactions. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Dynamic range of 5X Primepath Probe qPCR Kit, ROX plus, GPR Dynamic range of 5X Primepath Probe qPCR Kit, ROX plus, GPR. Human cDNA was amplified using human GAPDH primers/probes with 5X PrimePath Probe qPCR Kit, ROX plus, GPR on a QuantStudio Real-Time PCR System. Two replicates of a sixfold dilution series consisting of 100 ng (blue), 10 ng (green), 1 ng (orange), 100 pg (gray), 10 pg (red), and 1 pg (purple) of human cDNA and a no-template control (black) were tested. The amplification plot for the dilution series is shown. Back Dynamic range of 5X PrimePath Probe qPCR Kit, ROX plus, GPR using three different Mpox primer/probe sets Dynamic range of 5X PrimePath Probe qPCR Kit, ROX plus, GPR using three different Mpox primer/probe sets. Mpox-positive control DNA was amplified using (Panel A) a generic Mpox primer/probe set, (Panel B) a West African strain primer/probe set, or a (Panel C) Congo Basin strain primer/probe set with 5X PrimePath Probe qPCR Kit, ROX plus, GPR on a QuantStudio Real-Time PCR System. Two replicates of a fivefold dilution series consisting of 50,000 copies (red), 5,000 copies (blue), 500 copies (green), 50 copies (orange), and 5 copies (gray) of Mpox-positive control DNA were tested for each primer/probe set. Back Lot-to-lot consistency of 5X PrimePath Probe qPCR Kit, ROX plus, GPR Lot-to-lot consistency of 5X PrimePath Probe qPCR Kit, ROX plus, GPR. Three separate lots of 5X PrimePath Probe qPCR Kit, ROX plus, GPR were used to amplify 100 ng, 10 ng, 1 ng, 100 pg, or 10 pg of human cDNA using a human GAPDH primer/probe set. The Cq for each amount of human cDNA using Lot 1, Lot 2, or Lot 3 are shown. Back Inhibitor tolerance of 5X PrimePath Probe qPCR Kit, ROX plus, GPR compared to competitor products Inhibitor tolerance of 5X PrimePath Probe qPCR Kit, ROX plus, GPR compared to competitor products. Multiplex assays for the detection of GAPDH, ACTB, and RNAse P from human cDNA were performed using 5X PrimePath Probe qPCR Kit, ROX plus, TaqPath qPCR Master Mix GC (ThermoFisher Scientific) or PerfeCTa qPCR ToughMix (Quantbio) both with and without inhibitors: hematin porcine (80 µM), heparin (50 mU), or urea (800 mM). To show performance changes in the presence of inhibitors, the ΔCq for each sample was calculated by determining the difference between the Cq value of inhibited samples and the no-inhibitor control. As demonstrated for each gene in the multiplex assay, 5X PrimePath Probe qPCR Kit, ROX plus, GPR had low ΔCq values across all target genes, indicating comparable performance between inhibited and uninhibited samples.
638347	5X PrimePath™ Probe qPCR Kit, no ROX, GPR	1,000 Rxns	USD $1429.00
The 5X PrimePath Probe qPCR Kit, no ROX, GPR is a real-time PCR master mix containing TaKaRa Taq HS premixed with the buffers needed for probe-based qPCR using DNA samples. The 5X concentration of the master mix allows for increased sample volumes to improve sensitivity or the ability to add additional primer/probes for the development of multiplex assays. Cat. # 638347 contains reagents sufficient to perform 1,000 x 25 µl reactions. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Dynamic range of 5X PrimePath Probe qPCR Kit, no ROX, GPR Dynamic range of 5X PrimePath Probe qPCR Kit, no ROX, GPR. Human cDNA was amplified using human GAPDH primers/probes with 5X PrimePath Probe qPCR Kit, no ROX plus, GPR on a CFX96 Real-Time PCR System. Two replicates of a sixfold dilution series of human cDNA were tested. The amplification plot for the dilution series is shown. Back Lot-to-lot consistency of 5X PrimePath Probe qPCR Kit, no ROX, GPR Lot-to-lot consistency of 5X PrimePath Probe qPCR Kit, no ROX, GPR. Three separate lots of 5X PrimePath Probe qPCR Kit, no ROX, GPR were used to amplify 100 ng, 10 ng, 1 ng, 100 pg, or 10 pg of human cDNA using a human GAPDH primer/probe set. The Cq for each amount of human cDNA using Lot 1, Lot 2, or Lot 3 of 5X PrimePath Probe qPCR Kit, no ROX, GPR are shown
638348	5X PrimePath™ Probe qPCR Kit, ROX plus, GPR	1,000 Rxns	USD $1429.00
The 5X PrimePath Probe qPCR Kit, ROX plus, GPR is a real-time PCR master mix containing TaKaRa Taq HS premixed with ROX dye and the buffers needed for probe-based qPCR using DNA samples. The 5X concentration of the master mix allows for increased sample volumes to improve sensitivity or the ability to add additional primer/probes for the development of multiplex assays. Cat. # 638348 contains reagents sufficient to perform 1,000 x 25 µl reactions. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Dynamic range of 5X Primepath Probe qPCR Kit, ROX plus, GPR Dynamic range of 5X Primepath Probe qPCR Kit, ROX plus, GPR. Human cDNA was amplified using human GAPDH primers/probes with 5X PrimePath Probe qPCR Kit, ROX plus, GPR on a QuantStudio Real-Time PCR System. Two replicates of a sixfold dilution series consisting of 100 ng (blue), 10 ng (green), 1 ng (orange), 100 pg (gray), 10 pg (red), and 1 pg (purple) of human cDNA and a no-template control (black) were tested. The amplification plot for the dilution series is shown. Back Dynamic range of 5X PrimePath Probe qPCR Kit, ROX plus, GPR using three different Mpox primer/probe sets Dynamic range of 5X PrimePath Probe qPCR Kit, ROX plus, GPR using three different Mpox primer/probe sets. Mpox-positive control DNA was amplified using (Panel A) a generic Mpox primer/probe set, (Panel B) a West African strain primer/probe set, or a (Panel C) Congo Basin strain primer/probe set with 5X PrimePath Probe qPCR Kit, ROX plus, GPR on a QuantStudio Real-Time PCR System. Two replicates of a fivefold dilution series consisting of 50,000 copies (red), 5,000 copies (blue), 500 copies (green), 50 copies (orange), and 5 copies (gray) of Mpox-positive control DNA were tested for each primer/probe set. Back Lot-to-lot consistency of 5X PrimePath Probe qPCR Kit, ROX plus, GPR Lot-to-lot consistency of 5X PrimePath Probe qPCR Kit, ROX plus, GPR. Three separate lots of 5X PrimePath Probe qPCR Kit, ROX plus, GPR were used to amplify 100 ng, 10 ng, 1 ng, 100 pg, or 10 pg of human cDNA using a human GAPDH primer/probe set. The Cq for each amount of human cDNA using Lot 1, Lot 2, or Lot 3 are shown. Back Inhibitor tolerance of 5X PrimePath Probe qPCR Kit, ROX plus, GPR compared to competitor products Inhibitor tolerance of 5X PrimePath Probe qPCR Kit, ROX plus, GPR compared to competitor products. Multiplex assays for the detection of GAPDH, ACTB, and RNAse P from human cDNA were performed using 5X PrimePath Probe qPCR Kit, ROX plus, TaqPath qPCR Master Mix GC (ThermoFisher Scientific) or PerfeCTa qPCR ToughMix (Quantbio) both with and without inhibitors: hematin porcine (80 µM), heparin (50 mU), or urea (800 mM). To show performance changes in the presence of inhibitors, the ΔCq for each sample was calculated by determining the difference between the Cq value of inhibited samples and the no-inhibitor control. As demonstrated for each gene in the multiplex assay, 5X PrimePath Probe qPCR Kit, ROX plus, GPR had low ΔCq values across all target genes, indicating comparable performance between inhibited and uninhibited samples.

Overview

Increase the limit of detection for your qPCR-based pathogen detection assays—no preamplification needed
Develop successful multiplex assays with increased reaction space for multiple primer/probe sets
Go from reaction setup to detection in less than one hour
Ensure high reproducibility and consistent performance across reactions with a general purpose reagent labeled “For Laboratory Use”

More Information

Applications

Diagnostic assay development
Pathogen detection (viruses and/or bacteria)
Multiplex real-time PCR

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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5X PrimePath Probe qPCR Kit, GPR

Notice to purchaser

Dynamic range of 5X PrimePath Probe qPCR Kit, no ROX, GPR

Lot-to-lot consistency of 5X PrimePath Probe qPCR Kit, no ROX, GPR

Notice to purchaser

Dynamic range of 5X Primepath Probe qPCR Kit, ROX plus, GPR

Dynamic range of 5X PrimePath Probe qPCR Kit, ROX plus, GPR using three different Mpox primer/probe sets

Lot-to-lot consistency of 5X PrimePath Probe qPCR Kit, ROX plus, GPR

Inhibitor tolerance of 5X PrimePath Probe qPCR Kit, ROX plus, GPR compared to competitor products

Notice to purchaser

Dynamic range of 5X PrimePath Probe qPCR Kit, no ROX, GPR

Lot-to-lot consistency of 5X PrimePath Probe qPCR Kit, no ROX, GPR

Notice to purchaser

Dynamic range of 5X Primepath Probe qPCR Kit, ROX plus, GPR

Dynamic range of 5X PrimePath Probe qPCR Kit, ROX plus, GPR using three different Mpox primer/probe sets

Lot-to-lot consistency of 5X PrimePath Probe qPCR Kit, ROX plus, GPR

Inhibitor tolerance of 5X PrimePath Probe qPCR Kit, ROX plus, GPR compared to competitor products

Overview

More Information

Applications

Additional product information