pCold TF DNA
Our pCold TF DNA vector is a fusion cold shock expression vector that expresses trigger factor (TF) chaperone as a soluble fusion tag. Trigger factor is a prokaryotic ribosome-associated chaperone protein of 48 kDa which facilitates co-translational folding of nascent polypeptides, reducing protein misfolding and production of insoluble protein. Because of its E. coli origin, TF is highly expressed in E. coli expression systems. The pCold TF DNA vector contains a cspA promoter plus additional downstream sequences including a 5' untranslated region (5' UTR), a translation enhancing element (TEE), a his-tag sequence and a multiple cloning site (MCS). A lac operator is inserted downstream of the cspA promoter to ensure strict regulation of expression. Additionally, recognition sites for HRV 3C Protease, Thrombin and Factor Xa are located between the MCS and TF/His fusion tags and function to facilitate tag removal from the expressed fusion protein. Most E. coli strains can serve as expression hosts.
GenBank Accession No: AB213654
- Highly efficient protein expression using cold shock technology
- High yield of active protein due to the trigger factor chaperone solubility-promoting fusion tag
- Contains over 70% double-stranded covalently closed circular DNA (RF I)
- Sequencing-verified cloning sites (using dideoxy sequencing method)
- Single restriction site cleavage proven by restriction enzymes NdeI, SacI, KpnI, XhoI, BamHI, EcoRI, HindIII, SalI, PstI and XbaI
Gerlined, S. et al. EMBO J. 14:4939–4948 (1995).
Qing, G. et al. Cold-shock induced high-yield protein production in Escherichia coli. Nature Biotechnol. 22:877–2004 (2004).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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