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  • ‹ Back to Single-cell RNA-seq
  • SMART-Seq mRNA Single Cell LP and SMART-Seq mRNA Single Cell
  • SMART-Seq Single Cell for scRNA-seq
  • SMART-Seq Stranded for total RNA-seq
  • SMART-Seq Stranded for MGI
Isoform analysis Customer data: combining droplet and full-length sequencing
Single cell 5 FACS tips for scRNA-seq
Single cells 5 tips for successful scRNA-seq experiments
NGS analysis software
Home › Products › Next-generation sequencing › RNA-seq › Single-cell RNA-seq › SMART-Seq mRNA Single Cell LP and SMART-Seq mRNA Single Cell

RNA-seq

  • Single-cell RNA-seq
    • SMART-Seq mRNA Single Cell LP and SMART-Seq mRNA Single Cell
    • SMART-Seq Single Cell for scRNA-seq
    • SMART-Seq Stranded for total RNA-seq
  • Ultra-low input RNA-seq
    • SMART-Seq mRNA LP (with UMIs)
    • SMART-Seq mRNA LP and SMART-Seq mRNA
    • SMART-Seq v4 for mRNA-seq
    • SMART-Seq v4 3’ DE for mRNA-seq end counting
    • SMART-Seq DE3 Demultiplexer
  • Stranded RNA-seq for mammalian samples
    • Pico-input strand-specific total RNA-seq for mammalian samples v3
    • Pico-input strand-specific total RNA-seq for mammalian samples v2
    • Low-input strand-specific total RNA-seq for mammalian samples
    • High-input strand-specific total RNA-seq for mammalian samples
  • Kits for automation
    • SMART-Seq HT for streamlined mRNA-seq
    • SMART-Seq v4 for mRNA-seq with the Fluidigm C1
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Isoform analysis Customer data: combining droplet and full-length sequencing
Single cell 5 FACS tips for scRNA-seq
Single cells 5 tips for successful scRNA-seq experiments
NGS analysis software

SMART-Seq mRNA Single Cell LP and SMART-Seq mRNA Single Cell

NOTE: SMART-Seq mRNA Single Cell LP and SMART-Seq mRNA Single Cell are equivalent replacements for the SMART-Seq Single Cell PLUS and SMART-Seq Single Cell with minor updates (see a complete list of kits with new names and the existing kits they will replace here).

The updates (listed below) do not in any way impact the protocols or functional performance of these kits.

  1. New kit sizes match those of Unique Dual Index kits (24 rxns, 96 rxns, 384 rxns)
  2. For added flexibility indexes are not included in the library prep (LP) kit; a choice of indexing primers are sold separately (see Unique Dual Index kits)

NOTE: SMART-Seq mRNA Single Cell LP and SMART-Seq mRNA Single Cell are equivalent replacements for the SMART-Seq Single Cell PLUS and SMART-Seq Single Cell with minor updates (see a complete list of kits with new names and the existing kits they will replace here).

The updates (listed below) do not in any way impact the protocols or functional performance of these kits.

  1. New kit sizes match those of Unique Dual Index kits (24 rxns, 96 rxns, 384 rxns)
  2. For added flexibility indexes are not included in the library prep (LP) kit; a choice of indexing primers are sold separately (see Unique Dual Index kits)

The SMART-Seq mRNA Single Cell Kit (SSmRNA Single Cell) and the SMART-Seq mRNA Single Cell LP Kit (SSmRNA Single Cell LP) are powered by robust chemistry that provides unparalleled sensitivity and reproducibility for single-cell and nuclei applications. These kits use oligo(dT) priming to generate high-quality, full-length cDNA directly from single cells known to have low RNA content (e.g., PBMCs, T cells, B cells, etc.). Using the SSmRNA Single Cell LP kit with Unique Dual Index kits (sold separately), up to 384 multiplexed Illumina-ready sequencing libraries can be generated. The kit offers an end-to-end solution from sample to cDNA synthesis, library preparation, and data analysis with our free Cogent NGS bioinformatics pipeline.

The SMART (Switching Mechanism at 5′ End of RNA Template) technology powering SSmRNA Single Cell provides full-length transcript information, enabling analysis of transcript isoforms, gene fusions, point mutations, etc. The cDNA kit provides high reproducibility, even gene-body coverage, and an accurate representation of GC-rich transcripts. We have further modified this powerful core technology to create a new chemistry with higher sensitivity designed specifically for single-cell applications. These enhancements result in more useful reads and a higher gene detection, allowing for a better understanding of precious samples.

The SSmRNA Single Cell LP Kit includes our patented library preparation kit that incorporates enzymatic fragmentation and stem-loop adapters to construct high-quality, Illumina-compatible libraries from the cDNA synthesized with the core kit. This two-step workflow takes place in a single tube and can be completed in about two hours with no intermediate purification steps necessary—minimizing sample mix-up, sample loss, and cross-contamination. The high library yield generated from this kit provides the flexibility to sequence on high-throughput sequencers while providing high reproducibility.

If you are interested in these benefits but are working with intact, high-quality cells (<1,000) or ultra-low total RNA inputs, we recommend our SMART-Seq mRNA LP Kit or our SMART-Seq mRNA LP (with UMIs) Kit.

If you prefer a random priming approach that will allow you to work with degraded samples and includes library preparation and indexing reagents, we recommend our SMART-Seq Stranded Kit.

 More  Less
Cat. # Product Size License Quantity Details
634786 SMART-Seq® mRNA Single Cell LP 24 Rxns USD $1092.00

License Statement

ID Number  
275 SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com.
385 This product is protected by U.S. Patents 7,803,550, 8,399,199, 8,728,737, and 9,598,727 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

The SMART-Seq mRNA Single Cell LP Kit is a complete kit designed to first generate high-quality cDNA from single cells and then high-quality Illumina sequencing-ready libraries. Indexes are added using a unique dual index kit. This kit supports up to 24 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Automation and miniaturization of SSsc on the mosquito HV.

Automation and miniaturization of SSsc on the mosquito HV.

Comparing full-volume versus eighth-volume processing on the mosquito HV with single CHO cells. Boxplots of gene counts for each preparation (medians: FV=16,592, Eighth=16,014). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data

Back

The SMART-Seq Single Cell PLUS Kit demonstrates greater discrimination between control and single-cell samples than NEBNext.

The SMART-Seq Single Cell PLUS Kit demonstrates greater discrimination between control and single-cell samples than NEBNext.

Clustering of the SSsc single cell and positive controls and significantly distinct discrimination of the negative controls provides confidence in the data generated with SSsc PLUS. By UMAP analysis, there is a clear separation between the negative controls and the positive controls/single-cell samples for SSsc PLUS. As expected, the positive controls (grey) and the single-cell samples (green) cluster together for the SSsc PLUS while the negative controls (dusty blue) cluster together near the bottom of the chart. Interestingly, the SSsc PLUS negative controls and all of the NEBNext samples cluster together: negative (red), positive (light blue), and the single cells (purple). These analyses indicate that experimental noise, even when working with such small amounts of starting material, will not affect confidence in the biological import of the results. Data is shown for SMART-Seq Single Cell PLUS (SSsc PLUS). SMART-Seq mRNA Single Cell LP is an equivalent replacement for SMART-Seq Single Cell PLUS.

Back

Automation and miniaturization of SSsc on the MANTIS liquid handler.

Automation and miniaturization of SSsc on the MANTIS liquid handler.

Comparing full-volume versus quarter-volume processing with single GM12878 cells on the MANTIS liquid handler. Boxplots of gene counts for each preparation (medians: FV=9,980, Quarter=9,603). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data.

Back

Performance comparison of SS2 versus SSsc with GM12878 cells and SS3 versus SSsc with primary T cells isolated from PBMCs.

Performance comparison of SS2 versus SSsc with GM12878 cells and SS3 versus SSsc with primary T cells isolated from PBMCs.

Boxplots representing the distribution of gene counts for TPM > 0.1. The boxes denote the interquartile range (IQR), i.e., the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Outliers are plotted as empty circles. Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SMART-Seq Single Cell.

Back

Comparable data between SMART-Seq library prep and Nextera XT kits enable existing users to easily make the switch.

Comparable data between SMART-Seq library prep and Nextera XT kits enable existing users to easily make the switch.

Comparable data between SMART-Seq library prep and Nextera XT kits enable existing users to easily make the switch. Representative XY-plots of two example libraries from the same cDNA prepared with SSsc PLUS or Nextera XT in duplicate, and the same cDNA library compared between Nextera XT and PLUS. R2 values are shown in the graph; Nextera XT R2=0.9961, PLUS R2=0.9978, Nextera XT vs PLUS R2=0.9579. Data is shown for SMART-Seq Single Cell PLUS (SSsc PLUS). SMART-Seq mRNA Single Cell LP is an equivalent replacement for SMART-Seq Single Cell PLUS.

Required Products

Cat. # Product Size Price License Quantity Details
634752 Unique Dual Index Kit (1–96) 96 Rxns USD $579.00

The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634753 Unique Dual Index Kit (97–192) 96 Rxns USD $579.00

The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634754 Unique Dual Index Kit (193–288) 96 Rxns USD $579.00

The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634755 Unique Dual Index Kit (289–384) 96 Rxns USD $579.00

The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634756 Unique Dual Index Kit (1–24) 24 Rxns USD $215.00

The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634787 SMART-Seq® mRNA Single Cell LP 96 Rxns USD $3744.00

License Statement

ID Number  
275 SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com.
385 This product is protected by U.S. Patents 7,803,550, 8,399,199, 8,728,737, and 9,598,727 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

The SMART-Seq mRNA Single Cell LP Kit is a complete kit designed to first generate high-quality cDNA from single cells and then high-quality Illumina sequencing-ready libraries. Indexes are added using a unique dual index kit. This kit supports up to 96 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Automation and miniaturization of SSsc on the mosquito HV.

Automation and miniaturization of SSsc on the mosquito HV.

Comparing full-volume versus eighth-volume processing on the mosquito HV with single CHO cells. Boxplots of gene counts for each preparation (medians: FV=16,592, Eighth=16,014). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data

Back

The SMART-Seq Single Cell PLUS Kit demonstrates greater discrimination between control and single-cell samples than NEBNext.

The SMART-Seq Single Cell PLUS Kit demonstrates greater discrimination between control and single-cell samples than NEBNext.

Clustering of the SSsc single cell and positive controls and significantly distinct discrimination of the negative controls provides confidence in the data generated with SSsc PLUS. By UMAP analysis, there is a clear separation between the negative controls and the positive controls/single-cell samples for SSsc PLUS. As expected, the positive controls (grey) and the single-cell samples (green) cluster together for the SSsc PLUS while the negative controls (dusty blue) cluster together near the bottom of the chart. Interestingly, the SSsc PLUS negative controls and all of the NEBNext samples cluster together: negative (red), positive (light blue), and the single cells (purple). These analyses indicate that experimental noise, even when working with such small amounts of starting material, will not affect confidence in the biological import of the results. Data is shown for SMART-Seq Single Cell PLUS (SSsc PLUS). SMART-Seq mRNA Single Cell LP is an equivalent replacement for SMART-Seq Single Cell PLUS.

Back

Automation and miniaturization of SSsc on the MANTIS liquid handler.

Automation and miniaturization of SSsc on the MANTIS liquid handler.

Comparing full-volume versus quarter-volume processing with single GM12878 cells on the MANTIS liquid handler. Boxplots of gene counts for each preparation (medians: FV=9,980, Quarter=9,603). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data.

Back

Performance comparison of SS2 versus SSsc with GM12878 cells and SS3 versus SSsc with primary T cells isolated from PBMCs.

Performance comparison of SS2 versus SSsc with GM12878 cells and SS3 versus SSsc with primary T cells isolated from PBMCs.

Boxplots representing the distribution of gene counts for TPM > 0.1. The boxes denote the interquartile range (IQR), i.e., the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Outliers are plotted as empty circles. Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SMART-Seq Single Cell.

Back

Comparable data between SMART-Seq library prep and Nextera XT kits enable existing users to easily make the switch.

Comparable data between SMART-Seq library prep and Nextera XT kits enable existing users to easily make the switch.

Comparable data between SMART-Seq library prep and Nextera XT kits enable existing users to easily make the switch. Representative XY-plots of two example libraries from the same cDNA prepared with SSsc PLUS or Nextera XT in duplicate, and the same cDNA library compared between Nextera XT and PLUS. R2 values are shown in the graph; Nextera XT R2=0.9961, PLUS R2=0.9978, Nextera XT vs PLUS R2=0.9579. Data is shown for SMART-Seq Single Cell PLUS (SSsc PLUS). SMART-Seq mRNA Single Cell LP is an equivalent replacement for SMART-Seq Single Cell PLUS.

Required Products

Cat. # Product Size Price License Quantity Details
634752 Unique Dual Index Kit (1–96) 96 Rxns USD $579.00

The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634753 Unique Dual Index Kit (97–192) 96 Rxns USD $579.00

The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634754 Unique Dual Index Kit (193–288) 96 Rxns USD $579.00

The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634755 Unique Dual Index Kit (289–384) 96 Rxns USD $579.00

The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634756 Unique Dual Index Kit (1–24) 24 Rxns USD $215.00

The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634788 SMART-Seq® mRNA Single Cell LP 4 x 96 Rxns Inquire for Quotation

License Statement

ID Number  
275 SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com.
385 This product is protected by U.S. Patents 7,803,550, 8,399,199, 8,728,737, and 9,598,727 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
*

The SMART-Seq mRNA Single Cell LP Kit is a complete kit designed to first generate high-quality cDNA from single cells and then high-quality Illumina sequencing-ready libraries. Indexes are added using a unique dual index kit. This kit supports up to 384 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Automation and miniaturization of SSsc on the mosquito HV.

Automation and miniaturization of SSsc on the mosquito HV.

Comparing full-volume versus eighth-volume processing on the mosquito HV with single CHO cells. Boxplots of gene counts for each preparation (medians: FV=16,592, Eighth=16,014). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data

Back

The SMART-Seq Single Cell PLUS Kit demonstrates greater discrimination between control and single-cell samples than NEBNext.

The SMART-Seq Single Cell PLUS Kit demonstrates greater discrimination between control and single-cell samples than NEBNext.

Clustering of the SSsc single cell and positive controls and significantly distinct discrimination of the negative controls provides confidence in the data generated with SSsc PLUS. By UMAP analysis, there is a clear separation between the negative controls and the positive controls/single-cell samples for SSsc PLUS. As expected, the positive controls (grey) and the single-cell samples (green) cluster together for the SSsc PLUS while the negative controls (dusty blue) cluster together near the bottom of the chart. Interestingly, the SSsc PLUS negative controls and all of the NEBNext samples cluster together: negative (red), positive (light blue), and the single cells (purple). These analyses indicate that experimental noise, even when working with such small amounts of starting material, will not affect confidence in the biological import of the results. Data is shown for SMART-Seq Single Cell PLUS (SSsc PLUS). SMART-Seq mRNA Single Cell LP is an equivalent replacement for SMART-Seq Single Cell PLUS.

Back

Automation and miniaturization of SSsc on the MANTIS liquid handler.

Automation and miniaturization of SSsc on the MANTIS liquid handler.

Comparing full-volume versus quarter-volume processing with single GM12878 cells on the MANTIS liquid handler. Boxplots of gene counts for each preparation (medians: FV=9,980, Quarter=9,603). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data.

Back

Performance comparison of SS2 versus SSsc with GM12878 cells and SS3 versus SSsc with primary T cells isolated from PBMCs.

Performance comparison of SS2 versus SSsc with GM12878 cells and SS3 versus SSsc with primary T cells isolated from PBMCs.

Boxplots representing the distribution of gene counts for TPM > 0.1. The boxes denote the interquartile range (IQR), i.e., the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Outliers are plotted as empty circles. Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SMART-Seq Single Cell.

Back

Comparable data between SMART-Seq library prep and Nextera XT kits enable existing users to easily make the switch.

Comparable data between SMART-Seq library prep and Nextera XT kits enable existing users to easily make the switch.

Comparable data between SMART-Seq library prep and Nextera XT kits enable existing users to easily make the switch. Representative XY-plots of two example libraries from the same cDNA prepared with SSsc PLUS or Nextera XT in duplicate, and the same cDNA library compared between Nextera XT and PLUS. R2 values are shown in the graph; Nextera XT R2=0.9961, PLUS R2=0.9978, Nextera XT vs PLUS R2=0.9579. Data is shown for SMART-Seq Single Cell PLUS (SSsc PLUS). SMART-Seq mRNA Single Cell LP is an equivalent replacement for SMART-Seq Single Cell PLUS.

Required Products

Cat. # Product Size Price License Quantity Details
634752 Unique Dual Index Kit (1–96) 96 Rxns USD $579.00

The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634753 Unique Dual Index Kit (97–192) 96 Rxns USD $579.00

The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634754 Unique Dual Index Kit (193–288) 96 Rxns USD $579.00

The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634755 Unique Dual Index Kit (289–384) 96 Rxns USD $579.00

The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634756 Unique Dual Index Kit (1–24) 24 Rxns USD $215.00

The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components
634789 SMART-Seq® mRNA Single Cell 24 Rxns USD $924.00

License Statement

ID Number  
275 SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com.
385 This product is protected by U.S. Patents 7,803,550, 8,399,199, 8,728,737, and 9,598,727 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

The SMART-Seq mRNA Single Cell Kit is designed to generate high-quality, full-length cDNA directly from single cells. It has been validated with 2 pg of total RNA input and with single cells known to have low RNA content (e.g., peripheral blood mononuclear cells). This kit supports up to 24 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Automation and miniaturization of SSsc on the mosquito HV.

Automation and miniaturization of SSsc on the mosquito HV.

Comparing full-volume versus eighth-volume processing on the mosquito HV with single CHO cells. Boxplots of gene counts for each preparation (medians: FV=16,592, Eighth=16,014). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data

Back

The SMART-Seq Single Cell PLUS Kit demonstrates greater discrimination between control and single-cell samples than NEBNext.

The SMART-Seq Single Cell PLUS Kit demonstrates greater discrimination between control and single-cell samples than NEBNext.

Clustering of the SSsc single cell and positive controls and significantly distinct discrimination of the negative controls provides confidence in the data generated with SSsc PLUS. By UMAP analysis, there is a clear separation between the negative controls and the positive controls/single-cell samples for SSsc PLUS. As expected, the positive controls (grey) and the single-cell samples (green) cluster together for the SSsc PLUS while the negative controls (dusty blue) cluster together near the bottom of the chart. Interestingly, the SSsc PLUS negative controls and all of the NEBNext samples cluster together: negative (red), positive (light blue), and the single cells (purple). These analyses indicate that experimental noise, even when working with such small amounts of starting material, will not affect confidence in the biological import of the results. Data is shown for SMART-Seq Single Cell PLUS (SSsc PLUS). SMART-Seq mRNA Single Cell LP is an equivalent replacement for SMART-Seq Single Cell PLUS.

Back

Automation and miniaturization of SSsc on the MANTIS liquid handler.

Automation and miniaturization of SSsc on the MANTIS liquid handler.

Comparing full-volume versus quarter-volume processing with single GM12878 cells on the MANTIS liquid handler. Boxplots of gene counts for each preparation (medians: FV=9,980, Quarter=9,603). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data.

Back

Performance comparison of SS2 versus SSsc with GM12878 cells and SS3 versus SSsc with primary T cells isolated from PBMCs.

Performance comparison of SS2 versus SSsc with GM12878 cells and SS3 versus SSsc with primary T cells isolated from PBMCs.

Boxplots representing the distribution of gene counts for TPM > 0.1. The boxes denote the interquartile range (IQR), i.e., the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Outliers are plotted as empty circles. Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SMART-Seq Single Cell.

634790 SMART-Seq® mRNA Single Cell 96 Rxns USD $3168.00

License Statement

ID Number  
275 SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com.
385 This product is protected by U.S. Patents 7,803,550, 8,399,199, 8,728,737, and 9,598,727 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

The SMART-Seq mRNA Single Cell Kit is designed to generate high-quality, full-length cDNA directly from single cells. It has been validated with 2 pg of total RNA input and with single cells known to have low RNA content (e.g., peripheral blood mononuclear cells). This kit supports up to 96 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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Automation and miniaturization of SSsc on the mosquito HV.

Automation and miniaturization of SSsc on the mosquito HV.

Comparing full-volume versus eighth-volume processing on the mosquito HV with single CHO cells. Boxplots of gene counts for each preparation (medians: FV=16,592, Eighth=16,014). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data

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The SMART-Seq Single Cell PLUS Kit demonstrates greater discrimination between control and single-cell samples than NEBNext.

The SMART-Seq Single Cell PLUS Kit demonstrates greater discrimination between control and single-cell samples than NEBNext.

Clustering of the SSsc single cell and positive controls and significantly distinct discrimination of the negative controls provides confidence in the data generated with SSsc PLUS. By UMAP analysis, there is a clear separation between the negative controls and the positive controls/single-cell samples for SSsc PLUS. As expected, the positive controls (grey) and the single-cell samples (green) cluster together for the SSsc PLUS while the negative controls (dusty blue) cluster together near the bottom of the chart. Interestingly, the SSsc PLUS negative controls and all of the NEBNext samples cluster together: negative (red), positive (light blue), and the single cells (purple). These analyses indicate that experimental noise, even when working with such small amounts of starting material, will not affect confidence in the biological import of the results. Data is shown for SMART-Seq Single Cell PLUS (SSsc PLUS). SMART-Seq mRNA Single Cell LP is an equivalent replacement for SMART-Seq Single Cell PLUS.

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Automation and miniaturization of SSsc on the MANTIS liquid handler.

Automation and miniaturization of SSsc on the MANTIS liquid handler.

Comparing full-volume versus quarter-volume processing with single GM12878 cells on the MANTIS liquid handler. Boxplots of gene counts for each preparation (medians: FV=9,980, Quarter=9,603). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data.

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Performance comparison of SS2 versus SSsc with GM12878 cells and SS3 versus SSsc with primary T cells isolated from PBMCs.

Performance comparison of SS2 versus SSsc with GM12878 cells and SS3 versus SSsc with primary T cells isolated from PBMCs.

Boxplots representing the distribution of gene counts for TPM > 0.1. The boxes denote the interquartile range (IQR), i.e., the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Outliers are plotted as empty circles. Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SMART-Seq Single Cell.

634785 SMART-Seq® mRNA Single Cell 4 x 96 Rxns Inquire for Quotation

License Statement

ID Number  
275 SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com.
385 This product is protected by U.S. Patents 7,803,550, 8,399,199, 8,728,737, and 9,598,727 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
*

The SMART-Seq mRNA Single Cell Kit is designed to generate high-quality, full-length cDNA directly from single cells. It has been validated with 2 pg of total RNA input and with single cells known to have low RNA content (e.g., peripheral blood mononuclear cells). This kit supports up to 384 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Automation and miniaturization of SSsc on the mosquito HV.

Automation and miniaturization of SSsc on the mosquito HV.

Comparing full-volume versus eighth-volume processing on the mosquito HV with single CHO cells. Boxplots of gene counts for each preparation (medians: FV=16,592, Eighth=16,014). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data

Back

The SMART-Seq Single Cell PLUS Kit demonstrates greater discrimination between control and single-cell samples than NEBNext.

The SMART-Seq Single Cell PLUS Kit demonstrates greater discrimination between control and single-cell samples than NEBNext.

Clustering of the SSsc single cell and positive controls and significantly distinct discrimination of the negative controls provides confidence in the data generated with SSsc PLUS. By UMAP analysis, there is a clear separation between the negative controls and the positive controls/single-cell samples for SSsc PLUS. As expected, the positive controls (grey) and the single-cell samples (green) cluster together for the SSsc PLUS while the negative controls (dusty blue) cluster together near the bottom of the chart. Interestingly, the SSsc PLUS negative controls and all of the NEBNext samples cluster together: negative (red), positive (light blue), and the single cells (purple). These analyses indicate that experimental noise, even when working with such small amounts of starting material, will not affect confidence in the biological import of the results. Data is shown for SMART-Seq Single Cell PLUS (SSsc PLUS). SMART-Seq mRNA Single Cell LP is an equivalent replacement for SMART-Seq Single Cell PLUS.

Back

Automation and miniaturization of SSsc on the MANTIS liquid handler.

Automation and miniaturization of SSsc on the MANTIS liquid handler.

Comparing full-volume versus quarter-volume processing with single GM12878 cells on the MANTIS liquid handler. Boxplots of gene counts for each preparation (medians: FV=9,980, Quarter=9,603). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data.

Back

Performance comparison of SS2 versus SSsc with GM12878 cells and SS3 versus SSsc with primary T cells isolated from PBMCs.

Performance comparison of SS2 versus SSsc with GM12878 cells and SS3 versus SSsc with primary T cells isolated from PBMCs.

Boxplots representing the distribution of gene counts for TPM > 0.1. The boxes denote the interquartile range (IQR), i.e., the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Outliers are plotted as empty circles. Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SMART-Seq Single Cell.

SSsc outperforms SS2 Customer data: smashing single-cell sensitivity
SSsc PLUS kit tech note View data generated with the SSsc PLUS kit
SMARTer NGS SMART-Seq Single Cell Kit technical note

Overview

  • Easy, plate-based workflow—direct input of single cells isolated by FACS or other methods
  • Single-tube workflows—complete cDNA synthesis and library preparation, in one tube each, for minimization of sample loss, sample mix-up, or other handling errors
  • Unparalleled sensitivity and reproducibility—get the best performance for single-cell RNA-seq (especially for cells with very low RNA content and for nuclei) with the highest gene detection
  • Robust, full-length chemistry—outperform all existing single-cell full-length methods, including Smart-seq2, with this validated protocol
  • Increased sequencing power—include unique dual indexes to allow for the pooling of multiple samples and confident sequencing on the NovaSeq™ system
  • High library yield—sequence on high-throughput sequencers with ease (such as the NovaSeq system) or confidently save extra library for future runs
  • Highly scalable workflow with flexible, miniaturized volumes—automate and use for high-throughput applications
    • SPT Labtech mosquito HV user guide
    • Formulatrix MANTIS Microfluidic Liquid Handler user guides
      • Full-volume
      • Quarter-volume

Data is shown for the SMART-Seq Single Cell Kit (SSsc) and SMART-Seq Single Cell PLUS (SSsc PLUS), which use the same chemistry as SMART-Seq mRNA Single Cell and SMART-Seq mRNA Single Cell LP.

SMART-Seq Single Cell Kit provides higher sensitivity than Smart-seq2

Validated kit backed by expert technical support

Figure 1. The SMART-Seq Single Cell Kit outperforms Smart-seq2. Single cells from the lymphoblastoid cell line GM12878 were processed with the SMART-Seq Single Cell Kit (SSsc; 18 cells) or the Smart-seq2 method (Smart-seq2; 20 cells) using 19 cycles of PCR. RNA-seq libraries were generated and sequences analyzed (after normalizing all samples to 1.75 million paired-end reads). Panel A. The read distribution was different between the two chemistries, with a drastically higher number of reads mapping to the mitochondrial genome (and therefore, fewer reads available for gene identification) with Smart-seq2 chemistry. Panel B. More genes were detected in the cells processed with SSsc. For additional data on reproducibility and sensitivity, view our technical note.

Even higher sensitivity for single-cell applications

We have further modified our core technology to create a chemistry with higher sensitivity designed specifically for single-cell applications. We demonstrated that the SSsc kit generates data with even better sensitivity and reproducibility than a kit using our core technology (SSv4).

Figure 2. The SMART-Seq Single Cell Kit's improved chemistry is highly suitable for single-cell applications. Panel A. 12 single cells from lymphoblastoid cell line GM22601 were processed with SSv4 or SSsc using 19 cycles of PCR. All samples were normalized to 1.25 million paired-end reads. The cDNA yield generated with SSsc was drastically higher than that generated with SSv4. Panel B. About 50 single PBMCs from one donor were processed with SSv4 or SSsc. About 60% more genes were detected in the cells processed with SSsc, regardless of the number of reads used for the analysis.

SSsc PLUS provides a complete workflow from cDNA through library preparation

The SMART-Seq Single Cell PLUS Kit is designed to synthesize high-quality, full-length cDNA directly from single cells, and then generate Illumina-ready sequencing libraries.

Comparison chart of output from SSsc PLUS kit vs Nextera XT kit

Figure 3. SSsc PLUS generates more libraries and identifies more genes than Nextera® XT. Panel A. The SMART-Seq Single Cell Kit was used to produce cDNA, in triplicate, from 10 pg of Mouse Brain Total RNA. Illumina-compatible libraries were then generated using either the SMART-Seq Library Prep Kit portion of the SSsc PLUS kit or Nextera XT. Libraries were sequenced on a NextSeq® 500. The reads were normalized to 4M paired-end reads and analyzed as described in the technical note. Panel B. Library yields obtained with the SSsc PLUS kit are higher than yields produced with Nextera XT. Panel C. The SSsc PLUS kit also identified more genes compared to Nextera XT.

More Information

Applications

  • cDNA synthesis from single cells for full-length transcriptome sequencing
  • cDNA outputs from SMART-Seq mRNA Single Cell can be used with SMART-Seq Library Prep (not sold separately; sold as part of SMART-Seq mRNA Single Cell LP) for Illumina sequencing

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Product Components List to determine kit components. Certificates of Analysis and Product Components Lists are located under the Documents tab.


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Uncover the full tale of your cells

Wondering how well you’ve truly studied your single-cell sample? Worry no more! The SMART-Seq Single Cell Kit's full-length chemistry lets you capture entire transcripts—giving you the power to identify rare genes and detect SNPs, fusions, and more—filling in the gaps that 3’ methods, like 10x, can miss.

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DNA-RNA-seq (purple)

Complete end-to-end RNA-seq workflows

Our SMART-Seq PLUS kits have it all. With just one kit, go from sample to sequencing-ready library. 

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