RiboGone - Mammalian—low-input ribosomal RNA removal kit for human, mouse, and rat samples

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634846: RiboGone - Mammalian

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RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Takara Bio) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Takara Bio), while retaining more noncoding reads.

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Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA

Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA. The high level of correlation between the RNA-Seq and MAQC data (R=0.860) shows that depletion of rRNA with RiboGone does not interfere with detection of other genes by RNA-seq.

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Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples

Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples.

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RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA

RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with any random-primed SMARTer RNA-Seq kit (including the SMARTer Stranded RNA-Seq Kit, the SMARTer Universal Low Input RNA Kit for Sequencing, and the SMARTer Universal Low Input RNA Library Prep Kit).

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RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Takara Bio) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Takara Bio), while retaining more noncoding reads.

Back

Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA

Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA. The high level of correlation between the RNA-Seq and MAQC data (R=0.860) shows that depletion of rRNA with RiboGone does not interfere with detection of other genes by RNA-seq.

Back

Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples

Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples.

Back

RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA

RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with any random-primed SMARTer RNA-Seq kit (including the SMARTer Stranded RNA-Seq Kit, the SMARTer Universal Low Input RNA Kit for Sequencing, and the SMARTer Universal Low Input RNA Library Prep Kit).

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634847: RiboGone - Mammalian