Methylation-specific PCR of bisulfite-treated DNA templates: TaKaRa EpiTaq HS (for bisulfite-treated DNA)

DNA methylation analysis often incorporates bisulfite treatment of DNA in order to determine the methylation status of specific CpG regions. EpiTaq HS (for bisulfite-treated DNA) is a hot-start (HS) DNA polymerase that is optimized for PCR amplification of bisulfite-treated template DNA. PCR amplification of bisulfite-treated DNA often poses technical challenges for conventional polymerases due to the presence of uracils in the treated DNA. However, EpiTaq HS polymerase and other kit reagents allow for the adjustment of magnesium and dNTP concentrations to achieve optimal amplification efficiency and specificity, enabling the amplification of challenging targets.

EpiTaq HS is a hot-start PCR enzyme coupled with an anti-Taq antibody. Prior to high-temperature denaturation, the anti-Taq antibody suppresses polymerase activity, thereby preventing non-specific amplification as a result of mispriming or primer-dimer formation prior to PCR. The presence of the anti-Taq antibody allows for conventional PCR setup with no additional denaturation step.

Cat. #	Product	Size	Price
R110A	TaKaRa EpiTaq™ HS (for bisulfite-treated DNA)	250 Units	USD $302.00
Takara EpiTaq HS (for bisulfite-treated DNA) is a DNA polymerase optimized for PCR amplifications using bisulfite-treated DNA containing uracil as template. PCR reactions with bisulfite-treated DNA often pose particular technical challenges for conventional enzymes. TaKaRa EpiTaq HS and the reagents contained in this kit, however, allow adjustment of magnesium and dNTP concentrations to achieve optimal amplification efficiencies and specificities, facilitating excellent amplifications even for targets that previously were impossible to amplify. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Resources Back PCR with bisulfite-treated HeLa genomic DNA as template PCR with bisulfite-treated HeLa genomic DNA as template. Back R110A: TaKaRa EpiTaq HS (for bisulfite-treated DNA)
R110B	TaKaRa EpiTaq™ HS (for bisulfite-treated DNA)	1,000 Units	USD $962.00
Takara EpiTaq HS (for bisulfite-treated DNA) is a DNA polymerase optimized for PCR amplifications using bisulfite-treated DNA containing uracil as template. PCR reactions with bisulfite-treated DNA often pose particular technical challenges for conventional enzymes. TaKaRa EpiTaq HS and the reagents contained in this kit, however, allow adjustment of magnesium and dNTP concentrations to achieve optimal amplification efficiencies and specificities, facilitating excellent amplifications even for targets that previously were impossible to amplify. Cat. # R110B contains 4 of Cat. # R110A. Please refer to Cat. # R110A for complete product documentation and resources. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back PCR with bisulfite-treated HeLa genomic DNA as template PCR with bisulfite-treated HeLa genomic DNA as template. Back R110B: TaKaRa EpiTaq HS (for bisulfite-treated DNA)

Overview

Superior amplification of bisulfite-treated DNA
Efficient amplification of a range of amplicons ranging from short (150 bp) to long (1,000 bp)
EpiTaq HS enzyme inhibits non-specific amplification and primer-dimer formation
Amplified product can be used directly for TA cloning applications

More Information

Applications

Methylation analysis of CpG Islands
Amplification of bisulfite-treated DNA, including DNA templates that contain uracils
DNA amplification of AT/GC rich templates

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Methylation-specific PCR of bisulfite-treated DNA templates: TaKaRa EpiTaq HS (for bisulfite-treated DNA)

Notice to purchaser

PCR with bisulfite-treated HeLa genomic DNA as template

R110A: TaKaRa EpiTaq HS (for bisulfite-treated DNA)