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5X One-Step PrimeScript III RT-qPCR Kit

The 5X One Step PrimeScript III RT-qPCR Kit contains a 5X master mix for one-step real-time, probe-based RT-qPCR (using the 5′ nuclease method). The master mix is a heat- and inhibitor-resistant reverse transcriptase, PrimeScript III RTase, in combination with an efficient, specific hot-start PCR enzyme, TaKaRa Taq HS, in an optimized reaction buffer. A quick and simple protocol allows the reverse transcription and qPCR reactions to be performed in the same tube, reducing the risk of contamination.

The 5X One Step PrimeScript III RT-qPCR Kit contains a 5X master mix for one-step real-time, probe-based RT-qPCR (using the 5′ nuclease method). The heat- and inhibitor-resistant reverse transcriptase, PrimeScript III RTase, in combination with an efficient, specific hot-start PCR enzyme, TaKaRa Taq HS, in an optimized reaction buffer. A quick and simple protocol allows the reverse transcription and qPCR reactions to be performed in the same tube, reducing the risk of contamination.

5X concentration improves the limit of detection (LoD) of the one-step RT-qPCR assay without a preamplification step. This creates the opportunity to save time and money. PrimeScript III RTase displays increased heat tolerance (up to 55°C) while maintaining the specificity and robustness of PrimeScript RT. This allows cDNA synthesis from RNA with a more complex secondary structure. It is also highly resistant to a wide variety of inhibitory substances such as heparin (blood) and humic acid (soil), allowing stable one-step real-time RT-qPCR to be performed on a wide variety of samples.

While One Step PrimeScript III RT-qPCR Mix often works well with crude RNA extracts, using a highly pure RNA sample is recommended if the target sequence is not detectable or if more accurate, stable analysis results will be needed. It can be used for various applications such as gene expression and RNA virus detection, and it is well-suited for multiplex real-time PCR.

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Cat. # Product Size Price License Quantity Details
XU0002 5X One Step PrimeScript™ III RT-qPCR Kit, no ROX, GPR 200 Rxns USD $462.00

5X One Step PrimeScript III RT-qPCR Kit contains a more concentrated version of the premix in our 2X One Step PrimeScript III RT-qPCR Mix (RR600). For example, only 5 μl of the 5X premix is needed for a 25 μl reaction versus 12.5 μl of 2X premix. This opens possibilities for using a more dilute template or multiple primers and probes.

As with the 2X premix, the 5X premix does not freeze at its storage temperature of -20℃, so a reaction can be started simply by adding the template sample, primer, and a probe for detecting the desired target. The RT enzyme displays tolerance to elevated temperatures (up to 55℃). At higher temperatures, RNA secondary structure is more relaxed and less likely to impede extension. So, the elevated temperature tolerance enables cDNA synthesis from RNA with a more complex secondary structure.

Cat.# XU0002 contains reagents sufficient to perform 200 x 25-μl reactions.

Notice to purchaser

This product is a general purpose reagent intended For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated. This product is not intended for a specific application or made for any particular clinical use. The performance characteristics of this product have not been fully established. It is the user’s responsibility to validate the performance of the product, and any component thereof, for any particular use. Resale or transfer of this product, any component thereof, or any substance produced through use of this product, or any component thereof, to any third party is expressly forbidden. To obtain additional rights, please contact your local office or go to our website for additional information.

 

Documents Components Image Data
638337 5X One Step PrimeScript™ III RT-qPCR Kit, no ROX, GPR 1,000 Rxns USD $2295.00

5X One Step PrimeScript III RT-qPCR Kit contains a more concentrated version of the premix in our 2X One Step PrimeScript III RT-qPCR Mix (RR600). For example, only 5 μl of the 5X premix is needed for a 25 μl reaction versus 12.5 μl of 2X premix. This opens possibilities for using a more dilute template or multiple primers and probes.

As with the 2X premix, the 5X premix does not freeze at its storage temperature of -20℃, so a reaction can be started simply by adding the template sample, primer, and a probe for detecting the desired target. The RT enzyme displays tolerance to elevated temperatures (up to 55℃). At higher temperatures, RNA secondary structure is more relaxed and less likely to impede extension. So, the elevated temperature tolerance enables cDNA synthesis from RNA with a more complex secondary structure.

Cat. # 638337 contains five of Cat. # XU0002, sufficient to perform 1,000 x 25-μl reactions. Please refer to Cat. # XU0002 for complete product documentation and resources.

Notice to purchaser

This product is a general purpose reagent intended For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated. This product is not intended for a specific application or made for any particular clinical use. The performance characteristics of this product have not been fully established. It is the user’s responsibility to validate the performance of the product, and any component thereof, for any particular use. Resale or transfer of this product, any component thereof, or any substance produced through use of this product, or any component thereof, to any third party is expressly forbidden. To obtain additional rights, please contact your local office or go to our website for additional information.

 

Documents Components Image Data
XU0001 5X One Step PrimeScript™ III RT-qPCR Kit, ROX plus, GPR 200 Rxns USD $462.00

5X One Step PrimeScript III RT-qPCR Kit contains a more concentrated version of the premix in our 2X One Step PrimeScript III RT-qPCR Mix (RR600). For example, only 5 μl of the 5X premix is needed for a 25 μl reaction versus 12.5 μl of 2X premix. This opens possibilities for using a more dilute template or multiple primers and probes.

As with the 2X premix, the 5X premix does not freeze at its storage temperature of -20℃, so a reaction can be started simply by adding the template sample, primer, and a probe for detecting the desired target. The RT enzyme displays tolerance to elevated temperatures (up to 55℃). At higher temperatures, RNA secondary structure is more relaxed and less likely to impede extension. So, the elevated temperature tolerance enables cDNA synthesis from RNA with a more complex secondary structure.

Cat.# XU0001 and 638336 contain ROX Reference Dye, optimized to be compatible with various qPCR cyclers, in the master mix.

Cat.# XU0001 contains reagents sufficient to perform 200 x 25-μl reactions.

Notice to purchaser

This product is a general purpose reagent intended For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated. This product is not intended for a specific application or made for any particular clinical use. The performance characteristics of this product have not been fully established. It is the user’s responsibility to validate the performance of the product, and any component thereof, for any particular use. Resale or transfer of this product, any component thereof, or any substance produced through use of this product, or any component thereof, to any third party is expressly forbidden. To obtain additional rights, please contact your local office or go to our website for additional information.

 

Documents Components Image Data
638336 5X One Step PrimeScript™ III RT-qPCR Kit, ROX plus, GPR 1,000 Rxns USD $2295.00

5X One Step PrimeScript III RT-qPCR Kit contains a more concentrated version of the premix in our 2X One Step PrimeScript III RT-qPCR Mix (RR600). For example, only 5 μl of the 5X premix is needed for a 25 μl reaction versus 12.5 μl of 2X premix. This opens possibilities for using a more dilute template or multiple primers and probes.

As with the 2X premix, the 5X premix does not freeze at its storage temperature of -20℃, so a reaction can be started simply by adding the template sample, primer, and a probe for detecting the desired target. The RT enzyme displays tolerance to elevated temperatures (up to 55℃). At higher temperatures, RNA secondary structure is more relaxed and less likely to impede extension. So, the elevated temperature tolerance enables cDNA synthesis from RNA with a more complex secondary structure.

Cat. # XU0001 and 638336 contain ROX Reference Dye, optimized to be compatible with various qPCR cyclers, in the master mix.

Cat. # 638336 contains five of Cat. # XU0001, sufficient to perform 1,000 x 25-μl reactions. Please refer to Cat. # XU0001 for complete product documentation and resources.

Notice to purchaser

This product is a general purpose reagent intended For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated. This product is not intended for a specific application or made for any particular clinical use. The performance characteristics of this product have not been fully established. It is the user’s responsibility to validate the performance of the product, and any component thereof, for any particular use. Resale or transfer of this product, any component thereof, or any substance produced through use of this product, or any component thereof, to any third party is expressly forbidden. To obtain additional rights, please contact your local office or go to our website for additional information.

 

Documents Components Image Data

Overview

  • Fast, easy one-step RT-qPCR in a single tube using probe-based detection without the need for any preamplification
  • Efficient, specific cDNA synthesis and amplification, even from complex RNA templates
  • Suitable for a wide variety of samples from different sources, including crude RNA extracts
  • Compatible with automation systems such as the SmartChip Real-Time PCR System 

More Information

Applications

  • Automated gene expression analysis
  • Detection of RNA viruses
  • Multiplex real-time PCR

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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