Our research-grade retrovirus production service lets you choose from multiple expression plasmid vectors depending on the expression type you desire. Stable-cell-line and iPS-cell generation are also available.
| Expression plasmid vector options | |
| Expression type |
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| Control vector |
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| Virus envelope |
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Retroviral vector production
- Plasmid construction and preparation
- Cloning of your gene of interest (GOI) into the expression plasmid vector of your choice
- Large-scale preparation of plasmid DNA for retroviral vector production
- Producer cell production (optional)
- Recommended when performing retroviral vector transduction to human PBMCs.
- Retroviral vector production
- Transfection of 293T cells with plasmid DNA (e.g., pDON-5-GOI and packaging plasmid DNA)
- Transient expression of retroviral vector and virus collection
- Viral titration by real-time PCR
- Large-scale retroviral vector production
- Production of retroviral vector at the requested scale
- Stable-cell-line generation (optional)
- Transfection of target cell line using the retroviral vector
- Antibiotic selection of cells expressing GOI
- Confirmation of GOI expression by real-time PCR
