We offer virus production services for adeno-associated viral (AAV), lentiviral, and retroviral vectors. Other viral vectors are also available upon technical transfer.

Watch the video below to learn all about our small- and large-scale viral vector manufacturing workflows.

Our one-stop GMP manufacturing facility can support a wide range of manufacturing needs, from small-scale prototype manufacturing in the early stages of development to large-scale manufacturing up to 2,000 L. We recommend that you start with small-scale manufacturing in order to assess our manufacturing capability and to make any needed adjustments. We can accommodate special requests regarding culture vessels or containers (such as vials or bags) for the final product.

Overview of the viral vector manufacturing workflow.

AAV vectors can transduce into proliferating cells and impart long-term expression. Furthermore, AAV vectors have very low immunogenicity. For these reasons, interest in the use of AAV vectors in gene therapy is rapidly growing. As each serotype shows different characteristics and tissue specificity, AAV vectors are expected to transduce target genes efficiently into heart, liver, muscle, retina, and central nervous system tissues.

• Project initiation: we require plasmid DNA (transfer plasmid, packaging plasmids) and a producer cell line.
  NEW! Takara Bio now provides gene therapy vector CereAAV™, which is an AAV2-based serotype with enhanced brain tropism through genetic engineering and proprietary screening technology. Studies show CereAAV has a gene transfer efficiency 50 times higher in mice and 20 times higher in primate models (e.g. marmosets) than AAV9.
• Deliverables: we will deliver AAV vector, manufacturing report, and certificate of analysis.

Lentiviral vectors can transduce both replicating and nonreplicating cells. They are generally produced at titers that are higher than retrovirus, and they integrate into the host genome, allowing stable, long-term expression of the transgene. Furthermore, lentiviral vectors elicit a relatively low immune response, making them a popular choice for gene therapy applications.

• Project initiation: both plasmid DNA (transfer plasmid, packaging plasmids) and a producer cell line (293T MCB is available from Takara Bio upon request) are required for initiating the project. Takara Bio can provide LVpro Packaging Mix with various pLVpro series vectors. Please contact us if you require a lentivirus plasmid vector suitable for clinical use. 
• Deliverables: we will deliver a lentiviral vector, a manufacturing report, and a certificate of analysis.

Retroviral vectors transduce a wide range of hard-to-infect dividing cells and integrate into the host genome, which makes them ideal for long-term, stable expression of your transgene.

• Project initiation: we require plasmid DNA and a packaging cell line (PG13 packaging cell line is available from Takara Bio upon request)
• Deliverables: we will deliver a Master Cell Bank (MCB), a retroviral vector, a manufacturing report, and a certificate of analysis

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