1. MCB preparation
In addition to developing and preparing the MCB of the recombinant E.coli carrying your plasmid of interest, we produce working cell banks (WCB) and store the MCB/WCBs.
2. Pilot manufacturing
Before actual manufacturing, we optimize culturing and purification conditions, and confirm plasmid yield on a small scale.
3. Final plasmid DNA production
Plasmid DNA is manufactured from the MCB in 25 L, 2 x 25 L, or 200 L single-use systems, or in a 2,000 L stainless-steel bioreactor in accordance with GMP processes, depending on the desired scale. Our facility is also able to perform automatic, aseptic filling, vialing, and labeling.