iMatrix-511 (recombinant laminin-511): pluripotent stem cell culture matrix

Propagation of embryonic stem (ES) cells or induced pluripotent stem (iPS) cells requires a culture substrate that retains pluripotency, a stable karyotype, and expression of pluripotency markers. iMatrix-511 is a recombinant laminin-511 E8 fragment product that can be used as an ES or iPS cell-culture substrate. These recombinant laminin-511 E8 fragments permit maintenance of human ES and iPS cells in xeno-free culture conditions and provide greater adhesive properties than either full-length laminin, vitronectin, or undefined basement membrane reagents such as Matrigel. The iMatrix-511 cell-culture reagent allows stem cell culture in the absence of mouse feeder cells.

Recombinant laminin-511 E8 fragments are truncated, functional forms of laminin composed of the C-terminal regions of the alpha, beta, and gamma chains. Laminin-511 E8 (150 kDa) retains the full capability for binding to alpha 6 beta 1 integrin. Based on research performed at Kyoto University and Osaka University, recombinant laminin-511 E8 fragments are available as iMatrix-511. This product is available through an agreement between Takara Bio Inc. and Nippi, Inc.

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Cat. #	Product	Size
T304	iMatrix-511	6 x 175 ug	USD $1737.00
Laminin-511 is well known to bind to integrin α6ß1, which is located on the cell surface. iMatrix-511 is a recombinant fragment of laminin-511 which acts as a substrate for stem cell culture media for ESCs and iPSCs. iMatrix-511 is chemically defined, animal-free, and xeno-free. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like
T303	iMatrix-511	2 x 175 ug	USD $727.00
Laminin-511 is well known to bind to integrin α6ß1, which is located on the cell surface. iMatrix-511 is a recombinant fragment of laminin-511 which acts as a substrate for stem cell culture media for ESCs and iPSCs. iMatrix-511 is chemically defined, animal-free, and xeno-free. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Resources

Overview

Chemically defined stem cell culture substrate for animal-free, xeno-free, feeder-free ES or iPS cell culture
Provides greater adhesion of human ES and iPS cells than Matrigel or full-length laminin
Laminin-511 E8 fragments sustain long-term self-renewal of human ES and iPS cells in defined xeno-free media with dissociated cell passaging
Promotes high expression of pluripotency markers, normal karyotype, and retained ability to differentiate into all three germ layers

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Applications

Stem cell culture under chemically defined, animal-free, xeno-free conditions
Expansion culture of ES and iPS cells

Components

iMatrix is available in both lyophilized and liquid formats:

Cat. # T300: 350 µg recombinant laminin-511 E8 fragments (lyophilized)
Cat. # T303: 350 µg recombinant laminin-511 E8 fragments (liquid, 0.5 mg/ml)
Cat. # T304: 1,050 µg recombinant laminin-511 E8 fragments (liquid, 0.5 mg/ml)

Product citations

Ido, H. et al. The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin ?? chains in integrin binding by laminins. J. Biol. Chem. 282, 11144-11154 (2007).

Miyazaki, T. et al. Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells. Nat. Commun. 3, 1236 (2012).

Taniguchi, Y. et al. The C-terminal region of laminin ?? chains modulates the integrin binding affinities of laminins. J. Biol. Chem. 284, 7820-7831 (2009).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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