One-step PrimeScript RT-PCR kit for real-time RT-PCR

Real-time RT-PCR is made easy with the One Step PrimeScript RT-PCR Kit (Perfect Real Time), a one-step real-time RT-PCR kit designed for real-time RT-PCR (qPCR) via probe-based qPCR assays such as 5' nuclease assays¹. In this 5' nuclease assay real-time PCR (qPCR) kit, one-step real-time RT-PCR can be performed in a single tube, simplifying amplification and minimizing the risk of contamination. Amplified products are monitored in real time, so there is no need to verify them through electrophoresis following PCR. The sensitivity provided by the enzymes in this kit enables detection of very small amounts of RNA, including RNA derived from viral, rare, or precious samples.

This kit uses PrimeScript RTase, a modified, recombinant MMLV (Moloney Murine Leukemia Virus) reverse transcriptase that is verified to have low levels of RNase H and which exhibits an excellent extension rate and quick, full-length cDNA synthesis, as well as Takara Ex Taq HS, a high efficiency hot start PCR enzyme optimized for this one-step real-time RT-PCR kit.

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Cat. #	Product	Size	Price
RR064A	One Step PrimeScript™ RT-PCR Kit (Perfect Real Time)	100 Rxns	USD $424.00
The One Step PrimeScript RT-PCR Kit (Perfect Real Time) is designed for one step, real-time reverse transcription PCR (RT-PCR) using probe detection. With this kit, all RT-PCR steps can be performed in a single tube. Therefore, it is not necessary to add additional reagents during the reaction, minimizing the risk of contamination and simplifying the workflow. Amplified products are monitored in real time without need for gel electrophoresis after PCR. This kit is suitable for detection of small amounts of RNA. This kit uses PrimeScript Reverse Transcriptase, which allows efficient and rapid cDNA synthesis, and Takara Ex Taq HS, which enables highly efficient hot start PCR. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Resources Back RR064A: One Step PrimeScript RT-PCR Kit (Perfect Real Time)
RR064B	One Step PrimeScript™ RT-PCR Kit (Perfect Real Time)	500 Rxns	USD $1711.00
The One Step PrimeScript RT-PCR Kit (Perfect Real Time) is designed for one step, real-time reverse transcription PCR (RT-PCR) using probe detection. With this kit, all RT-PCR steps can be performed in a single tube. Therefore, it is not necessary to add additional reagents during the reaction, minimizing the risk of contamination and simplifying the workflow. Amplified products are monitored in real time without need for gel electrophoresis after PCR. This kit is suitable for detection of small amounts of RNA. This kit uses PrimeScript Reverse Transcriptase, which allows efficient and rapid cDNA synthesis, and Takara Ex Taq HS, which enables highly efficient hot start PCR. Cat. # RR064B contains 5 of Cat. # RR064A. Please refer to Cat. # RR064A for complete product documentation and resources. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back RR064B: One Step PrimeScript RT-PCR Kit (Perfect Real Time)

Overview

Enables accurate and rapid analysis of viral RNA or RNA derived from rare or precious sources.
Contains Takara Ex Taq HS, a high-efficiency, hot-start PCR enzyme, for PCR.
Buffer system is real-time PCR optimized, increasing amplification efficiency and detection sensitivity.

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Applications

One-step real-time RT-PCR
Probe-based qPCR assays such as 5' nuclease assays and real-time PCR (qPCR) assays

Choosing a PrimeScript kit for real time RT-PCR
If you want to....	Use this....
Make cDNA using a master mix that includes RTase and primers	PrimeScript RT Master Mix (Perfect Real Time) (Cat. # RR036A and RR036B)
Make cDNA using a kit that provides RTase and primers as separate components	PrimeScript RT Reagent Kit (Perfect Real Time) (Cat. # RR037A and RR037B)
Avoid genomic DNA contamination during cDNA synthesis and RT-qPCR	PrimeScript RT Reagent Kit with gDNA Eraser (Perfect Real Time) (Cat. # RR047A and RR047B)
Perform 1-step real-time RT-PCR with TB Green	One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) (Cat. # RR086A and RR086B)
Prepare cDNA for RT-qPCR directly from fewer than 1,000 cells	CellAmp Whole Transcriptome Amplification Kit (Real Time), Ver. 2 (Cat. # 3734)

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Notes

1. Use One Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) for real-time one-step RT-PCR using TB Green for detection.
2. Thermal Cycler Dice Real Time System is not sold worldwide. Please check for availability in your region.

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