One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time)
The One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) is a quantitative real-time PCR kit designed for intercalating dye-enabled product detection. Using this TB Green Real-Time PCR (qPCR) kit, RT-PCR can be performed in a single tube, minimizing pipetting steps and contamination risk. Reaction products are monitored in real time, eliminating the need for electrophoresis verification following PCR. The sensitivity of the enzyme offered in this intercalator real-time PCR kit enables detection of very small amounts of RNA, including RNA from viral, rare, and precious samples.
The One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) is a quantitative real-time PCR kit designed for intercalating dye-enabled product detection. Using this TB Green Real-Time PCR (qPCR) kit, RT-PCR can be performed in a single tube, minimizing pipetting steps and contamination risk. Reaction products are monitored in real time, eliminating the need for electrophoresis verification following PCR. The sensitivity of the enzyme offered in this intercalator real-time PCR kit enables detection of very small amounts of RNA, including RNA from viral, rare, and precious samples.
The One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) takes advantage of PrimeScript RTase, an MMLV-based RNase H Minus reverse transcriptase that offers robust extension rates and synthesizes full-length cDNA quickly and easily. The kit also utilizes the Takara Ex Taq HS high-efficiency hot-start PCR enzyme. Both kit enzymes are optimized for one-step RT-PCR via an optimized buffer. Combining Takara Bio RT-PCR technology with the enzymes listed below allows this one-step RT-PCR kit to generate high product yields. The premix formulation of the One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) also offers user convenience via a simplified protocol.
Overview
- Quantitative real-time PCR enables rapid and accurate analysis of RNA from viral, rare, or precious samples.
- Takara Ex Taq HS is a high-efficiency hot-start PCR enzyme that can be used in RT-PCR.
- The One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) buffer system is optimized for quantitative real-time PCR, allowing efficient amplification and highly sensitive detection.
- One-Step TB Green RT-PCR Buffer IV is a 2X TB Green premix; the premix formulation simplifies reaction mixture preparation.
More Information
Applications
- Quantitative real-time PCR
- Intercalating dye-based real-time PCR (qPCR)
- Detection and analysis of small RNA amounts
Product citations
Arita, M., Wakita, T. & Shimizu, H. Characterization of pharmacologically active compounds that inhibit poliovirus and enterovirus 71 infectivity. J. Gen. Virol. 89, 2518-30 (2008).
Murata, T. et al. Involvement of Jun dimerization protein 2 (JDP2) in the maintenance of Epstein-Barr virus latency. J. Biol. Chem. 286, 22007-16 (2011).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Powered by BiozChoosing a PrimeScript kit for real-time RT-PCR
| If you want to... | Use this |
| Make cDNA using a master mix that includes RTase and primers | PrimeScript RT Master Mix (Perfect Real Time) (Cat. # RR036A and RR036B) |
| Make cDNA using a kit that provides RTase and primers as separate components | PrimeScript RT Reagent Kit (Perfect Real Time) (Cat. # RR037A and RR037B) |
| Avoid genomic DNA contamination during cDNA synthesis and RT-qPCR | PrimeScript RT Reagent Kit with gDNA Eraser (Perfect Real Time) (Cat. # RR047A and RR047B) |
| Perform 1-step real-time RT-PCR with TB Green | One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) (Cat. # RR086A and RR086B) |
| Prepare cDNA for RT-qPCR directly from fewer than 1,000 cells | CellAmp Whole Transcriptome Amplification Kit (Real Time), Ver. 2 (Cat. # 3734) |
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