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TB Green Premix Ex Taq (Tli RNase H Plus)

TB Green Premix Ex Taq (Tli RNase H Plus), an intercalating dye-based qPCR master mix for real-time RT-PCR (qPCR), is recommended for most real-time PCR applications including gene expression analyses, genotyping, and high-throughput studies. TB Green Premix Ex Taq (Tli RNase H Plus) includes TB Green, a qPCR reagent for intercalator-based RT-PCR (qPCR). In addition to being ideal for most standard real-time PCR reactions, this kit is recommended when a higher yield of PCR product is needed or when amplifying longer products (up to 570 bp). This TB Green qPCR premix is supplied at a 2X concentration and allows easy reaction assembly; by simply adding primers, template, and sterile distilled water, intercalator-based qPCR can be performed.

TB Green Premix Ex Taq (Tli RNase H Plus), an intercalating dye-based qPCR master mix for real-time RT-PCR (qPCR), is recommended for most real-time PCR applications including gene expression analyses, genotyping, and high-throughput studies. TB Green Premix Ex Taq (Tli RNase H Plus) includes TB Green, a qPCR reagent for intercalator-based RT-PCR (qPCR). In addition to being ideal for most standard real-time PCR reactions, this kit is recommended when a higher yield of PCR product is needed or when amplifying longer products (up to 570 bp). This TB Green qPCR premix is supplied at a 2X concentration and allows easy reaction assembly; by simply adding primers, template, and sterile distilled water, intercalator-based qPCR can be performed.

TB Green Premix Ex Taq (Tli RNase H Plus) contains Takara Ex Taq HS, a hot-start PCR enzyme that includes an anti-Taq antibody. The Takara Ex Taq HS enzyme, in combination with its qPCR optimized buffer, allows for high amplification efficiency and detection sensitivity in qPCR. The 2X TB Green qPCR premix also contains Tli RNase H, a heat-resistant RNase H that minimizes qPCR inhibition as a consequence of residual mRNA in the input template cDNA. This product enables accurate detection of targets even during high-speed reactions, making it possible to conduct fast RT-PCR (qPCR) analyses and achieve excellent reproducibility and reliability.

ROX Reference Dyes are supplied as separate components with Cat. # RR420A, RR420B, RR420L, and RR420W. These reference dyes serve as convenient internal reference standards when normalizing non-PCR generated fluorescence fluctuations; such fluctuations often occur between different wells or over time in different instruments.

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Cat. # Product Size Price License Quantity Details
RR42LR TB Green® Premix Ex Taq™ (Tli RNase H Plus), 1 x 5 mL, ROX Plus 200 Rxns USD $442.00

TB Green Premix Ex Taq (Tli RNase H Plus) provides reduced PCR inhibition caused by the presence of double stranded RNA/cDNA hybrids remaining after cDNA synthesis. This inhibition is common when using low or RNase H minus RTs, and with GC rich templates and/or genes with poor expression. The presence of dsRNA/cDNA hybrids is a frequent cause of poor qPCR amplification and/or reaction failure. TB Green Premix Ex Taq (Tli RNase H Plus) prevents this potential problem at no additional cost and without requiring a separate RNase H digestion step prior to qPCR. Cat.#RR42LR and RR42WR are larger package sizes [5 ml and 25 ml (5 x 5 ml), respectively], that contain ROX Reference Dye in the premix.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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RR420A TB Green® Premix Ex Taq™ (Tli RNase H Plus) 200 Rxns USD $439.00

TB Green Premix Ex Taq (Tli RNase H Plus) provides reduced PCR inhibition caused by the presence of double-stranded RNA/cDNA hybrids remaining after cDNA synthesis. This inhibition is common when using low or RNase H minus RTs, and with GC-rich templates and/or genes with poor expression. The presence of dsRNA/cDNA hybrids is a frequent cause of poor qPCR amplification and/or reaction failure. TB Green Premix Ex Taq (Tli RNase H Plus) prevents this potential problem at no additional cost and without requiring a separate RNase H digestion step prior to qPCR.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data
RR420B TB Green® Premix Ex Taq™ (Tli RNase H Plus) 400 Rxns USD $795.00

TB Green Premix Ex Taq (Tli RNase H Plus) provides reduced PCR inhibition caused by the presence of double-stranded RNA/cDNA hybrids remaining after cDNA synthesis. This inhibition is common when using low or RNase H minus RTs, and with GC-rich templates and/or genes with poor expression. The presence of dsRNA/cDNA hybrids is a frequent cause of poor qPCR amplification and/or reaction failure. TB Green Premix Ex Taq (Tli RNase H Plus) prevents this potential problem at no additional cost and without requiring a separate RNase H digestion step prior to qPCR. Cat. # RR420B contains 2 of Cat. # RR420A. Please refer to Cat. # RR420A for complete product documentation and resources.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data
RR420L TB Green® Premix Ex Taq™ (Tli RNase H Plus), 1 x 5 mL 200 Rxns USD $442.00

TB Green Premix Ex Taq (Tli RNase H Plus) provides reduced PCR inhibition caused by the presence of double stranded RNA/cDNA hybrids remaining after cDNA synthesis. This inhibition is common when using low or RNase H minus RTs, and with GC rich templates and/or genes with poor expression. The presence of dsRNA/cDNA hybrids is a frequent cause of poor qPCR amplification and/or reaction failure. TB Green Premix Ex Taq (Tli RNase H Plus) prevents this potential problem at no additional cost and without requiring a separate RNase H digestion step prior to qPCR. Cat.# RR420L and RR420W are larger package sizes [5 ml and 25 ml (5 x 5 ml), respectively]. Each provide convenient larger packaging and are supplied with a single, separate vial of ROX Reference Dye.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data
RR420W TB Green® Premix Ex Taq™ (Tli RNase H Plus), 5 x 5 mL 1,000 Rxns USD $1627.00

TB Green Premix Ex Taq (Tli RNase H Plus) provides reduced PCR inhibition caused by the presence of double-stranded RNA/cDNA hybrids remaining after cDNA synthesis. This inhibition is common when using low or RNase H-minus RTs, and with GC-rich templates and/or genes with poor expression. The presence of dsRNA/cDNA hybrids is a frequent cause of poor qPCR amplification and/or reaction failure. TB Green Premix Ex Taq (Tli RNase H Plus) prevents this potential problem at no additional cost and without requiring a separate RNase H digestion step prior to qPCR. Cat. # RR420W contains five of Cat. # RR420L. Please refer to Cat. # RR420L for complete product documentation and resources.




Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data
RR42WR TB Green® Premix Ex Taq™ (Tli RNase H Plus), 5 x 5 mL, ROX Plus 1,000 Rxns USD $1627.00

TB Green Premix Ex Taq (Tli RNase H Plus) provides reduced PCR inhibition caused by the presence of double stranded RNA/cDNA hybrids remaining after cDNA synthesis. This inhibition is common when using low or RNase H minus RTs, and with GC rich templates and/or genes with poor expression. The presence of dsRNA/cDNA hybrids is a frequent cause of poor qPCR amplification and/or reaction failure. TB Green Premix Ex Taq (Tli RNase H Plus) prevents this potential problem at no additional cost and without requiring a separate RNase H digestion step prior to qPCR. Cat. # RR42WR contains 5 of Cat. # RR42LR. Please refer to Cat. # RR42LR for complete product documentation and resources.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

Overview

  • Lower Cq values as a result of fast extension rates
  • Use with broad range of amplicon sizes, even over 500 bp
  • Convenient 2X master mix is excellent for high-speed qPCR
  • High sensitivity: detects fewer than 100 copies
  • Hot-start PCR enzyme enables high efficiency and sensitivity in real-time PCR (qPCR)
  • Compatible with all commonly used real-time PCR (qPCR) instruments
  • Minimizes qPCR inhibition due to residual mRNA in input cDNA
  • Includes TB Green for intercalator-based qPCR

More Information

Applications

  • TB Green qPCR using TB Green and ROX Reference Dye
  • Gene expression analysis
  • Genotyping by real-time PCR
  • High-throughput real-time PCR
  • Amplification of long products

Product citations

Kim, et al. Characterization of naturally Epstein-Barr virus-infected gastric carcinoma cell line YCCEL1. J. Gen. Virol. 94, 497–506 (2013).

Kotani, et al. Systemic Circulation and Bone Recruitment of Osteoclast Precursors Tracked by Using Fluorescent Imaging Techniques. J. Immunol. 190, 605–612 (2013).

Liu, et al. Metabolism and Pharmacokinetics of Mangiferin in Conventional Rats, Pseduo-Germ-Free Rats, and Streptozotocin-Induced Diabetic Rats. Drug Metab. Dispos. 40, 2109–2118 (2012).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab within the product table above.

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