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Whole-genome amplification

Whole-genome amplification kits

The PicoPLEX WGA family of products is comprised of three versions. All of the systems below can amplify DNA directly from cell lysates from 1–10 cells and can also be used with isolated gDNA inputs ranging from less than 6 pg up to 50 pg. They are intended for use with array CGH, qPCR, and NGS applications. For reproductive health applications, we recommend using the original PicoPLEX WGA Kit (Cat. # R30050).

The PicoPLEX WGA family of products is comprised of three versions. All of the systems below can amplify DNA directly from cell lysates from 1–10 cells and can also be used with isolated gDNA inputs ranging from less than 6 pg up to 50 pg. They are intended for use with array CGH, qPCR, and NGS applications. For reproductive health applications, we recommend using the original PicoPLEX WGA Kit (Cat. # R30050).

The specific applications for each version are described below:

PicoPLEX WGA Kit (Cat. # R30050):

The PicoPLEX WGA Kit is currently used in a broad range of assays for measuring copy number variation (CNV). This is the kit of choice for assays that need the most accurate and reproducible measurement of CNV. This is the recommended product for PGT-A and other reproductive health applications.

PicoPLEX Single Cell WGA Kit (Cat. # R300671, R300672, R300673):

The PicoPLEX Single Cell WGA kit is an updated version of PicoPLEX WGA is also suited for CNV detection while producing higher yields and longer amplicons. This kit is best suited for applications where high yield is most important.

PicoPLEX Single Cell WGA Kit v3 (Cat. # R300718, R300722, R300723):

This is the latest version of PicoPLEX WGA that includes a high-fidelity amplification enzyme. Suited for applications where base-level resolution, such as detection of mutations (single nucleotide variants, SNVs), is desired. The kit also provides excellent performance in CNV detection.

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Cat. # Product Size License Quantity Details
R300671 PicoPLEX® Single Cell WGA Kit 24 Rxns USD $594.00

The PicoPLEX Single Cell WGA Kit uses a single-tube protocol developed specifically for reproducible amplification of single-copy genomic DNA (gDNA) starting with 1–10 cells or equivalent picogram quantities of isolated gDNA. Cell lysis and whole-genome preamplification are followed by amplification, which results in libraries with very low background and typical yields of 8–12 µg of product in under 3 hours. This system is best suited for applications in which high yield is most important. This product contains reagents for 24 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data
R300672 PicoPLEX® Single Cell WGA Kit 96 Rxns USD $2058.00

The PicoPLEX Single Cell WGA Kit uses a single-tube protocol developed specifically for reproducible amplification of single-copy genomic DNA (gDNA) starting with 1–10 cells or equivalent picogram quantities of isolated gDNA. Cell lysis and whole-genome preamplification are followed by amplification, which results in libraries with very low background and typical yields of 8–12 µg of product in under 3 hours. This system is best suited for applications in which high yield is most important. This product contains reagents for 96 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data
R300673 PicoPLEX® Single Cell WGA Kit 480 Rxns USD $9448.00

The PicoPLEX Single Cell WGA Kit uses a single-tube protocol developed specifically for reproducible amplification of single-copy genomic DNA (gDNA) starting with 1–10 cells or equivalent picogram quantities of isolated gDNA. Cell lysis and whole-genome preamplification are followed by amplification, which results in libraries with very low background and typical yields of 8–12 µg of product in under 3 hours. This product is composed of five 96-reaction kits (Cat. # R300672), 480 reactions total, of the PicoPLEX Single Cell WGA reagents.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data
R300718 PicoPLEX® Single Cell WGA Kit v3 24 Rxns USD $590.00

The PicoPLEX Single Cell WGA Kit v3 uses a single-tube protocol developed specifically for the reproducible amplification of genomic DNA (gDNA) starting from 1–10 cells or equivalent picogram quantities of isolated gDNA. Cell lysis and whole-genome preamplification are followed by high-fidelity amplification with very low background to yield over 2 µg of product in under 3 hours. This kit is suited for applications in which base-level resolution is required, such as for the detection of mutations. This product contains reagents for 24 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data
R300722 PicoPLEX® Single Cell WGA Kit v3 96 Rxns USD $2097.00

The PicoPLEX Single Cell WGA Kit v3 uses a single-tube protocol developed specifically for the reproducible amplification of genomic DNA (gDNA) starting from 1–10 cells or equivalent picogram quantities of isolated gDNA. Cell lysis and whole-genome preamplification are followed by high-fidelity amplification with very low background to yield over 2 µg of product in under 3 hours. This kit is suited for applications in which base-level resolution is required, such as for the detection of mutations. This product contains reagents for 96 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data
R300723 PicoPLEX® Single Cell WGA Kit v3 480 Rxns USD $8907.00

The PicoPLEX Single Cell WGA Kit v3 uses a single-tube protocol developed specifically for the reproducible amplification of genomic DNA (gDNA) starting from 1–10 cells or equivalent picogram quantities of isolated gDNA cell lysis and whole-genome preamplification are followed by high-fidelity amplification with very low background to yield over 2 µg of product in under 3 hours. This kit is suited for applications in which base-level resolution is required, such as for the detection of mutations. This product is composed of five 96-reaction kits (Cat. # R300722), 480 reactions total, of the PicoPLEX Single Cell WGA v3 reagents.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data
R30050 PicoPLEX® WGA Kit 50 Rxns USD $1249.00

The PicoPLEX WGA Kit uses a single-tube protocol developed specifically for the reproducible amplification of genomic DNA (gDNA) starting from 1–10 cells or equivalent picogram quantities of isolated gDNA. Cell lysis and whole-genome preamplification are followed by amplification with very low background to yield over 2 µg of product in under 3 hours. This is the kit of choice for assays that need the most accurate and reproducible measurement of CNV. This product contains reagents for 50 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Overview

  • Reproducible results from single cells (or 6–50 pg of DNA)
  • Superior reproducibility of allele representation
  • Easy to use and automate
  • Unambiguous results at all resolutions (e.g., microarray and PCR)

More Information

Applications

  • CNV analysis using NGS, oligonucleotide aCGH, BAC aCGH, and qPCR
  • Target enrichment or amplicon enrichment for mutation detection
  • SNP genotyping

Suitable Inputs

  • Mammalian cells (1–10 cells)
  • Bacterial cells (1,000–10,000 cells)
  • Isolated DNA (6–50 pg of human DNA)
  • Sorted chromosomes
  • Intact or fragmented, single- or double-stranded DNA

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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