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  • ‹ Back to Epigenetics and small RNA sequencing
  • ChIP-seq library construction
  • Meth-seq kit
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Home › Products › Next-generation sequencing › Epigenetics and small RNA sequencing › Meth-seq kit

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Technical notes
View data for this product

EpiXplore Meth-Seq DNA Enrichment Kit

The EpiXplore Meth-Seq DNA Enrichment Kit is a complete system for separating methylated and unmethylated fractions of sheared genomic DNA and generating sequencing-ready Illumina libraries from the enriched DNA fractions. The enrichment protocol utilizes the his-tagged MBD2 protein currently used in the EpiXplore Methylated DNA Enrichment Kit in conjunction with Capturem Nanoprep Columns to separate methylated DNA from unmethylated DNA in ~2 hours. Illumina-compatible sequencing libraries are then generated from the methylated (or unmethylated, if desired) DNA fractions using DNA SMART technology.

For library prep, the DNA SMART method has been adapted for the addition of Illumina-specific sequencing adapters to the ends of DNA fragments in a ligation-independent manner. This highly sensitive technology generates robust sequencing libraries from low-input DNA samples, with minimal handling, in about four hours.

Cat. # Product Size License Quantity Details
635023 EpiXplore™ Meth-Seq DNA Enrichment Kit 12 Rxns USD $1171.00

License Statement

ID Number  
273 This product is covered by U.S. Patent Nos. 9,459,188 and/or 10,207,229, exclusively licensed to Takara Bio USA, Inc.
270 This product is covered by U.S. Patent No. 9,410,173 and other pending patent applications in the United States and foreign jurisdictions. For further information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com.
347 This product is protected by U.S. Patents 9,895,665, 10,195,569 and foreign counterparts and/or additional U.S. and foreign patents pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

The EpiXplore Meth-Seq DNA Enrichment Kit is a methylated DNA enrichment and library prep kit. This is a product for separating the methylated and unmethylated fractions of DNA, and generating sequencing-ready Illumina® libraries from sheared genomic DNA. This kit utilizes his-tagged MBD2 protein and CapturemTM Nanoprep columns to separate methylated DNA from unmethylated DNA in ~2 hours, and employs our DNA SMARTTM technology to generate indexed meth-seq libraries suitable for NGS on Illumina sequencing platforms. Sequencing libraries are generated without the need for enzymatic cleanup or additional adapter ligations. All necessary reagents for producing meth-seq libraries are provided in this kit, including primer sets that contain Illumina index sequences. For more information on the indexes supplied in each kit, please refer to the relevant Certificates of Analysis.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

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Flowchart for separating methylated and unmethylated fractions of DNA

Flowchart for separating methylated and unmethylated fractions of DNA
Flowchart for separating methylated and unmethylated fractions of DNA. In the enrichment portion of the protocol, sheared DNA is incubated with his-tagged MBD2 protein for 1 hr, which allows the methylated DNA fraction to bind to the protein. The solution is then added to the Capturem nanoprep column, which binds the his-tagged protein and thus the methylated DNA. Subsequent washes with the Unmethylated Elution Buffer remove the unmethylated DNA. The methylated DNA is then eluted in a high-salt buffer. After cleanup, this enriched DNA can be used as the input for library preparation.

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Flowchart for DNA-SMART ChIP-Seq technology

Flowchart for DNA-SMART ChIP-Seq technology
Flowchart for DNA-SMART ChIP-Seq technology. This single-tube workflow allows users to generate Illumina-compatible libraries for meth-seq experiments. After library size selection and purification, the total time from input DNA to meth-seq library is approximately four hours. DNA SMART technology eliminates the need for an adapter ligation step and associated cleanup, reducing the loss of limited input DNA.

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Distinct methylation patterns are observed in aligned NGS data of libraries generated from methylated and unmethylated DNA fractions

Distinct methylation patterns are observed in aligned NGS data of libraries generated from methylated and unmethylated DNA fractions
Distinct methylation patterns are observed in aligned NGS data of libraries generated from methylated and unmethylated DNA fractions. A representative region of Arabidopsis chromosome 3 (outlined in the green box) shows the peaks identified in the DNA enriched for methylated and unmethylated fractions. The methylated regions are outlined in red boxes and the unmethylated regions are outlined in blue boxes. The amount of initial input DNA is shown along the left-hand side of the alignments. The data show good concordance with the publicly available bisulfite sequencing data. Data were visualized using the Integrative Genomics Viewer (Broad Institute). Public data was obtained from GEO accession GSM980986; Stroud et al., 2013.

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635023: EpiXplore Meth-Seq DNA Enrichment Kit

635023: EpiXplore Meth-Seq DNA Enrichment Kit

Overview

  • Rapid, user-friendly protocol which separates methylated DNA from unmethylated DNA
  • Ligation-independent sequencing-adapter addition for library preparation
  • DNA inputs of 25 ng–1 μg sheared genomic DNA
  • Generate Illumina-ready sequencing libraries from the enriched DNA in ~4 hours

More Information

Applications

  • Sequencing library preparation for methylated DNA-seq from low-input, sheared genomic DNA
  • Illumina-specific NGS sequencing library production

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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