All-purpose library prep for fragmented DNA from any sample type

The ThruPLEX DNA-Seq Kit enables generation of high-quality DNA libraries from as little as 50 pg of input material, and features a user-friendly protocol that is as simple as any NGS library prep solution currently available on the market. This kit can be used to process fragmented double-stranded DNA (dsDNA) from any sample source, including genomic DNA isolated from cells/tissues, cell-free DNA, FFPE DNA, outputs from ChIP or CUT&RUN approaches, and RNA-derived cDNA.

The entire ThruPLEX DNA-Seq Kit workflow is performed in a single tube or well in about two hours and requires no intermediate purification steps or sample transfers that pose the risk of sample loss and reduced library complexity. ThruPLEX technology enables efficient repair and capture of dsDNA fragments as small as 50 bp and has been successfully paired with commonly used target enrichment platforms, allowing for a wide range of NGS-based applications. Compatible indexing reagents are described below and allow for multiplexing of up to 384 samples per sequencing run.

Unique Dual Index kits (plate format)

Unique Dual Index kits are designed for use with the vast majority of NGS library preparation kits from Takara Bio for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers that include the same adapter as the IDT for Illumina-TruSeq DNA and RNA UD Indexes, with Cat. # 634752 and 634756 carrying the “IDT for Illumina” indexes and Cat. # 634753–634755 carrying indexes that have been developed and validated by Takara Bio. These kits offer a total of 384 dual indexes for multiplexing of up to 384 samples. The indexed PCR primers are supplied pre-dispensed in five different 96-well plate formats, four of which contain one-fourth of the possible dual index combinations (Cat. # 634752–634755) with the remaining kit (Cat. # 634756) containing 24 dual index combinations that are a subset of Cat. # 634752. Each dual index combination is provided at a volume of 5 µl and intended for a single use.

DNA Unique Dual Index kits (tube format)

DNA Unique Dual Index kits are designed for use with ThruPLEX and PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the "IDT for Illumina UD" index sequences and offer a total of 96 dual indexes for multiplexing of up to 96 samples. The indexed PCR primers are supplied pre-dispensed in four different sets of 24 individual tubes, each of which contains one-fourth of the possible dual index combinations (Cat. # R400665–R400668). Each dual index tube (24U Sets A–D) contains sufficient volume for up to two uses.

DNA Single Index kits (tube format)

DNA Single Index kits are designed for use with ThruPLEX and PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the "TruSeq LT set A" index sequences and offer a total of 24 single indexes for multiplexing of up to 24 samples. The indexed PCR primers are supplied pre-dispensed in two different sets of 12 individual tubes (Cat. # R400695 and R400697), each containing a different index sequence. Each single index tube contains sufficient volume for up to eight uses.