SMARTer ThruPLEX DNA-Seq Kit: all-purpose DNA library preparation for Illumina NGS platforms
The SMARTer ThruPLEX DNA-Seq Kit builds on innovative ThruPLEX chemistry to generate DNA libraries from as little as 50 pg of DNA, and improves sensitivity while providing up to 384 indexes for multiplexing. This kit can be used with fragmented double-stranded DNA (dsDNA) from any sample source—genomic DNA, biofluids such as cell-free DNA (cfDNA), DNA from FFPE materials, and cDNA.
The SMARTer ThruPLEX DNA-Seq Kit has been reconfigured from the original versions of ThruPLEX kits and does not include indexes. Compatible SMARTer Index kits are described below and can be purchased here.
The SMARTer ThruPLEX DNA-Seq Kit builds on innovative ThruPLEX chemistry to generate DNA libraries from as little as 50 pg of DNA, and improves sensitivity while providing up to 384 indexes for multiplexing. This kit can be used with fragmented double-stranded DNA (dsDNA) from any sample source—genomic DNA, biofluids such as cell-free DNA (cfDNA), DNA from FFPE materials, and cDNA.
The SMARTer ThruPLEX DNA-Seq Kit has been reconfigured from the original versions of ThruPLEX kits and does not include indexes. Compatible SMARTer Index kits are described below and can be purchased here.
The entire SMARTer ThruPLEX DNA-Seq Kit workflow is performed in a single tube or well in about two hours and requires no purification steps or sample transfers. The kit can be used in any DNA-seq application, RNA-seq, or ChIP-seq and offers robust target enrichment performance with all of the leading platforms.
SMARTer DNA HT Dual Index kits
SMARTer DNA HT Dual Index kits are designed for use with SMARTer ThruPLEX and SMARTer PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the Illumina Nextera® XT v2 index sequences and offer a total of 384 dual indexes for multiplexing of up to 384 samples. The indexed PCR primers are supplied pre-dispensed in four different barcoded index plates, each of which contains one-fourth of the possible dual index combinations (Cat. # R400660–R400663); or in a set of 24 individual tubes, each containing a different dual index combination (Cat. # R400664). Each dual index tube (24N) contains sufficient volume for up to two uses. Each well of a dual index plate (96N Sets A–D) contains sufficient volume for a single use.
SMARTer DNA Unique Dual Index kits
SMARTer DNA Unique Dual Index kits are designed for use with SMARTer ThruPLEX and SMARTer PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the "IDT for Illumina UD" index sequences and offer a total of 96 dual indexes for multiplexing of up to 96 samples. The indexed PCR primers are supplied pre-dispensed in four different sets of 24 individual tubes, each of which contains one-fourth of the possible dual index combinations (Cat. Nos. R400665–R400668). Each dual index tube (24U Sets A–D) contains sufficient volume for up to two uses.
SMARTer DNA Single Index kits
SMARTer DNA Single Index kits are designed for use with SMARTer ThruPLEX and SMARTer PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the "TruSeq LT set A" index sequences and offer a total of 12 single indexes for multiplexing of up to 12 samples. The indexed PCR primers are supplied pre-dispensed in four different sets of 12 individual tubes, each containing a different index sequence (Cat. Nos. R400695 and R400697). Each single index tube (12S Sets A–B) contains sufficient volume for up to eight uses.
Overview
- High performance from low inputs—generates libraries from 50 pg to 50 ng of input material
- Fast and simple workflow—three-step, single-tube or single-well protocol takes only about two hours, no transfers necessary
- Compatible with major target enrichment platforms—suitable for use with Agilent SureSelect, Roche Nimblegen SeqCap EZ, and IDT xGen Lockdown probes
More Information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
See what our customers are saying about ThruPLEX DNA-seq technology!
"We are very happy with the results and we plan to start using ThruPLEX DNA-seq as a part of our capture workflows. Variants are called with very equal distribution (only a small fraction of low/high coverage extremes). In our classic sample preps, the coverage is not this homogenous, and low tail is much longer. Our lab staff was pleased with the convenience of the ThruPLEX workflow (less manual steps)."
—Pekka Ellonen, Head of DNA Sequencing Laboratory, INSTITUTE FOR MOLECULAR MEDICINE FINLAND FIMM
DNA-seq protocols:
User-generated DNA target enrichment protocols for SMARTer ThruPLEX kits
Currently featuring protocols for integrating SMARTer ThruPLEX kits with leading target enrichment systems.
Epigenetics citations
A list of publications using ThruPLEX DNA-seq kits and SMARTer RNA-seq kits to perform ChIP-seq, CUT&RUN, and smRNA-seq epigenetics studies.
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