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  • ‹ Back to DNA-seq
  • Low-input library prep for Illumina
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Customer spotlight: overcoming the limits of ChIP-seq with CUT&RUN sequencing Blog post: CUT&RUN customer spotlight
Technical notes
DNA-seq from FFPE samples
Technical notes
View data on low cell number ChIP-seq
Technical notes
View data on shearing low-volume DNA samples
Technical notes
View data on low-input whole-exome sequencing
Home › Products › Next-generation sequencing › DNA-seq › Low-input library prep for Illumina

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Need help?
Customer spotlight: overcoming the limits of ChIP-seq with CUT&RUN sequencing Blog post: CUT&RUN customer spotlight
Technical notes
DNA-seq from FFPE samples
Technical notes
View data on low cell number ChIP-seq
Technical notes
View data on shearing low-volume DNA samples
Technical notes
View data on low-input whole-exome sequencing

SMARTer ThruPLEX DNA-Seq Kit: all-purpose DNA library preparation for Illumina NGS platforms

SMARTer ThruPLEX DNA-Seq Kit product

The SMARTer ThruPLEX DNA-Seq Kit builds on innovative ThruPLEX chemistry to generate DNA libraries from as little as 50 pg of DNA, and improves sensitivity while providing up to 384 indexes for multiplexing. This kit can be used with fragmented double-stranded DNA (dsDNA) from any sample source—genomic DNA, biofluids such as cell-free DNA (cfDNA), DNA from FFPE materials, and cDNA.

The SMARTer ThruPLEX DNA-Seq Kit has been reconfigured from the original versions of ThruPLEX kits and does not include indexes. Compatible SMARTer Index kits are described below and can be purchased here.

The SMARTer ThruPLEX DNA-Seq Kit builds on innovative ThruPLEX chemistry to generate DNA libraries from as little as 50 pg of DNA, and improves sensitivity while providing up to 384 indexes for multiplexing. This kit can be used with fragmented double-stranded DNA (dsDNA) from any sample source—genomic DNA, biofluids such as cell-free DNA (cfDNA), DNA from FFPE materials, and cDNA.

The SMARTer ThruPLEX DNA-Seq Kit has been reconfigured from the original versions of ThruPLEX kits and does not include indexes. Compatible SMARTer Index kits are described below and can be purchased here.

The entire SMARTer ThruPLEX DNA-Seq Kit workflow is performed in a single tube or well in about two hours and requires no purification steps or sample transfers. The kit can be used in any DNA-seq application, RNA-seq, or ChIP-seq and offers robust target enrichment performance with all of the leading platforms.

SMARTer DNA HT Dual Index kits

SMARTer DNA HT Dual Index kits are designed for use with SMARTer ThruPLEX and SMARTer PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the Illumina Nextera® XT v2 index sequences and offer a total of 384 dual indexes for multiplexing of up to 384 samples. The indexed PCR primers are supplied pre-dispensed in four different barcoded index plates, each of which contains one-fourth of the possible dual index combinations (Cat. # R400660–R400663); or in a set of 24 individual tubes, each containing a different dual index combination (Cat. # R400664). Each dual index tube (24N) contains sufficient volume for up to two uses. Each well of a dual index plate (96N Sets A–D) contains sufficient volume for a single use.

SMARTer DNA Unique Dual Index kits

SMARTer DNA Unique Dual Index kits are designed for use with SMARTer ThruPLEX and SMARTer PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the "IDT for Illumina UD" index sequences and offer a total of 96 dual indexes for multiplexing of up to 96 samples. The indexed PCR primers are supplied pre-dispensed in four different sets of 24 individual tubes, each of which contains one-fourth of the possible dual index combinations (Cat. Nos. R400665–R400668). Each dual index tube (24U Sets A–D) contains sufficient volume for up to two uses.

SMARTer DNA Single Index kits

SMARTer DNA Single Index kits are designed for use with SMARTer ThruPLEX and SMARTer PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the "TruSeq LT set A" index sequences and offer a total of 12 single indexes for multiplexing of up to 12 samples. The indexed PCR primers are supplied pre-dispensed in four different sets of 12 individual tubes, each containing a different index sequence (Cat. Nos. R400695 and R400697). Each single index tube (12S Sets A–B) contains sufficient volume for up to eight uses.

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Cat. # Product Size Price License Quantity Details
R400674 SMARTer® ThruPLEX® DNA-Seq Kit 24 Rxns $597.00

License Statement

ID Number  
326 This product is protected by U.S. Patents 7,803,550; 8,071,312; 8,399,199; 8,728,737 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
M54 This product is covered by the claims of U.S. Patent Nos. 7,704,713 and its foreign counterparts. 

The SMARTer ThruPLEX DNA-Seq Kit builds on the innovative ThruPLEX chemistry to generate high-complexity DNA libraries from as little as 50 pg of DNA. Single index, dual index, and unique dual index kits are available and must be purchased separately. This product contains reagents for 24 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components
R400675 SMARTer® ThruPLEX® DNA-Seq Kit 48 Rxns $1152.00

License Statement

ID Number  
M54 This product is covered by the claims of U.S. Patent Nos. 7,704,713 and its foreign counterparts. 
326 This product is protected by U.S. Patents 7,803,550; 8,071,312; 8,399,199; 8,728,737 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

The SMARTer ThruPLEX DNA-Seq Kit builds on the innovative ThruPLEX chemistry to generate high-complexity DNA libraries from as little as 50 pg of DNA. Single index, dual index, and unique dual index kits are available and must be purchased separately. This product contains reagents for 48 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components
R400676 SMARTer® ThruPLEX® DNA-Seq Kit 96 Rxns $2112.00

License Statement

ID Number  
M54 This product is covered by the claims of U.S. Patent Nos. 7,704,713 and its foreign counterparts. 
326 This product is protected by U.S. Patents 7,803,550; 8,071,312; 8,399,199; 8,728,737 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

The SMARTer ThruPLEX DNA-Seq Kit builds on the innovative ThruPLEX chemistry to generate high-complexity DNA libraries from as little as 50 pg of DNA. Single index, dual index, and unique dual index kits are available and must be purchased separately. This product contains reagents for 96 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components
R400677 SMARTer® ThruPLEX® DNA-Seq Kit 480 Rxns $9360.00

License Statement

ID Number  
M54 This product is covered by the claims of U.S. Patent Nos. 7,704,713 and its foreign counterparts. 
326 This product is protected by U.S. Patents 7,803,550; 8,071,312; 8,399,199; 8,728,737 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

The SMARTer ThruPLEX DNA-Seq Kit builds on the innovative ThruPLEX chemistry to generate high-complexity DNA libraries from as little as 50 pg of DNA. Single index, dual index, and unique dual index kits are available and must be purchased separately. This product is composed of five 96-reaction kits (Cat. No. R400676), 480 reactions total, of SMARTer ThruPLEX DNA-Seq reagents.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components

SMARTer DNA index kits SMARTer DNA index kits (required)

Overview

  • High performance from low inputs—generates libraries from 50 pg to 50 ng of input material
  • Fast and simple workflow—three-step, single-tube or single-well protocol takes only about two hours, no transfers necessary
  • Compatible with major target enrichment platforms—suitable for use with Agilent SureSelect, Roche Nimblegen SeqCap EZ, and IDT xGen Lockdown probes

More Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


See what our customers are saying about SMARTer ThruPLEX DNA-seq technology!

"We are very happy with the results and we plan to start using ThruPLEX DNA-seq as a part of our capture workflows. Variants are called with very equal distribution (only a small fraction of low/high coverage extremes). In our classic sample preps, the coverage is not this homogenous, and low tail is much longer. Our lab staff was pleased with the convenience of the ThruPLEX workflow (less manual steps)."
—Pekka Ellonen, Head of DNA Sequencing Laboratory, INSTITUTE FOR MOLECULAR MEDICINE FINLAND FIMM

DNA-seq protocols:

DNA target enrichment protocols for SMARTer ThruPLEX kits

Currently featuring protocols for integrating SMARTer ThruPLEX kits with leading target enrichment systems.

Target enrichment with SMARTer ThruPLEX NGS libraries


Epigenetics citations

A list of publications using SMARTer RNA-seq and DNA-seq kits to perform epigenetic analyses, including smRNA-seq and ChIP-seq.

View smRNA-seq and ChIP-seq citations

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

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Capturem Trypsin for a rapid, efficient mass spectometry workflow at room temperature.

Speed up your mass spec workflow

Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis. This product utilizes our novel Capturem technology in a spin column format with membrane-immobilized trypsin. Capturem Trypsin Columns may be used to completely digest protein samples in less than a minute with digestion efficiencies (protein coverage) comparable to or better than those obtained using in-solution trypsin digestion.

Capturem trypsin technology

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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