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  • ‹ Back to DNA-seq
  • NGS library prep with enzymatic fragmentation
  • Comparing ThruPLEX FLEX EF to Kapa and NEBNext
  • Next-gen WGA method for CNV and SNV detection from single cells
  • Low-input whole-exome sequencing
  • DNA-seq from FFPE samples
  • Low cell number ChIP-seq using ThruPLEX DNA-Seq
  • Detection of low-frequency variants using ThruPLEX Tag-Seq FLEX
  • ThruPLEX FLEX outperforms NEBNext Ultra II
  • Streamlined DNA-seq from challenging samples
  • High-resolution CNV detection using PicoPLEX Gold DNA-Seq
  • ThruPLEX FLEX data sheet
  • Low-volume DNA shearing for ThruPLEX library prep
ThruPLEX Tag-seq and DNA-seq FLEX product page ThruPLEX FLEX product information
ThruPLEX DNA-seq FLEX tech note View DNA-Seq FLEX data
ThruPLEX Tag-Seq FLEX tech note View Tag-Seq FLEX data
ThruPLEX FLEX benchmarking tech note Benchmarking ThruPLEX FLEX vs. NEBNext
Home › Learning centers › Next-generation sequencing › Technical notes › DNA-seq › ThruPLEX FLEX data sheet

Technical notes

  • DNA-seq
    • NGS library prep with enzymatic fragmentation
    • Comparing ThruPLEX FLEX EF to Kapa and NEBNext
    • Next-gen WGA method for CNV and SNV detection from single cells
    • Low-input whole-exome sequencing
    • DNA-seq from FFPE samples
    • Low cell number ChIP-seq using ThruPLEX DNA-Seq
    • Detection of low-frequency variants using ThruPLEX Tag-Seq FLEX
    • ThruPLEX FLEX outperforms NEBNext Ultra II
    • Streamlined DNA-seq from challenging samples
    • High-resolution CNV detection using PicoPLEX Gold DNA-Seq
    • ThruPLEX FLEX data sheet
    • Low-volume DNA shearing for ThruPLEX library prep
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ThruPLEX Tag-seq and DNA-seq FLEX product page ThruPLEX FLEX product information
ThruPLEX DNA-seq FLEX tech note View DNA-Seq FLEX data
ThruPLEX Tag-Seq FLEX tech note View Tag-Seq FLEX data
ThruPLEX FLEX benchmarking tech note Benchmarking ThruPLEX FLEX vs. NEBNext

ThruPLEX FLEX: representative data from difficult samples

Note: The protocols and QC procedures for ThruPLEX HV kits have been updated to accommodate lower inputs and compatibility with the Unique Dual Index Kit sets. While product naming has been revised accordingly (ThruPLEX FLEX), reagent formulations remain unchanged.

  • Streamlined workflow with high reproducibility
  • Robust performance with FFPE and cfDNA
  • Accurate detection of rare mutations with molecular tags
  • Coverage for a broad range of GC content with microbiome samples
  • Unique dual indexes for use on the NovaSeq™ system

Simple workflow: one tube and done

Addition-only steps provide a faster, failproof library prep experience without lengthy bead purifications that cause sample loss and impact data quality. Without hidden steps—like adapter dilution—it is easy to automate, scale, and standardize. Pre-plated, single-use UDI indexes make sample assignment clear for your precious materials. Learn more »

ThurPLEX HV workflow

Figure 1. ThruPLEX FLEX chemistry features a fast, simple workflow without bead purification after ligation—saving time, money, and resources


Reproducible high-quality data from FFPE

Formalin-fixed paraffin-embedded samples are a gold mine of data for understanding disease—if you are equipped to tackle these challenging samples. ThruPLEX FLEX kits have been optimized to produce high-quality, reproducible data from FFPE tissue. View data »

ThruPLEX HV DNA-seq data

Figure 2. Expect highly reproducible and consistent performance at a broad range of inputs, even for highly degraded FFPE DNA.


Reliably detect low-frequency mutations from cfDNA with molecular tags

Cell-free samples, such as plasma, pose significant challenges to scientists interested in using minimally invasive techniques to look at DNA—especially when the biomarkers of interest are rare. ThruPLEX Tag-Seq FLEX provides an elegant solution, with chemistry designed to generate complex libraries and molecular tags that enable accurate detection of rare mutations. View data »

ThruPLEX HV molecular tags

50-ng input 10-ng input
Gene Nucleotide Amino acid Cosmic ID WT WT 5% 5% 1% 1% WT WT 5% 5% 1% 1%
EGFR 2573T>G L858R COSM6224 0.0% 0.0% 6.5% 5.2% 1.3% 1.5% 0.0% 0.0% 5.7% 5.8% 1.3% 2.1%
EGFR 2369C>T T790M COSM6240 0.0% 0.0% 2.6% 5.6% 0.4% 1.3% 0.0% 0.0% 2.4% 1.9% 1.5% 1.9%
KRAS 35G>A G12D COSM521 0.0% 0.0% 4.0% 3.8% 1.3% 1.6% 0.0% 0.0% 3.5% 3.6% 1.5% 0.5%
NRAS 181C>A Q61K COSM580 0.0% 0.0% 8.6% 5.1% 2.4% 1.7% 0.0% 0.0% 6.4% 5.1% 2.5% 2.3%
PIK3CA 1633G>A E545K COSM763 0.0% 0.0% 4.2% 3.8% 0.9% 1.3% 0.0% 0.0% 4.2% 5.7% miss 1.5%

Figure 3. Accurately detect low-frequency mutations with the ThruPLEX Tag-Seq FLEX system, featuring discrete, balanced molecular tags.


Related products

Cat. # Product Size Price License Quantity Details
R400737 ThruPLEX® DNA-Seq FLEX 24 Rxns USD $657.00

License Statement

ID Number  
326 This product is protected by U.S. Patents 7,803,550; 8,399,199; 8,728,737, 9,598,727, 10,196,686, 10,208,337, 11,072,823 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

ThruPLEX DNA-Seq FLEX uses a simple, three-step workflow to generate high-complexity DNA libraries from standard or challenging samples such as FFPE and cell-free DNA. Unique dual index (UDI) kits are available for purchase separately. This product contains reagents for 24 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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R400737: ThruPLEX DNA-Seq HV Core Components

R400737: ThruPLEX DNA-Seq HV Core Components
R400736 ThruPLEX® DNA-Seq FLEX 96 Rxns USD $2353.00

License Statement

ID Number  
326 This product is protected by U.S. Patents 7,803,550; 8,399,199; 8,728,737, 9,598,727, 10,196,686, 10,208,337, 11,072,823 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

ThruPLEX DNA-Seq FLEX uses a simple, three-step workflow to generate high-complexity DNA libraries from standard or challenging samples such as FFPE and cell-free DNA. Unique dual index (UDI) kits are available for purchase separately. This product contains reagents for 96 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

R400736: ThruPLEX DNA-Seq HV Core Components

R400736: ThruPLEX DNA-Seq HV Core Components
R400734 ThruPLEX® Tag-Seq FLEX 24 Rxns USD $1059.00

License Statement

ID Number  
384 This product is protected by U.S. Patents 7,803,550, 8,399,199; 9,598,727, 10,196,686, 10,208,337, and 10,155,942 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
438 This Product is protected by one or more patents from the family comprising:US10155942, AU2013337280, CA2889862, People's Republic of China Patent: ZL201380069090.3, US10961529, DE602013026292.6, EP2914745, UK2914745, HK1089485, JP6454281 and any corresponding patents, divisionals, continuations, patent application and foreign filings sharing priority with the same family.
448 This product is sold under license from Becton Dickinson and Company and is covered by one or more of the following US Patent Nos. 8,835,358; 9,290,808; 9,290,809; 9,315,857; 9,708,659; 9,816,137; 9,845,502; 10,047,394; 10,059,991; 10,202,646; 10,392,661; 10,619,203; and pending U.S. patent applications 16/551,638 and 16/846,133.

ThruPLEX Tag-Seq FLEX uses a simple, three-step workflow to generate high-complexity DNA libraries with unique molecular tags from standard or challenging samples such as FFPE and cell-free DNA. Unique dual index (UDI) kits are available for purchase separately. This product contains reagents for 24 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

R400734: ThruPLEX Tag-Seq HV Core Components

R400734: ThruPLEX Tag-Seq HV Core Components
R400735 ThruPLEX® Tag-Seq FLEX 96 Rxns USD $3740.00

License Statement

ID Number  
384 This product is protected by U.S. Patents 7,803,550, 8,399,199; 9,598,727, 10,196,686, 10,208,337, and 10,155,942 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
438 This Product is protected by one or more patents from the family comprising:US10155942, AU2013337280, CA2889862, People's Republic of China Patent: ZL201380069090.3, US10961529, DE602013026292.6, EP2914745, UK2914745, HK1089485, JP6454281 and any corresponding patents, divisionals, continuations, patent application and foreign filings sharing priority with the same family.
448 This product is sold under license from Becton Dickinson and Company and is covered by one or more of the following US Patent Nos. 8,835,358; 9,290,808; 9,290,809; 9,315,857; 9,708,659; 9,816,137; 9,845,502; 10,047,394; 10,059,991; 10,202,646; 10,392,661; 10,619,203; and pending U.S. patent applications 16/551,638 and 16/846,133.

ThruPLEX Tag-Seq FLEX uses a simple, three-step workflow to generate high-complexity DNA libraries with unique molecular tags from standard or challenging samples such as FFPE and cell-free DNA. Unique dual index (UDI) kits are available for purchase separately. This product contains reagents for 96 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

R400735: ThruPLEX Tag-Seq HV Core Components

R400735: ThruPLEX Tag-Seq HV Core Components

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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