ThruPLEX HV: reliable, consistent data from difficult samples
- Streamlined workflow with high reproducibility
- Robust performance with FFPE and cfDNA
- Accurate detection of rare mutations with molecular tags
- Coverage for a broad range of GC content with microbiome samples
- Unique dual indexes for use on the NovaSeq™ system
Simple workflow: one tube and done
Addition-only steps provide a faster, failproof library prep experience without lengthy bead purifications that cause sample loss and impact data quality. Without hidden steps—like adapter dilution—it is easy to automate, scale, and standardize. Pre-plated, single-use UDI indexes make sample assignment clear for your precious materials. Learn more »
Figure 1. ThruPLEX HV chemistry features a fast, simple workflow without bead purification after ligation—saving time, money, and resources
Reproducible high-quality data from FFPE
Formalin-fixed paraffin-embedded samples are a gold mine of data for understanding disease—if you are equipped to tackle these challenging samples. ThruPLEX HV kits have been optimized to produce high-quality, reproducible data from FFPE tissue. View data »
Figure 2. Expect highly reproducible and consistent performance at a broad range of inputs, even for highly degraded FFPE DNA.
Reliably detect low-frequency mutations from cfDNA with molecular tags
Cell-free samples, such as plasma, pose significant challenges to scientists interested in using minimally invasive techniques to look at DNA—especially when the biomarkers of interest are rare. ThruPLEX Tag-Seq HV provides an elegant solution, with chemistry designed to generate complex libraries and molecular tags that enable accurate detection of rare mutations. View data »
| 50-ng input | 10-ng input | ||||||||||||||
| Gene | Nucleotide | Amino acid | Cosmic ID | WT | WT | 5% | 5% | 1% | 1% | WT | WT | 5% | 5% | 1% | 1% |
| EGFR | 2573T>G | L858R | COSM6224 | 0.0% | 0.0% | 6.5% | 5.2% | 1.3% | 1.5% | 0.0% | 0.0% | 5.7% | 5.8% | 1.3% | 2.1% |
| EGFR | 2369C>T | T790M | COSM6240 | 0.0% | 0.0% | 2.6% | 5.6% | 0.4% | 1.3% | 0.0% | 0.0% | 2.4% | 1.9% | 1.5% | 1.9% |
| KRAS | 35G>A | G12D | COSM521 | 0.0% | 0.0% | 4.0% | 3.8% | 1.3% | 1.6% | 0.0% | 0.0% | 3.5% | 3.6% | 1.5% | 0.5% |
| NRAS | 181C>A | Q61K | COSM580 | 0.0% | 0.0% | 8.6% | 5.1% | 2.4% | 1.7% | 0.0% | 0.0% | 6.4% | 5.1% | 2.5% | 2.3% |
| PIK3CA | 1633G>A | E545K | COSM763 | 0.0% | 0.0% | 4.2% | 3.8% | 0.9% | 1.3% | 0.0% | 0.0% | 4.2% | 5.7% | miss | 1.5% |
Figure 3. Accurately detect low-frequency mutations with the ThruPLEX Tag-Seq HV system, featuring discrete, balanced molecular tags.
Related products (visit takarabio.com/thruplex-hv-products for additional configurations)
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