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Adeno-X adenoviral system 3

Adeno-X System 3, the most advanced adenoviral vector system

Adeno-X adenoviral system 3 is the most advanced commercially available adenoviral gene-delivery system—providing by far the simplest, fastest, and most efficient method for constructing recombinant adenoviral vectors. Seven formats are available, including the most advanced tetracycline-inducible expression system, constitutive expression systems with or without fluorescent reporters, and universal (promoterless) systems that allow you to clone and express any entire expression cassette of your choice.

Adeno-X adenoviral system 3 is the most advanced commercially available adenoviral gene-delivery system—providing the simplest, fastest, and most efficient method for constructing recombinant adenoviral vectors. Seven formats are available, including the most advanced tetracycline-inducible expression system, constitutive expression systems with a choice of fluorescent reporters, and promoterless systems that allow you to clone and express any entire expression cassette of your choice.

There is no simpler adenoviral expression system

The system relies on the ability of the In-Fusion HD enzyme to precisely recognize and fuse 15 bp of homology between two linear DNA molecules. To generate short regions of homology to the prelinearized pAdeno-X vector, simply add 15 bp of additional sequence to the primers you use to PCR amplify your gene of interest. Then, combine the DNA together with the In-Fusion HD enzyme in a 15-min reaction and transform Stellar Competent Cells. Cloning is always directional, and 90% of the clones contain the correct insert. The kit includes a control cloning fragment, primers for colony PCR verification of positive clones, and a NucleoBond Xtra kit for transfection-grade plasmid purification.

Tetracycline-inducible expression

When you clone your gene into pAdenoX-Tet3G, you are creating a system with the tightest and most sensitive control of gene expression. Tightly controlled, doxycycline-induced expression is as easy as constitutive expression since the Tet-On 3G transactivator protein and the P_TRE3G controlled gene of interest are contained on the same adenoviral vector. Up to 3,000-fold induction can be achieved using this system.

Clone any expression cassette into the universal vectors

You are not limited to using a CMV expression system—we have created universal adenoviral systems with vectors that lack a promoter and polyA signal in the cloning site. Simply amplify an entire expression cassette (from promoter to polyA) from a pre-existing construct and clone using In-Fusion HD. Universal systems can be used for expression from alternative promoters that are more suitable to your target cell type such as EF1 alpha or tissue-specific promoters.

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Cat. #

Product

Size

Price

License

Quantity

Details

632269

Adeno-X™ Adenoviral System 3 (CMV)

10 Rxns

$1092.00

License Statement

ID Number
28	This product is covered by the claims of U.S. Patent No. 7,575,860 and its foreign counterpart patent claims.
25	This product is covered by U.S. Patent No. 6,303,362.

Adeno-X Adenoviral System 3 (CMV) provides the components necessary to generate a constitutive expression system in an adenoviral vector format. pAdenoX-CMV (included in the vector set) is a prelinearized, adenoviral vector that is ready for the insertion of your gene of interest via our In-Fusion HD PCR Cloning technology. Simply PCR-amplify your gene and combine it with pAdenoX-CMV in an In-Fusion HD Cloning reaction. In-Fusion HD Cloning is fast, simple, precise and efficient, making Adeno-X Adenoviral System 3 the most advanced commercially available adenoviral gene delivery tool.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

632267

Adeno-X™ Adenoviral System 3 (CMV, Green)

10 Rxns

$1253.00

License Statement

ID Number
28	This product is covered by the claims of U.S. Patent No. 7,575,860 and its foreign counterpart patent claims.
25	This product is covered by U.S. Patent No. 6,303,362.

Adeno-X Adenoviral System 3 (CMV, Green) provides the components necessary to generate a constitutive expression system in an adenoviral vector format. pAdenoX-ZsGreen1 (included in the vector set) is a prelinearized, adenoviral vector that is ready for the insertion of your gene of interest via our In-Fusion HD PCR Cloning technology. Simply PCR-amplify your gene and combine it with pAdenoX-ZsGreen1 in an In-Fusion HD Cloning reaction. In-Fusion HD Cloning is fast, simple, precise and efficient, making Adeno-X Adenoviral System 3 the most advanced commercially available adenoviral gene delivery tool. Adenoviral constructs created with this system will constitutively express your protein of interest and the fluorescent protein ZsGreen1 for visualization of infected mammalian cells.

Notice to purchaser

Components

632268

Adeno-X™ Adenoviral System 3 (CMV, Red)

10 Rxns

$1253.00

License Statement

ID Number
24	The RCFPs (including DsRedExpress, DsRedExpress2, and E2-Crimson) are covered by one or more of the following U.S. Patent Nos. 7,166,444; 7,157,565; 7,217,789; 7,338,784; 7,338,783; 6,969,597; 7,150,979; 7,442,522 and 8,012,682.
28	This product is covered by the claims of U.S. Patent No. 7,575,860 and its foreign counterpart patent claims.
72	Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the Adeno-X™ Adenoviral System 3 (CMV, Red) product License Agreement can be found by clicking here.
25	This product is covered by U.S. Patent No. 6,303,362.

Adeno-X Adenoviral System 3 (CMV, Red) provides the components necessary to generate a constitutive expression system in an adenoviral vector format. pAdenoX-DsRedExpress (included in the vector set) is a prelinearized, adenoviral vector that is ready for the insertion of your gene of interest via our In-Fusion HD PCR Cloning technology. Simply PCR-amplify your gene and combine it with pAdenoX-DsRedExpress in an In-Fusion HD Cloning reaction. In-Fusion HD Cloning is fast, simple, precise and efficient, making Adeno-X Adenoviral System 3 the most advanced commercially available adenoviral gene delivery tool. Adenoviral constructs created with this system will constitutively express your protein of interest and the fluorescent protein DsRed-Express for visualization of infected mammalian cells.

Notice to purchaser

Components

631180

Adeno-X™ Adenoviral System 3 (Tet-On® 3G Inducible)

10 Rxns

$1868.00

License Statement

ID Number
28	This product is covered by the claims of U.S. Patent No. 7,575,860 and its foreign counterpart patent claims.
42	Use of the Tetracycline controllable expression systems (the "Tet Technology") is covered by a series of patents including U.S. Patent # 7541446, # 8383364, # 9181556 , European patents EP # 1200607, # 1954811, #2352833 and corresponding patent claims outside these regions which are proprietary to TET Systems GmbH & Co. KG. Academic research institutions are granted an automatic license with the purchase of this product to use the Tet Technology only for internal, academic research purposes, which license specifically excludes the right to sell, or otherwise transfer, the Tet Technology or its component parts to third parties. Notwithstanding the above, academic and not-for profit research institutions whose research using the Tet Technology is sponsored by for profit organizations, which shall receive ownership to any data and results stemming from the sponsored research, shall need a commercial license agreement from TET Systems in order to use the Tet Technology. In accepting this license, all users acknowledge that the Tet Technology is experimental in nature. TET Systems GmbH & Co. KG makes no warranties, express or implied or of any kind, and hereby disclaims any warranties, representations, or guarantees of any kind as to the Tet Technology, patents, or products. All others are invited to request a license from TET Systems GmbH & Co. KG prior to purchasing these reagents or using them for any purpose. Takara Bio USA, Inc. is required by its licensing agreement to submit a report of all purchasers of the Tet-controllable expression system to TET Systems. For license information, please contact: GSF/CEO TET Systems GmbH & Co. KG, Im Neuenheimer Feld 582 69120 Heidelberg Germany Tel: +49 6221 5880400 Fax: +49 6221 5880404 email: info@tetsystems.com or use the electronic licensing request form via http://www.tetsystems.com/ip-licensing/licensing/for-profit-research
25	This product is covered by U.S. Patent No. 6,303,362.

Adeno-X Adenoviral System 3 (Tet-On 3G Inducible) provides the components necessary to generate a tightly controlled, highly sensitive, inducible expression system in an all-in-one adenoviral vector format. pAdenoX-Tet3G (included in the vector set) is a prelinearized, adenoviral vector that is ready for the insertion of your gene of interest with our In-Fusion HD PCR Cloning technology. Simply PCR-amplify your gene and combine it with pAdenoX-Tet3G in an In-Fusion HD Cloning reaction. In-Fusion HD Cloning is fast, simple, precise and efficient, making Adeno-X Adenoviral System 3 the most advanced commercially available adenoviral gene delivery tool.

Notice to purchaser

Components

Required Products

Cat. #	Product	Size	Price
631106	Tet System Approved FBS	500 mL	$865.00
Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. Documents Components Image Data Back Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems Fetal bovine serum (FBS) approved for use with tetracycline inducible expression systems. Only Takara Bio USA functionally tests FBS to be sure that no contaminating tetracycline derivatives interfere with induced expression levels. Back Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems Fetal bovine serum from Clontech is functionally tested to guarantee consistent results with tetracycline-inducible expression systems. Serum from other vendors that have not been functionally tested may contain tetracyclines that affect the maximum expression in Tet-Off systems, or generate background in Tet-On systems. Data here shows induction of luciferase expression from our CHO-AA8-Luc Tet-Off Control Cell Line using different sources of FBS.
631101	Tet System Approved FBS, US-Sourced	500 mL	$875.00
Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States. Documents Components Image Data Back Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems Fetal bovine serum (FBS) approved for use with tetracycline inducible expression systems. Only Takara Bio USA functionally tests FBS to be sure that no contaminating tetracycline derivatives interfere with induced expression levels. Back Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems Fetal bovine serum from Clontech is functionally tested to guarantee consistent results with tetracycline-inducible expression systems. Serum from other vendors that have not been functionally tested may contain tetracyclines that affect the maximum expression in Tet-Off systems, or generate background in Tet-On systems. Data here shows induction of luciferase expression from our CHO-AA8-Luc Tet-Off Control Cell Line using different sources of FBS.

632266

Adeno-X™ Adenoviral System 3 (Universal)

10 Rxns

$1092.00

License Statement

ID Number
28	This product is covered by the claims of U.S. Patent No. 7,575,860 and its foreign counterpart patent claims.
25	This product is covered by U.S. Patent No. 6,303,362.

Adeno-X Adenoviral System 3 (Universal) provides the components necessary to place an expression cassette of your choice in an adenoviral vector format. The system provides a perfect platform for the tissue-specific expression of your gene of interest, or for the efficient delivery of shRNA or microRNA. pAdenoX-PRLS (included in the vector set) is a prelinearized adenoviral vector that is ready for the insertion of your expression cassette via our In-Fusion HD PCR Cloning technology. Simply PCR-amplify the expression cassette and combine it with pAdenoX-PRLS in an In-Fusion HD Cloning reaction. In-Fusion HD Cloning is fast, simple, precise and efficient, making Adeno-X Adenoviral System 3 the most advanced commercially available adenoviral gene delivery tool.

Notice to purchaser

Components

632264

Adeno-X™ Adenoviral System 3 (Universal, Green)

10 Rxns

$1253.00

License Statement

ID Number
28	This product is covered by the claims of U.S. Patent No. 7,575,860 and its foreign counterpart patent claims.
25	This product is covered by U.S. Patent No. 6,303,362.

Adeno-X Adenoviral System 3 (Universal, Green) provides the components necessary to place an expression cassette of your choice in an adenoviral vector format. The system provides a perfect platform for the tissue-specific expression of your gene of interest, or for the efficient delivery of shRNA or microRNA. pAdenoX-PRLS-ZsGreen1 (included in the vector set) is a prelinearized adenoviral vector that is ready for the insertion of your expression cassette via our In-Fusion HD PCR Cloning technology. Simply PCR-amplify the expression cassette and combine it with pAdenoX-PRLS-ZsGreen1 in an In-Fusion HD Cloning reaction. In-Fusion HD Cloning is fast, simple, precise and efficient, making Adeno-X Adenoviral System 3 the most advanced commercially available adenoviral gene delivery tool. Adenoviral constructs created with this system will constitutively express the fluorescent protein ZsGreen1 in mammalian cells, allowing visualization of infected cells.

Notice to purchaser

Components

632265

Adeno-X™ Adenoviral System 3 (Universal, Red)

10 Rxns

$1253.00

License Statement

ID Number
24	The RCFPs (including DsRedExpress, DsRedExpress2, and E2-Crimson) are covered by one or more of the following U.S. Patent Nos. 7,166,444; 7,157,565; 7,217,789; 7,338,784; 7,338,783; 6,969,597; 7,150,979; 7,442,522 and 8,012,682.
28	This product is covered by the claims of U.S. Patent No. 7,575,860 and its foreign counterpart patent claims.
72	Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the Adeno-X™ Adenoviral System 3 (Universal, Red) product License Agreement can be found by clicking here.
25	This product is covered by U.S. Patent No. 6,303,362.

Adeno-X Adenoviral System 3 (Universal, Red) provides the components necessary to place an expression cassette of your choice in an adenoviral vector format. The system provides a perfect platform for the tissue-specific expression of your gene of interest, or for the efficient delivery of shRNA or microRNA. pAdenoX-PRLS-DsRedExpress (included in the vector set) is a prelinearized adenoviral vector that is ready for the insertion of your expression cassette via our In-Fusion HD PCR Cloning technology. Simply PCR-amplify the expression cassette and combine it with pAdenoX-PRLS-DsRedExpress in an In-Fusion HD Cloning reaction. In-Fusion HD Cloning is fast, simple, precise and efficient, making Adeno-X Adenoviral System 3 the most advanced commercially available adenoviral gene delivery tool. Adenoviral constructs created with this system will constitutively express the fluorescent protein DsRed-Express in mammalian cells, allowing visualization of infected cells.

Notice to purchaser

Components

*You must be logged in to a Business Account in order to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@takarabio.com.

Overview

Cloning into adenovirus is as simple and rapid as into any plasmid
Clone directly into the pAdenoX vector; no shuttle vector required
Obtain >90% cloning efficiency in just 2–3 days (other systems take 8 days minimum)
Highly flexible formats—use an existing expression cassette or create one without subcloning
Transduce dividing and non-dividing cells
Very high titer, high expression, broad host range
Tet-On 3G inducible adenoviral system is also available

More Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Tech notes describing the use of adenoviral vector systems from Takara Bio

Fastest & easiest adenoviral vector system ever

Until now, the main drawback of commercially supplied adenoviral vector systems has been the need to use complex cloning procedures to overcome the difficulties with cloning into large (~34 kb) plasmids. Procedures have included precloning into shuttle vectors and subcloning through multiple steps and multiple different strains of E. coli, all of which increase hands-on time and leave more room for error. At Takara Bio, our Adeno-X virologists thought "wouldn't it be great if you could clone directly into the adenoviral plasmid just like any plasmid?" They then harnessed the power of In-Fusion HD Cloning technology to make this happen.

How it works Tet-inducible version

Adenoviral selection guide

Browse our adenoviral selection guide to choose the best system for your needs. We offer both constitutive and Tet-inducible expression systems based on the Adeno-X adenoviral system 3, an advanced gene delivery system that quickly and efficiently produces adenoviral particles.

Selection guide Product finder tool

Takara Bio USA, Inc.
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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2019 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Adeno-X adenoviral system 3

There is no simpler adenoviral expression system

Tetracycline-inducible expression

Clone any expression cassette into the universal vectors

Notice to purchaser