Adeno-X adenoviral system 3
Adeno-X adenoviral system 3 is the most advanced commercially available adenoviral gene-delivery system—providing by far the simplest, fastest, and most efficient method for constructing recombinant adenoviral vectors. Seven formats are available, including the most advanced tetracycline-inducible expression system, constitutive expression systems with or without fluorescent reporters, and universal (promoterless) systems that allow you to clone and express any entire expression cassette of your choice.
Adeno-X adenoviral system 3 is the most advanced commercially available adenoviral gene-delivery system—providing the simplest, fastest, and most efficient method for constructing recombinant adenoviral vectors. Seven formats are available, including the most advanced tetracycline-inducible expression system, constitutive expression systems with a choice of fluorescent reporters, and promoterless systems that allow you to clone and express any entire expression cassette of your choice.
There is no simpler adenoviral expression system
The system relies on the ability of the In-Fusion HD enzyme to precisely recognize and fuse 15 bp of homology between two linear DNA molecules. To generate short regions of homology to the prelinearized pAdeno-X vector, simply add 15 bp of additional sequence to the primers you use to PCR amplify your gene of interest. Then, combine the DNA together with the In-Fusion HD enzyme in a 15-min reaction and transform Stellar Competent Cells. Cloning is always directional, and 90% of the clones contain the correct insert. The kit includes a control cloning fragment, primers for colony PCR verification of positive clones, and a NucleoBond Xtra kit for transfection-grade plasmid purification.
Tetracycline-inducible expression
When you clone your gene into pAdenoX-Tet3G, you are creating a system with the tightest and most sensitive control of gene expression. Tightly controlled, doxycycline-induced expression is as easy as constitutive expression since the Tet-On 3G transactivator protein and the PTRE3G controlled gene of interest are contained on the same adenoviral vector. Up to 3,000-fold induction can be achieved using this system.
Clone any expression cassette into the universal vectors
You are not limited to using a CMV expression system—we have created universal adenoviral systems with vectors that lack a promoter and polyA signal in the cloning site. Simply amplify an entire expression cassette (from promoter to polyA) from a pre-existing construct and clone using In-Fusion HD. Universal systems can be used for expression from alternative promoters that are more suitable to your target cell type such as EF1 alpha or tissue-specific promoters.
Overview
- Cloning into adenovirus is as simple and rapid as into any plasmid
- Clone directly into the pAdenoX vector; no shuttle vector required
- Obtain >90% cloning efficiency in just 2–3 days (other systems take 8 days minimum)
- Highly flexible formats—use an existing expression cassette or create one without subcloning
- Transduce dividing and non-dividing cells
- Very high titer, high expression, broad host range
- Tet-On 3G inducible adenoviral system is also available
More Information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Fastest & easiest adenoviral vector system ever
Until now, the main drawback of commercially supplied adenoviral vector systems has been the need to use complex cloning procedures to overcome the difficulties with cloning into large (~34 kb) plasmids. Procedures have included precloning into shuttle vectors and subcloning through multiple steps and multiple different strains of E. coli, all of which increase hands-on time and leave more room for error. At Takara Bio, our Adeno-X virologists thought "wouldn't it be great if you could clone directly into the adenoviral plasmid just like any plasmid?" They then harnessed the power of In-Fusion HD Cloning technology to make this happen.
How it works Tet-inducible version
Adenoviral selection guide
Browse our adenoviral selection guide to choose the best system for your needs. We offer both constitutive and Tet-inducible expression systems based on the Adeno-X adenoviral system 3, an advanced gene delivery system that quickly and efficiently produces adenoviral particles.
Selection guide Product finder toolTakara Bio USA, Inc.
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