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  • ‹ Back to RNA extraction and analysis for real-time qPCR
  • CellAmp whole transcriptome amplification kit
  • CellAmp direct RNA prep kit for real-time PCR
  • CellAmp direct RNA prep kit for qPCR or protein analysis
  • CellAmp Direct RNA Prep Kit for One Step RT-PCR (Real Time)
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Cells to cDNA: CellAmp whole transcriptome amplification kit for RT-PCR

The CellAmp Whole Transcriptome Amplification Kit (Real Time), Ver. 2 enables you to go from cells to cDNA that is ready for analysis by real-time PCR. When using conventional techniques for working with trace amounts of nucleic acids, loss of product during purification steps is a frequent problem. However, this cells-to-cDNA kit ensures good product yield because loss-inducing purification steps for RNA and cDNA are not required.

The CellAmp Whole Transcriptome Amplification Kit (Real Time), Ver. 2 enables you to go from cells to cDNA that is ready for analysis by real-time PCR. When using conventional techniques for working with trace amounts of nucleic acids, loss of product during purification steps is a frequent problem. However, this cells-to-cDNA kit ensures good product yield because loss-inducing purification steps for RNA and cDNA are not required.

The procedure begins with cell lysis, followed by a reverse transcription reaction with the dT adapter primer (RT dT Primer 2) to synthesize cDNA from mRNA. Next, terminal deoxynucleotidyl transferase (TdT) is used to add a dA tail to the synthesized cDNA. The resulting 3' dA tail containing cDNA product is used as direct template for PCR, resulting in fewer required amplification cycles.

In addition to moving from cells to cDNA, this product can be used to amplify cDNA from trace amounts of RNA.

The CellAmp Whole Transcriptome Amplification Kit (Real Time), Ver. 2 uses an additional exonuclease I treatment step, which helps suppress nonspecific primer-derived amplification and increases yields of low-expressed genes.

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Cat. # Product Size License Quantity Details
3734 CellAmp™ Whole Transcriptome Amplification Kit (Real Time), Ver. 2 100 Rxns USD $592.00

The CellAmp Whole Transcriptome Amplification Kit (Real Time) Ver. 2 allows direct amplification of cDNA from a small number of cells. The amplified cDNA can be used as a template for real-time PCR. When using conventional techniques for working with trace amounts of nucleic acids, loss of nucleic acid during purification steps is a frequent problem. However, this kit provides good yield because loss-inducing purification steps for RNA and cDNA are not required.

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Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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cDNA amplification from single cells

cDNA amplification from single cells
cDNA amplification from single cells
Reagent: TB Green Premix Ex Taq II (Tli RNase H Plus)
Target: Human B2M , RPLP2 , GAPDH
Instrument: Thermal Cycler Dice Real Time System

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3734: CellAmp Whole Transcriptome Amplification Kit (Real Time), Ver. 2

3734: CellAmp Whole Transcriptome Amplification Kit (Real Time), Ver. 2

Overview

  • Allow direct amplification of cDNA from a small number of cultured cells (<1,000) using real-time RT-PCR

  • Allows real-time PCR (qPCR) analysis of multiple genes from small samples

  • Expression analysis for low-expressed gene

More Information

Applications

  • Cells to cDNA with minimal purification

  • Real-time RT-PCR (RT-qPCR)

  • cDNA amplification from trace amounts of RNA

Choosing a PrimeScript kit for real-time RT-PCR

If you want to…

Use this…

Make cDNA using a master mix that includes RTase and primers

PrimeScript RT Master Mix (Perfect Real Time) (Cat. # RR036A and RR036B)

Make cDNA using a kit that provides RTase and primers as separate components

PrimeScript RT Reagent Kit (Perfect Real Time) (Cat. # RR037A and RR037B)

Avoid genomic DNA contamination during cDNA synthesis and RT-qPCR

PrimeScript RT Reagent Kit with gDNA Eraser (Perfect Real Time) (Cat. # RR047A and RR047B)

Perform 1-step real-time RT-PCR with TB Green

One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) (Cat. # RR086A and RR086B)

Prepare cDNA for RT-qPCR directly from fewer than 1,000 cells

CellAmp Whole Transcriptome Amplification Kit (Real Time), Ver. 2 (Cat. # 3734)

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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  • Single-cell cloning of edited hiPS cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
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  • Purified total RNA and mRNA
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  • PCR master mixes
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