PrimeScript RT Master Mix (Perfect Real Time): Generate cDNA for real-time PCR
To minimize sample-handling time, look no further than this real-time RT-PCR kit. PrimeScript RT Master Mix (Perfect Real Time) is designed for fast, efficient reverse transcription prior to real-time RT-PCR (qPCR) with a streamlined workflow. cDNA synthesis reactions are easy to assemble—just add RNA template and water. The real-time RT-PCR kit contains a 5X master mix reagent that includes all reagents required for cDNA synthesis (PrimeScript RTase, RNase inhibitor, random 6mers, oligo dT primer, dNTPs, and reaction buffer). Because this real-time RT-PCR kit is powered by PrimeScript Reverse Transcriptase, it enables full-length cDNA synthesis for real-time RT-PCR (qPCR) in just 15 minutes. This kit is designed for two-step real-time RT-PCR. Use it upstream of a TB Green- or probe-based qPCR premix from Takara Bio, such as TB Green Premix Ex Taq II (Tli RNase H), TB Green Premix Ex Taq (Tli RNase H), or Premix Ex Taq (Probe qPCR).
To minimize sample-handling time, look no further than this real-time RT-PCR kit. PrimeScript RT Master Mix (Perfect Real Time) is designed for fast, efficient reverse transcription prior to real-time RT-PCR (qPCR) with a streamlined workflow. cDNA synthesis reactions are easy to assemble—just add RNA template and water. The real-time RT-PCR kit contains a 5X master mix reagent that includes all reagents required for cDNA synthesis (PrimeScript RTase, RNase inhibitor, random 6mers, oligo dT primer, dNTPs, and reaction buffer). Because this real-time RT-PCR kit is powered by PrimeScript Reverse Transcriptase, it enables full-length cDNA synthesis for real-time RT-PCR (qPCR) in just 15 minutes. This kit is designed for two-step real-time RT-PCR. Use it upstream of a TB Green- or probe-based qPCR premix from Takara Bio, such as TB Green Premix Ex Taq II (Tli RNase H), TB Green Premix Ex Taq (Tli RNase H), or Premix Ex Taq (Probe qPCR).
This real-time RT-PCR kit includes PrimeScript reverse transcriptase, which has exceptionally strong strand-displacement activity for optimal efficiency during cDNA synthesis. The PrimeScript recombinant RTase enzyme is robust, versatile, and well-suited for applications requiring full-length cDNA synthesis. PrimeScript RTase enables reverse transcription of virtually any RNA template, including templates that are GC-rich and/or have high levels of secondary structure, making this real-time RT-PCR kit ideal for both routine applications and challenging reverse transcriptase reactions alike. PrimeScript RTase is a modified, recombinant MMLV (Moloney murine leukemia virus) enzyme and is verified to be RNase H-minus. PrimeScript reverse transcriptase is engineered to allow full-length cDNA synthesis reactions at lower temperatures (e.g., 42°C), decreasing the risk of RNA degradation that occurs during conventional reactions performed at higher temperatures.
Overview
-
Reverse transcription in just 15 minutes
-
Master mix format reduces pipetting steps and the possibility of contamination
-
Works efficiently with GC-rich and/or higher-structured RNA templates
-
Synthesizes full-length cDNA with high accuracy and at high yield prior to qPCR analysis
More Information
Applications
-
First-strand cDNA synthesis for real-time RT-PCR (qPCR)
-
Two-step real-time RT-PCR
Choose the right PrimeScript Kit for Real-Time RT-PCR:
| If you want to.... | Use this.... |
| Make cDNA using a master mix that includes RTase and primers | PrimeScript RT Master Mix (Perfect Real Time) (Cat. # RR036A and RR036B) |
| Make cDNA using a kit that provides RTase and primers as separate components | PrimeScript RT Reagent Kit (Perfect Real Time) (Cat. # RR037A and RR037B) |
| Avoid genomic DNA contamination during cDNA synthesis and RT-qPCR | PrimeScript RT Reagent Kit with gDNA Eraser (Perfect Real Time) (Cat. # RR047A and RR047B) |
| Perform 1-step real-time RT-PCR with TB Green | One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) (Cat. # RR086A and RR086B) |
| Prepare cDNA for RT-qPCR directly from fewer than 1,000 cells | CellAmp Whole Transcriptome Amplification Kit (Real Time), Ver. 2 (Cat. # 3734) |
Product citations
Komori, H. et al. α(1)-Acid glycoprotein up-regulates CD163 via TLR4/CD14 protein pathway: possible protection against hemolysis-induced oxidative stress. J. Biol. Chem. 287, 30688–700 (2012).
Liu, H. et al. Metabolism and Pharmacokinetics of Mangiferin in Conventional Rats, Pseudo-Germ-Free Rats, and Streptozotocin-Induced Diabetic Rats. Drug Metab. Dispos. 40, (2012).
Shimura, Y., Shiraiwa, Y. & Suzuki, I. Characterization of the subdomains in the N-terminal region of histidine kinase Hik33 in the cyanobacterium Synechocystis sp. PCC 6803. Plant Cell Physiol. 53, 1255–66 (2012).
Tani, H. et al. Genome-wide determination of RNA stability reveals hundreds of short-lived noncoding transcripts in mammals. Genome Res. 22, 947–56 (2012).
Yasuhara, K. et al. Absence of heat shock transcription factor 1 retards the regrowth of atrophied soleus muscle in mice. J. Appl. Physiol. 111, 1142–9 (2011).
Zhan, Y. et al. A novel taspine derivative, HMQ1611, inhibits breast cancer cell growth via estrogen receptor α and EGF receptor signaling pathways. Cancer Prev. Res. (Phila). 5, 864–73 (2012).
Zhang, X. et al. Rare allele of OsPPKL1 associated with grain length causes extra-large grain and a significant yield increase in rice. Proc. Natl. Acad. Sci. U. S. A. 109, 21534–9 (2012).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2019 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.
