PrimeScript™ RT Master Mix (Perfect Real Time)

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Cat. # Product Size Price License Quantity Details
9160 EASY Dilution (for Real Time PCR) 8 x 1 mL USD $151.00

This product is used to dilute template DNA or RNA when preparing serial dilutions of the standard for establishing the standard curve in real time PCR.

RR390A Premix Ex Taq™ (Probe qPCR) 200 Rxns USD $270.00

Premix Ex Taq (Probe qPCR) is a 2X premix for real-time PCR (qPCR) detection with probe-based qPCR or 5' nuclease assays. The 2X premix includes Takara Ex Taq HS, which contains a hot start PCR enzyme with an anti-Taq antibody, and a buffer optimized for real-time PCR. The resulting premix allows excellent suppression of non-specific amplification, high amplification efficiency, and high detection sensitivity in real-time PCR analyses. In addition, Tli RNaseH, a heatresistant RNaseH, is included to minimize PCR inhibition from residual mRNA in reactions using cDNA templates. This product is excellent for high-speed PCR and allows accurate target quantification and detection over a broad dynamic range, making it possible to conduct highly reproducible and reliable real-time PCR analyses.

RR390B Premix Ex Taq™ (Probe qPCR) 400 Rxns USD $536.00

Premix Ex Taq (Probe qPCR) is a 2X premix for real-time PCR (qPCR) detection with probe-based qPCR or 5' nuclease assays. The 2X premix includes Takara Ex Taq HS, which contains a hot start PCR enzyme with an anti-Taq antibody, and a buffer optimized for real-time PCR. The resulting premix allows excellent suppression of non-specific amplification, high amplification efficiency, and high detection sensitivity in real-time PCR analyses. In addition, Tli RNaseH, a heatresistant RNaseH, is included to minimize PCR inhibition from residual mRNA in reactions using cDNA templates. This product is excellent for high-speed PCR and allows accurate target quantification and detection over a broad dynamic range, making it possible to conduct highly reproducible and reliable real-time PCR analyses. Cat. # RR390B contains 2 of Cat. # RR390A. Please refer to Cat. # RR390A for complete product documentation and resources.

RR420A TB Green® Premix Ex Taq™ (Tli RNase H Plus) 200 Rxns USD $337.00

TB Green Premix Ex Taq (Tli RNase H Plus) provides reduced PCR inhibition caused by the presence of double-stranded RNA/cDNA hybrids remaining after cDNA synthesis. This inhibition is common when using low or RNase H minus RTs, and with GC-rich templates and/or genes with poor expression. The presence of dsRNA/cDNA hybrids is a frequent cause of poor qPCR amplification and/or reaction failure. TB Green Premix Ex Taq (Tli RNase H Plus) prevents this potential problem at no additional cost and without requiring a separate RNase H digestion step prior to qPCR.

RR420B TB Green® Premix Ex Taq™ (Tli RNase H Plus) 400 Rxns USD $635.00

TB Green Premix Ex Taq (Tli RNase H Plus) provides reduced PCR inhibition caused by the presence of double-stranded RNA/cDNA hybrids remaining after cDNA synthesis. This inhibition is common when using low or RNase H minus RTs, and with GC-rich templates and/or genes with poor expression. The presence of dsRNA/cDNA hybrids is a frequent cause of poor qPCR amplification and/or reaction failure. TB Green Premix Ex Taq (Tli RNase H Plus) prevents this potential problem at no additional cost and without requiring a separate RNase H digestion step prior to qPCR. Cat. # RR420B contains 2 of Cat. # RR420A. Please refer to Cat. # RR420A for complete product documentation and resources.

RR820A TB Green® Premix Ex Taq™ II (Tli RNase H Plus) 200 Rxns USD $337.00

TB Green Premix Ex Taq II (Tli RNase H Plus) is a reagent specifically designed for intercalator-based real time PCR using TB Green. It is supplied at a 2X concentration premixed with TB Green at a concentration appropriate for real time monitoring, making it easy to prepare reaction mixtures. The 2X premixed reagent also contains Tli RNase H, a heat-resistant RNase H, which minimizes PCR inhibition due to residual mRNA when using cDNA as template. This product, with a modified buffer composition, offers a higher reaction specificity than that of TB Green Premix Ex Taq (Tli RNaseH Plus) (Cat.# RR420A). With inhibition of non-specific amplifications, which interfere with quantitative determination, accurate assays over a wide range are possible. A combination of this buffer and Takara Ex Taq HS, a hot start PCR enzyme that uses an anti-Taq antibody, allows highly reproducible and reliable real time PCR analyses.

RR820B TB Green® Premix Ex Taq™ II (Tli RNase H Plus) 400 Rxns USD $635.00

TB Green Premix Ex Taq II (Tli RNase H Plus) is a reagent specifically designed for intercalator-based real time PCR using TB Green. It is supplied at a 2X concentration premixed with TB Green at a concentration appropriate for real time monitoring, making it easy to prepare reaction mixtures. The 2X premixed reagent also contains Tli RNaseH, a heat-resistant RNase H, which minimizes PCR inhibition due to residual mRNA when using cDNA as template. This product, with a modified buffer composition, offers a higher reaction specificity than that by TB Green Premix Ex Taq (Tli RNaseH Plus) (Cat.# RR420A). With inhibition of non-specific amplifications, which interfere with quantitative determination, accurate assays over a wide range are possible. A combination of this buffer and Takara Ex Taq HS, a hot start PCR enzyme that uses an anti-Taq antibody, allows highly reproducible and reliable real time PCR analyses.