TB Green Advantage qPCR premixes

TB Green Advantage qPCR Premix is a convenient, ready-to-use 2X-concentrated master mix for real-time PCR that contains full-length Taq polymerase with hot-start antibody and TB Green dye. It is specially designed for real-time PCR employing the dye intercalator method. This qPCR master mix combines the high performance of our enzyme for hot-start PCR utilizing Taq antibody and a buffer developed to provide superior specificity, increased amplification efficiency, and excellent performance in high-speed, real-time PCR. Use of the TB Green Advantage qPCR Premix enables you to carry out successful real-time PCR with high sensitivity, broad dynamic range, and accurate quantification.

TB Green Advantage qPCR Premix is supplied separately with ROX Reference Dye LSR and ROX Reference Dye LMP, which allow you to normalize the fluorescence signal between reactions on instruments that are equipped with this option.

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Cat. #	Product	Size	Price	License	Quantity	Details
639676	TB Green® Advantage® qPCR Premix	200 Rxns	$169.00
TB Green Advantage qPCR Premix is a convenient 2X master mix containing TB Green dye, full-length Taq DNA Polymerase, hot-start antibody, dNTPs, and buffer (including Mg²⁺). The mix comes with two separate tubes of ROX passive reference dye: ROX Reference Dye LSR contains the optimal concentration of ROX for instruments whose excitation source is a 488 nm laser; and ROX Reference Dye LMP contains the optimal concentration of ROX for instruments whose excitation source is either a lamp or an LED. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Reaction specificity—performance of Takara Bio TB Green Advantage qPCR Premix vs. Competitor R's qPCR mix using a Roche LightCycler. Reaction specificity—performance of Takara Bio TB Green Advantage qPCR Premix vs. Competitor R's SYBR mix using a Roche LightCycler. The results for the Takara Bio reagent are shown in Panels A and C, and those for Competitor R's reagent are shown in Panels B and D. The cycling conditions for our reagent consisted of 1 cycle at 95°C for 10 min, followed by 45 cycles at 95°C for 5 sec and 60°C for 20 sec. For Competitor R's reagent, the cycling conditions consisted of 1 cycle at 95°C for 10 min, followed by 45 cycles at 94°C for 10 sec, 55°C for 5 sec, and 72°C for 10 sec. Back Amplification efficiency—performance of TB Green Advantage qPCR Premix vs. Competitor A's qPCR mix using an ABI PRISM 7000 Sequence Detection System. Amplification efficiency—performance of TB Green Advantage qPCR Premix vs. Competitor A's SYBR mix using an ABI PRISM 7000 Sequence Detection System. The results for the TB Green reagent are shown in Panels A and C, and those for Competitor A's reagent are shown in Panels B and D. The cycling conditions for the TB Green reagent consisted of 1 cycle at 95°C for 10 sec, followed by 40 cycles at 95°C for 5 sec and 60°C for 31 sec. For Competitor A's reagent, the cycling conditions consisted of 1 cycle at 95°C for 10 min, followed by 45 cycles at 95°C for 15 sec and 60°C for 1 min. Back Reaction specificity—Takara Bio's TB Green Advantage qPCR Premix vs. Competitor I's qPCR mix using a Cepheid Smart Cycler. Reaction specificity—performance of Takara Bio's TB Green Advantage qPCR Premix vs. Competitor I's qPCR mix using a Cepheid Smart Cycler. The results for the Takara Bio reagent are shown in Panels A and C, and those for Competitor I's reagent are shown in Panels B and D. The cycling conditions for the TB Green reagent consisted of 1 cycle at 95°C for 2 min, followed by 45 cycles at 95°C for 5 sec and 60°C for 20 sec. For Competitor I's reagent, the cycling conditions consisted of 1 cycle at 95°C for 2 min, followed by 45 cycles at 95°C for 15 sec and 60°C for 30 sec.

Overview

Provided in a convenient premixed format containing TB Green intercalating dye
Optimized for use with the laser-, lamp-, or LED-based real-time instrument of your choice
Compatible with all real-time PCR instruments

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Applications

Real-time PCR/qPCR
Gene expression analysis
Genotyping
CpG island amplification

Recent studies have shown that the TB Green Advantage qPCR Premix is superior to corresponding reagents offered by three leading competitors in the qPCR arena, in terms of reaction specificity, amplification efficiency, and sensitivity, as well as speed.

More specific than Competitor R

Competitor R's real-time PCR enzyme showed poor reaction specificity when compared to the TB Green Advantage qPCR Premix. This was evidenced by multiple peaks in the melting curve for Competitor R's enzyme, particularly when low-copy-number templates were amplified.

More efficient than Competitor A

Competitor A's SYBR qPCR master mix showed a lower amplification efficiency than that of the TB Green Advantage qPCR Premix. This was indicated by Ct values that were shifted to the right.

More specific than Competitor I

The TB Green Advantage qPCR Premix demonstrated superior reaction specificity when compared to Competitor I's SYBR qPCR master mix. This was indicated by tighter peaks in the melting curve for the Takara Bio enzyme.

Twice as fast as Competitor A

Excellent results were obtained in about half the time (50 min versus 90 min) when using the TB Green Advantage qPCR Premix as opposed to Competitor A's master mix for probe-based qPCR assays (data not shown). These experiments were carried out with the Applied Biosystems 7500 Real-Time PCR System.

Clearly, the Takara Bio TB Green Advantage qPCR Premix outperforms much of the competition in terms of specificity (melting curves indicate the presence of a single product), efficiency, and sensitivity (lower Ct values in the amplification plots). Moreover, when using our TB Green Advantage qPCR Premix, qPCR analysis can be accomplished in nearly half of the time required for other qPCR chemistries.

Compatible cyclers

SmartCycler, LightCycler, ABI PRISM 7000/7700/7900 HT, Applied Biosystems StepOne and StepOnePlus, 7300/7500, iCycler, Opticon, Stratagene MX 3000P and MX3005P, and QIAGEN Rotor-Gene.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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