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  • ‹ Back to DNA-seq
  • PicoPLEX Gold for single cells
  • DNA-seq for FFPE and cell-free DNA
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Technical notes Outperform MDA for SNV detection
Accurate detection of SNVs and CNVs from five-cell inputs in a single, low-pass sequencing run A single NGS assay for SNVs and CNVs
Home › Products › Next-generation sequencing › DNA-seq › PicoPLEX Gold for single cells

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Technical notes Outperform MDA for SNV detection
Accurate detection of SNVs and CNVs from five-cell inputs in a single, low-pass sequencing run A single NGS assay for SNVs and CNVs

Single-cell library prep for SNV and CNV detection

PicoPLEX Gold DNA-Seq

The PicoPLEX Gold Single Cell DNA-Seq Kit (PicoPLEX Gold) is designed to generate high-quality DNA libraries from single cells for sequencing on Illumina platforms. The kit is based on our patented PicoPLEX technology and is optimized for the reproducible detection of copy number variants (CNVs), single nucleotide variants (SNVs), indels, and small structural variants. The PicoPLEX Gold kit outperforms the leading Multiple Displacement Amplification (MDA) technology for SNV detection.

The PicoPLEX Gold Single Cell DNA-Seq Kit has been reconfigured from the original versions of PicoPLEX kits and does not include indexes. Compatible index kits are described below and can be purchased here.

The PicoPLEX Gold Single Cell DNA-Seq Kit (PicoPLEX Gold) is designed to generate high-quality DNA libraries from single cells for sequencing on Illumina platforms. The kit is based on our patented PicoPLEX technology and is optimized for the reproducible detection of copy number variants (CNVs), single nucleotide variants (SNVs), indels, and small structural variants. The PicoPLEX Gold kit outperforms the leading Multiple Displacement Amplification (MDA) technology for SNV detection.

The PicoPLEX Gold Single Cell DNA-Seq Kit has been reconfigured from the original versions of PicoPLEX kits and does not include indexes. Compatible index kits are described below and can be purchased here.

PicoPLEX WGA (PicoPLEX) quasi-random priming technology is the international gold standard for whole genome amplification (WGA) for subsequent detection of CNVs in fixed or unfixed single cells. Initially, PicoPLEX chemistry was created for the reproducible detection of aneuploidies and CNVs. To address the need for accurate detection of SNVs, we further optimized the PicoPLEX WGA chemistry using enzymes, primers, and protocols that improve sequencing coverage, uniformity, and accuracy while increasing the resolution for CNVs, SNVs, indels, and other small structural variants while retaining reproducibility.

The enhanced chemistry named PicoPLEX Gold is a single-cell library prep kit with a simple, four-step protocol to convert fixed or unfixed single cells into NGS libraries in under three hours with minimum hands-on time.

DNA HT Dual Index kits

DNA HT Dual Index kits are designed for use with ThruPLEX and PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the Illumina Nextera® XT v2 index sequences and offer a total of 384 dual indexes for multiplexing of up to 384 samples. The indexed PCR primers are supplied pre-dispensed in four different barcoded index plates, each of which contains one-fourth of the possible dual index combinations (Cat. # R400660–R400663); or in a set of 24 individual tubes, each containing a different dual index combination (Cat. # R400664). Each dual index tube (24N) contains sufficient volume for up to two uses. Each well of a dual index plate (96N Sets A–D) contains sufficient volume for a single use.

DNA Unique Dual Index kits

DNA Unique Dual Index kits are designed for use with ThruPLEX and PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the "IDT for Illumina UD" index sequences and offer a total of 96 dual indexes for multiplexing of up to 96 samples. The indexed PCR primers are supplied pre-dispensed in four different sets of 24 individual tubes, each of which contains one-fourth of the possible dual index combinations (Cat. Nos. R400665–R400668). Each dual index tube (24U Sets A–D) contains sufficient volume for up to two uses.

DNA Single Index kits

DNA Single Index kits are designed for use with ThruPLEX and PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the "TruSeq LT set A" index sequences and offer a total of 12 single indexes for multiplexing of up to 12 samples. The indexed PCR primers are supplied pre-dispensed in four different sets of 12 individual tubes, each containing a different index sequence (Cat. Nos. R400695 and R400697). Each single index tube (12S Sets A–B) contains sufficient volume for up to eight uses.

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Cat. # Product Size License Quantity Details
R300669 PicoPLEX® Gold Single Cell DNA-seq Kit 24 Rxns USD $1080.00

License Statement

ID Number  
325 Patent pending. For further information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
M54 This product is covered by the claims of U.S. Patent Nos. 7,704,713 and its foreign counterparts. 
328 This product is protected by U.S. Patents 8,206,913 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

The PicoPLEX Gold Single Cell DNA-Seq Kit is designed to generate high-quality DNA libraries from single cells for sequencing on Illumina platforms. The kit is based on innovative PicoPLEX technology and yields libraries with superior reproducibility from 1–5 mammalian cells or genome equivalents of gDNA as input, providing reliable results from challenging samples such as formalin-fixed single cells. Single index, dual index, and unique dual index kits are available and must be purchased separately. This product contains reagents for 24 reactions.

The kit features a streamlined workflow consisting of four steps—from input to amplified NGS libraries—that can be completed in less than four hours. The kit uses high-fidelity enzymes and optimized primers and is suitable for accurate and reproducible profiling of copy number variants (CNVs), single-nucleotide variants (SNVs), indels, and other small structural variants. The kit is compatible with single cells and purified DNA from humans, animals, and bacteria, as well as single flow-sorted chromosomes.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

SMARTer PicoPLEX Gold Single Cell DNA-Seq technology: a four-step reaction that starts with a single cell

SMARTer PicoPLEX Gold Single Cell DNA-Seq technology: a four-step reaction that starts with a single cell

SMARTer PicoPLEX Gold Single Cell DNA-Seq technology: a four-step reaction that starts with a single cell. Cellular gDNA extracted in Step 1 is used as template for multiple cycles of quasi-random priming and linear amplification followed by exponential library amplification.

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SMARTer PicoPLEX Gold Single Cell DNA-Seq Kit workflow

SMARTer PicoPLEX Gold Single Cell DNA-Seq Kit workflow

SMARTer PicoPLEX Gold Single Cell DNA-Seq Kit workflow. The four-step SMARTer PicoPLEX Gold Single Cell DNA-Seq workflow takes place in two tubes or plates and is completed in less than 3 hours with just 30 minutes of hands-on time.

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R300669: PicoPLEX Gold Single Cell DNA-seq Kit

R300669: PicoPLEX Gold Single Cell DNA-seq Kit
R300670 PicoPLEX® Gold Single Cell DNA-seq Kit 96 Rxns USD $3779.00

License Statement

ID Number  
M54 This product is covered by the claims of U.S. Patent Nos. 7,704,713 and its foreign counterparts. 
325 Patent pending. For further information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
328 This product is protected by U.S. Patents 8,206,913 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

The PicoPLEX Gold Single Cell DNA-Seq Kit is designed to generate high-quality DNA libraries from single cells for sequencing on Illumina platforms. The kit is based on innovative PicoPLEX technology and yields libraries with superior reproducibility from 1–5 mammalian cells or genome equivalents of gDNA as input, providing reliable results from challenging samples such as formalin-fixed single cells. Single index, dual index, and unique dual index kits are available and must be purchased separately. This product contains reagents for 96 reactions.

The kit features a streamlined workflow consisting of four steps—from input to amplified NGS libraries—that can be completed in less than four hours. The kit uses high-fidelity enzymes and optimized primers and is suitable for accurate and reproducible profiling of copy number variants (CNVs), single-nucleotide variants (SNVs), indels, and other small structural variants. The kit is compatible with single cells and purified DNA from humans, animals, and bacteria, as well as single flow-sorted chromosomes.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

SMARTer PicoPLEX Gold Single Cell DNA-Seq technology: a four-step reaction that starts with a single cell

SMARTer PicoPLEX Gold Single Cell DNA-Seq technology: a four-step reaction that starts with a single cell

SMARTer PicoPLEX Gold Single Cell DNA-Seq technology: a four-step reaction that starts with a single cell. Cellular gDNA extracted in Step 1 is used as template for multiple cycles of quasi-random priming and linear amplification followed by exponential library amplification.

Back

SMARTer PicoPLEX Gold Single Cell DNA-Seq Kit workflow

SMARTer PicoPLEX Gold Single Cell DNA-Seq Kit workflow

SMARTer PicoPLEX Gold Single Cell DNA-Seq Kit workflow. The four-step SMARTer PicoPLEX Gold Single Cell DNA-Seq workflow takes place in two tubes or plates and is completed in less than 3 hours with just 30 minutes of hands-on time.

Back

R300670: PicoPLEX Gold Single Cell DNA-seq Kit

R300670: PicoPLEX Gold Single Cell DNA-seq Kit
R300698 PicoPLEX® Gold Single Cell DNA-Seq Kit 384 Rxns USD $11225.00

License Statement

ID Number  
325 Patent pending. For further information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
M54 This product is covered by the claims of U.S. Patent Nos. 7,704,713 and its foreign counterparts. 
328 This product is protected by U.S. Patents 8,206,913 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

The PicoPLEX Gold Single Cell DNA-Seq Kit is designed to generate high-quality DNA libraries from single cells for sequencing on Illumina platforms. The kit is based on innovative PicoPLEX technology and yields libraries with superior reproducibility from 1–5 mammalian cells or genome equivalents of gDNA as input, providing reliable results from challenging samples such as formalin-fixed single cells. Single index, dual index, and unique dual index kits are available and must be purchased separately. This product is composed of four 96-reaction kits (Cat. # R300670), 384 reactions total, of PicoPLEX Gold Single Cell DNA-Seq reagents.

The kit features a streamlined workflow consisting of four steps—from input to amplified NGS libraries—that can be completed in less than four hours. The kit uses high-fidelity enzymes and optimized primers and is suitable for accurate and reproducible profiling of copy number variants (CNVs), single-nucleotide variants (SNVs), indels, and other small structural variants. The kit is compatible with single cells and purified DNA from humans, animals, and bacteria, as well as single flow-sorted chromosomes.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

SMARTer PicoPLEX Gold Single Cell DNA-Seq technology: a four-step reaction that starts with a single cell

SMARTer PicoPLEX Gold Single Cell DNA-Seq technology: a four-step reaction that starts with a single cell

SMARTer PicoPLEX Gold Single Cell DNA-Seq technology: a four-step reaction that starts with a single cell. Cellular gDNA extracted in Step 1 is used as template for multiple cycles of quasi-random priming and linear amplification followed by exponential library amplification.

Back

SMARTer PicoPLEX Gold Single Cell DNA-Seq Kit workflow

SMARTer PicoPLEX Gold Single Cell DNA-Seq Kit workflow

SMARTer PicoPLEX Gold Single Cell DNA-Seq Kit workflow. The four-step SMARTer PicoPLEX Gold Single Cell DNA-Seq workflow takes place in two tubes or plates and is completed in less than 3 hours with just 30 minutes of hands-on time.

Back

R300698: SMARTer PicoPLEX Gold Single Cell DNA-seq Kit

R300698: SMARTer PicoPLEX Gold Single Cell DNA-seq Kit

DNA index kits DNA index kits (required)

Overview

  • Highly reproducible and accurate CNV, SNV, and aneuploidy detection
  • Outperforms the leading MDA technology for SNV and CNV detection
  • Streamlined three-hour workflow with less than 30 minutes of hands-on time
  • Highly uniform coverage with low allele drop-in and drop-out rate

More Information

Applications

Library preparation for the following cell types:

  • Cancer research: circulating tumor cells, cells, or DNA from laser capture microdissection (LCM), and cells from fine needle aspirate (FNA)
  • Reproductive health research: embryonic cells and circulating fetal cells
  • Developmental biology: embryonic cells, stem cells, and rare cells
  • Metagenomics and microbiology: microbial cells, microbiome and environmental samples

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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