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  • Xfect reagent
  • Xfect for mES cells
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Transfection reagents

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    • Xfect for mES cells
    • Calcium phosphate transfection
    • Fluorescent transfection controls
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Xfect mouse embryonic stem cell transfection reagent

Xfect mouse embryonic stem cell transfection reagent

Xfect mESC Transfection Reagent provides the highest transfection efficiency in multiple mES cell lines, including ES-E14TG2a and ES-D3 mES cell lines. This reagent is specifically recommended for mouse ES cells; for transfecting other cell types, including adult stem cells, we recommend Xfect Transfection Reagent.

Cat. # Product Size Price License Quantity Details
631320 Xfect™ mESC Transfection Reagent 100 Rxns USD $343.00

License Statement

ID Number  
63 Use of this product is covered by one or more of the following U.S. Patent Nos. and corresponding patent claims outside the U.S.: 8,562,966, 8,557,231. This product is intended for research purposes only. It may not be used for (i) any human or veterinary use, including without limitation therapeutic and prophylactic use, (ii) any clinical use, including without limitation diagnostic use, (iii) screening of chemical and/or biological compounds for the identification of pharmaceutically active agents (including but not limited to screening of small molecules), target validation, preclinical testing services, or drug development. Any use of this product for any of the above mentioned purposes requires a license from the Massachusetts Institute of Technology.

Xfect mESC is a transfection reagent that creates biodegradable nanoparticles that permit superior transfection efficiency of plasmid DNA into mouse embryonic stem cells. Transfections can be carried out entirely in the presence of serum.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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631320: Xfect mESC Transfection Reagent

631320: Xfect mESC Transfection Reagent

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Effective transfection of embryonic stem cells with Xfect mESC Transfection Reagent

Effective transfection of embryonic stem cells with Xfect mESC Transfection Reagent

Effective transfection of embryonic stem cells with Xfect mESC Transfection Reagent. ES-E14TG2a mES cells were transfected with 5 µg of DsRed-Express2 plasmid using 250 µg of Xfect mESC Transfection Reagent. 48 hr posttransfection, transfection efficiency was assessed via flow cytometry (left panel) as well as phase and fluorescent microscopy (middle and right panels, respectively). As quantified by flow cytometric analysis, 93.9% of the cells transfected with Xfect mESC Transfection Reagent were positive for DsRed-Express2 expression (left panel), with an MFI of 716.9 (data not shown).

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Xfect mESC Transfection Reagent provides superior transfection efficiency in mES cells

Xfect mESC Transfection Reagent provides superior transfection efficiency in mES cells

Xfect mESC Transfection Reagent provides superior transfection efficiency in mES cells. Xfect mESC Transfection Reagent and three competitor reagents were used according to their respective protocols to transfect mES cell lines using plasmid DNA encoding AcGFP1, in a 6-well format. 48 hr posttransfection, AcGFP1 expression was assessed by flow cytometry in order to determine transfection efficiency.

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Xfect mESC Transfection Reagent exhibits far higher transfection efficiency than leading competitor reagents

Xfect mESC Transfection Reagent exhibits far higher transfection efficiency than leading competitor reagents
Xfect mESC Transfection Reagent exhibits far higher transfection efficiency than leading competitor reagents. ES-E14TG2a mES cells were transfected in a 6-well plate format with a plasmid expressing AcGFP1 using Xfect mESC Transfection Reagent (Panel A), Product L (Panel B), Product LX (Panel C), or Product F (Panel D) according to each manufacturer’s recommended protocol. 48 hr posttransfection, the cells were imaged using white light (top) and by fluorescence microscopy using a Zeiss Axioskop microscope equipped with a GFP filter (bottom).

Overview

  • mES cell-specific transfection reagent (for other cell types try Xfect Transfection Reagent)
  • >90% transfection efficiency
  • Polymer forms biodegradable nanoparicles with plasmid DNA
  • Low cytotoxicity—obtain biologically relevant data, with no undesired effects
  • Simple, serum-compatible protocol

More Information

Applications

  • Transfection and gene expression in mES cells.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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  • Protein research
  • Antibodies and ELISA
  • New products
  • Special offers
  • COVID-19 research
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Next-generation sequencing
  • Spatial omics
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Bioinformatics tools
  • Immune profiling
  • Real-time PCR
  • Great value master mixes
  • Signature enzymes
  • High-throughput real-time PCR solutions
  • Detection assays
  • References, standards, and buffers
  • Stem cell research
  • Media, differentiation kits, and matrices
  • Stem cells and stem cell-derived cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Automated platforms
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
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  • Viral transduction
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  • Transfection reagents
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