DNA molecular weight markers

Our selection of DNA ladders and DNA molecular weight markers—with fragment sizes ranging from 20 bp to 2.5 kb—are suitable as size standards for various applications, such as nucleic acid size and quantity determination during agarose gel electrophoresis. In addition, we also offer several standard lambda digests and phiX174 digests in low, mid, and high size ranges. These ladders are premixed with loading dye and glycerol for direct agarose gel loading.

Three wide-range DNA ladders, consisting of quantitated DNA fragments ranging in size from 100–2,000 bp, 100–5,000 bp, and 50–10,000 bp, allow the determination of DNA size and quantity over a broad molecular range.

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Cat. #	Product	Size	Price
3428A	Wide-Range DNA Ladder (100-5,000 bp)	100 Rxns	USD $183.00
This marker consists of nine double-stranded DNA fragments (100, 250, 500, 750, 1,000, 1,500, 2,000, 3,000, and 5,000 bp). The 1,000 bp fragment is brighter than the other fragments and serves as a visible reference indicator; all other fragments appear with equal intensity on the gel. Since this product is premixed with loading dyes and glycerol, it is possible to use this product directly in agarose gel electrophoresis. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Migration patterns for Wide Range DNA Ladders: Cat.# 3415A (50-10,000 bp), Cat.# 3427A (100-2,000 bp), and Cat.# 3428A (100-5,000 bp) Migration patterns for Wide Range DNA Ladders: Cat.# 3415A (50-10,000 bp), Cat.# 3427A (100-2,000 bp), and Cat.# 3428A (100-5,000 bp). Back 3428A: DL5,000 DNA Marker
3420A	20 bp DNA Ladder (Dye Plus)	100 Rxns	USD $163.00
This marker consists of 10 double-stranded DNA fragments ranging from 20 to 200 bp in multiples of 20 bp, plus additional 300, 400, and 500 bp fragments. The 100, 200, and 500 bp fragments are brighter than the other fragments and serve as visible reference indicators; all other fragments appear with equal intensity on the gel. Since this product is premixed with loading dyes and glycerol, it is possible to apply this product to agarose gel electrophoresis directly. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 3420A: 20 bp DNA Ladder (Dye Plus)
3410A	200 bp DNA Ladder	50 ug	USD $175.00
This marker consists of ten double-stranded DNA fragments ranging from 200 bp to 2 kb in multiples of 200 bp, plus additional 2.5 kb, 3 kb and 4 kb fragments. The 1- and 2-kb fragments are brighter than the other fragments and serve as visible reference indicators; all other fragments appear with equal intensity on the gel. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 3410A: 200 bp DNA Ladder Back Migration patterns of 20 bp DNA Ladder (Cat.# 3409A), 100 bp DNA Ladder (Cat.# 3407A), 200 bp DNA Ladder (Cat.# 3410A), 500 bp DNA Ladder (Cat.# 3411A), 1 kb DNA Ladder (Cat.# 3412A), and 2.5 kb DNA Ladder (Cat.# 3413A). Migration patterns of 20 bp DNA Ladder (Cat.# 3409A), 100 bp DNA Ladder (Cat.# 3407A), 200 bp DNA Ladder (Cat.# 3410A), 500 bp DNA Ladder (Cat.# 3411A), 1 kb DNA Ladder (Cat.# 3412A), and 2.5 kb DNA Ladder (Cat.# 3413A).
3409A	20 bp DNA Ladder	50 ug	USD $175.00
This marker consists of ten double-stranded fragments ranging from 20 to 200 bp in 20-bp units, plus additional 300, 400, and 500 bp fragments. The 100, 200, and 500 bp fragments are brighter than the other fragments and serve as visible reference indicators; all other fragments appear with equal intensity on the gel. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Guidelines for selecting DNA ladders Guidelines for selecting DNA ladders. This table contains a complete listing of our DNA ladders (including wide-range DNA ladders), with catalog numbers. Each ladder is listed according to the lower size limit or by size range, with or without loading dye. The table also displays each ladder’s gel banding pattern and fragment sizes. Thicker bands are present in higher amounts and reflect the actual banding pattern on the gel. Back 3409A: 20 bp DNA Ladder Back Migration patterns of 20 bp DNA Ladder (Cat.# 3409A), 100 bp DNA Ladder (Cat.# 3407A), 200 bp DNA Ladder (Cat.# 3410A), 500 bp DNA Ladder (Cat.# 3411A), 1 kb DNA Ladder (Cat.# 3412A), and 2.5 kb DNA Ladder (Cat.# 3413A). Migration patterns of 20 bp DNA Ladder (Cat.# 3409A), 100 bp DNA Ladder (Cat.# 3407A), 200 bp DNA Ladder (Cat.# 3410A), 500 bp DNA Ladder (Cat.# 3411A), 1 kb DNA Ladder (Cat.# 3412A), and 2.5 kb DNA Ladder (Cat.# 3413A).
3407A	100 bp DNA Ladder	50 ug	USD $175.00
Takara offers a variety of DNA ladders suitable for different applications. This marker consists of 10 double-stranded DNA fragments ranging from 100 bp to 1 kb in 100-bp units, plus an additional 1.5 kb fragment. The 500-bp band contains triple the mass of the other fragments and serves as a visible reference indicator; all other fragments appear with equal intensity on the gel. They are all double-stranded DNA fragments. Loading volume is 5 μl per lane. 5 μl of this product contains ~50 ng of each band, except the 500 bp fragment which contains ~150 ng. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 3407A: 100 bp DNA Ladder Back Migration patterns of 20 bp DNA Ladder (Cat.# 3409A), 100 bp DNA Ladder (Cat.# 3407A), 200 bp DNA Ladder (Cat.# 3410A), 500 bp DNA Ladder (Cat.# 3411A), 1 kb DNA Ladder (Cat.# 3412A), and 2.5 kb DNA Ladder (Cat.# 3413A). Migration patterns of 20 bp DNA Ladder (Cat.# 3409A), 100 bp DNA Ladder (Cat.# 3407A), 200 bp DNA Ladder (Cat.# 3410A), 500 bp DNA Ladder (Cat.# 3411A), 1 kb DNA Ladder (Cat.# 3412A), and 2.5 kb DNA Ladder (Cat.# 3413A).

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Buffers

3401–3413A: 10 mM Tris-HCl (pH 8.0), 1 mM EDTA
3407A and 3407B: 10 mM Tris-HCl (pH 7.4), 1 mM EDTA
3415: 10 mM Tris-HCl (pH 8.0), 1 mM EDTA, 6% glycerol, 0.02% bromophenol blue, 0.02% xylene cyanol, 0.02% NaN₃
Supplied 6X Loading Buffer: 36% glycerol, 30 mM EDTA, 0.05% bromophenol blue, 0.035% xylene cyanol

Notes

Terminal fragments of the lambda DNA digests contain COS ends; therefore, heat treatment is required prior to use. Fragment denaturation may occur if dilution and heating are not performed in TE or TEN buffer.
Do not use these markers with polyacrylamide gels.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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DNA molecular weight markers

Notice to purchaser

Migration patterns for Wide Range DNA Ladders: Cat.# 3415A (50-10,000 bp), Cat.# 3427A (100-2,000 bp), and Cat.# 3428A (100-5,000 bp)

3428A: DL5,000 DNA Marker

Notice to purchaser

3420A: 20 bp DNA Ladder (Dye Plus)

Notice to purchaser

3410A: 200 bp DNA Ladder

Migration patterns of 20 bp DNA Ladder (Cat.# 3409A), 100 bp DNA Ladder (Cat.# 3407A), 200 bp DNA Ladder (Cat.# 3410A), 500 bp DNA Ladder (Cat.# 3411A), 1 kb DNA Ladder (Cat.# 3412A), and 2.5 kb DNA Ladder (Cat.# 3413A).

Notice to purchaser

Guidelines for selecting DNA ladders

3409A: 20 bp DNA Ladder

Migration patterns of 20 bp DNA Ladder (Cat.# 3409A), 100 bp DNA Ladder (Cat.# 3407A), 200 bp DNA Ladder (Cat.# 3410A), 500 bp DNA Ladder (Cat.# 3411A), 1 kb DNA Ladder (Cat.# 3412A), and 2.5 kb DNA Ladder (Cat.# 3413A).

Notice to purchaser

3407A: 100 bp DNA Ladder

Migration patterns of 20 bp DNA Ladder (Cat.# 3409A), 100 bp DNA Ladder (Cat.# 3407A), 200 bp DNA Ladder (Cat.# 3410A), 500 bp DNA Ladder (Cat.# 3411A), 1 kb DNA Ladder (Cat.# 3412A), and 2.5 kb DNA Ladder (Cat.# 3413A).

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Buffers

Notes

Additional product information