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RHB-A: neural stem cell culture and differentiation medium

RHB-A is a fully defined, serum-free cell culture medium optimized for the derivation, maintenance, expansion, and differentiation of human and mouse neural stem (NS) cells. RHB-A is specifically formulated for culturing pure populations of adherent human and mouse NS cells. This medium ensures the long-term stability of NS cell differentiation capacity (Sun et al. 2008) and can be used to support differentiation of human and mouse NS cells into functional neurons (Sun et al. 2008; Conti et al. 2005; Hansen et al. 2010) and of mouse ES cells into neural precursors (Ying et al. 2003; Diogo, Henrique, and Cabral 2008; Abranches et al. 2009). Critically, RHB-A medium is more efficient at neural differentiation than conventional N2- and B27-based methods. RHB-A medium can be used to support organotypic slice culture of central nervous system tissues such as brain and spinal cord, and supports the culture of glioblastoma stem cells used in cancer research.

NOTE: RHB-A medium requires supplementation with Epidermal Growth Factor (EGF) and Fibroblast Growth Factor (FGF-2) for most applications (not supplied). RHB‐Basal medium does not contain any growth factors or neuronal supplements. Therefore, this medium can be tailored to the specific requirements of your cell type by the addition of supplements.

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Cat. #	Product	Size	Price	License	Quantity	Details
Y40001	RHB-A®	500 mL	USD $369.00
RHB-A, supplemented with Epidermal Growth Factor (EGF) and Fibroblast Growth Factor-2 (FGF-2), enables the maintenance and continual expansion of symmetrically-dividing NS cells in defined, serum-free adherent culture. In growth factor supplemented RHB-A, NS cells have been demonstrated to retain their neurogenic capacity for over 100 generations, with full maintenance of diploid karyotype. RHB-A in the presence of EGF and FGF-2 also supports the derivation of clonogenic NS cell lines. Culture of adherent NS cells in RHB-A with sequential growth factor withdrawal leads to differentiation into functional neurons. RHB-A supplemented with EGF and FGF-2 has recently been used for the propagation of glioblastoma stem cell lines. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Adherent human neural stem cells differentiated in RHB-A culture medium express neural lineage markers, including microtubule associate protein 2 (Panel B), neuron-specific class III beta-tubulin (Panel C), gamma amino butyric acid (Panel D), and glial fibrillary acidic protein (Panel E) Adherent human neural stem cells differentiated in RHB-A culture medium express neural lineage markers, including microtubule associate protein 2 (Panel B), neuron-specific class III beta-tubulin (Panel C), gamma amino butyric acid (Panel D), and glial fibrillary acidic protein (Panel E). A phase contrast image (Panel A) and a merge of neuron-specific class III beta-tubulin and glial fibrillary acidic protein images (F) are also shown. Back Adherent human neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express neural stem cell markers Adherent human neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express neural stem cell markers. Expression of nestin (Panel A), vimentin (Panel B), 3CB2 (Panel C), and microtubule associated protein 2 (Panel D) were assessed by immunofluorescence. Back Adherent mouse neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express sex determining region Y-box 2 (Sox2, red) and Nestin (green) Adherent mouse neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express sex determining region Y-box 2 (Sox2, red) and Nestin (green). Back Adherent mouse neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express neural stem cell markers, including Nestin, Vimentin (3CB2), radial glial cell marker-2 (RC2), glial fibrillary acidic protein (GFAP), and microtubule associate protein (MAP) Adherent mouse neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express neural stem cell markers, including Nestin, Vimentin (3CB2), radial glial cell marker-2 (RC2), glial fibrillary acidic protein (GFAP), and microtubule associate protein (MAP). Back Human neural cortex stem cell lines were differentiated into either neurons (MAP2, red) or astrocytes (GFAP, green) using RHB-A Human neural cortex stem cell lines were differentiated into either neurons (MAP2, red) or astrocytes (GFAP, green) using RHB-A. Back Fold expansion profiles of human neural stem cell lines cultured by different vendor’s systems Fold expansion profiles of human neural stem cell lines cultured by different vendor’s systems. RHB-A exhibits robust growth and expansion of human neural stem cells. Back Y40001: RHB-A

Overview

RHB-A is a proprietary, fully defined, and serum-free medium designed to maintain pure populations of adherent human and mouse NS cells
RHB-Basal medium is free of animal components and neuronal supplements; the medium can be customized by the addition of supplements

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Applications

Derivation of mouse and human NS cells from ES cells and fetal and adult tissues
Maintenance and propagation of adherent mouse and human NS cells
Differentiation of mouse and human NS cells into functional neurons
Differentiation of mouse ES cells to neuronal precursors
Organotypic slice culture of CNS tissue
Glioblastoma stem cell culture for cancer research
Refer to the Data Sheet for additional examples of use

Components

500 ml medium

References

Abranches, E. et al. Neural Differentiation of Embryonic Stem Cells In Vitro: A Road Map to Neurogenesis in the Embryo. PLoS One 4, e6286 (2009).

Conti, L. et al. Niche-Independent Symmetrical Self-Renewal of a Mammalian Tissue Stem Cell. PLoS Biol. 3, e283 (2005).

Diogo, M. M., Henrique, D. & Cabral, J. M. S. Optimization and integration of expansion and neural commitment of mouse embryonic stem cells. Biotechnol. Appl. Biochem. 49, 105 (2008).

Hansen, D. V., Lui, J. H., Parker, P. R. L. & Kriegstein, A. R. Neurogenic radial glia in the outer subventricular zone of human neocortex. Nature 464, 554–561 (2010).

Sun, Y. et al. Long-term tripotent differentiation capacity of human neural stem (NS) cells in adherent culture. Mol. Cell. Neurosci. 38, 245–258 (2008).

Ying, Q. L., Stavridis, M., Griffiths, D., Li, M. & Smith, A. Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nat. Biotechnol. 21, 183–186 (2003).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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RHB-A: neural stem cell culture and differentiation medium

Notice to purchaser

Adherent human neural stem cells differentiated in RHB-A culture medium express neural lineage markers, including microtubule associate protein 2 (Panel B), neuron-specific class III beta-tubulin (Panel C), gamma amino butyric acid (Panel D), and glial fibrillary acidic protein (Panel E)

Adherent human neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express neural stem cell markers

Adherent mouse neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express sex determining region Y-box 2 (Sox2, red) and Nestin (green)

Human neural cortex stem cell lines were differentiated into either neurons (MAP2, red) or astrocytes (GFAP, green) using RHB-A

Fold expansion profiles of human neural stem cell lines cultured by different vendor’s systems