NDiff 227 is a proprietary, defined, serum-free medium for the neural differentiation of mouse ES cells in adherent monolayer culture conditions. NDiff 227 has also been shown to support the serum-free, feeder-free culture of mouse ES cells. More recently, 227-like medium supplemented with growth factors has also been used to successfully maintain feeder-free human ES cells in culture. When supplemented with FGF, serum-free 227 also generates Anterior Definitive Endoderm (ADE) precursors from mouse ES cells, which can then be differentiated into liver and pancreas with enhanced efficiency.