Protein ladder sets: CLEARLY Protein Ladder molecular weight markers

Convenient and ready-to-use CLEARLY Protein Ladder sets are mixtures of 11 highly purified recombinant proteins. When used during SDS-PAGE, these protein ladder sets provide molecular weight markers ranging from 10–250 kDa (unstained ladder) or 10–210 kDa (pre-stained ladder). Load directly on gels—no pre-heating is necessary. CLEARLY Protein Ladder (Unstained) is recommended as a precise molecular weight standard. Use the CLEARLY Stained Protein Ladder to easily monitor the efficiency of protein transfer to a Western blot. Because the conjugation of dye to proteins in the CLEARLY Stained Protein Ladder affects migration of the highest molecular weight proteins in the ladder, it is not recommended as a reference for precise molecular weight calculations. In this instance, use the unstained ladder.

Cat. #	Product	Size	Quantity
3453A	CLEARLY Protein Ladder (Unstained)	500 uL	*
CLEARLY Protein Ladder (Unstained) is a mixture of 11 recombinant highly purified proteins. When this ladder is analyzed by SDS-PAGE and stained with Coomassie Brilliant Blue R-250, it is resolved into quite clearly identifiable bands from 10 to 250 kDa. This ladder is intended for use as a precise size standard when performing SDS-PAGE to calculate the molecular weight of a protein of interest. The 30 and 80 kDa bands have quadruple the mass of the other bands and serve as reference indicators. This ladder is pre-mixed with loading buffer and ready to load. Preheating is not required. 5 μl of this ladder is loaded per lane of SDS-PAGE minigel for standard use. In this case, this ladder is sufficient for 100 lanes. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Migration pattern of CLEARLY Protein Ladder (Unstained) (Cat.# 3453A) on a 12% SDS-PAGE gel. Migration pattern of CLEARLY Protein Ladder (Unstained) (Cat.# 3453A) on a 12% SDS-PAGE gel.
3453B	CLEARLY Protein Ladder (Unstained)	1,500 uL	*
CLEARLY Protein Ladder (Unstained) is a mixture of 11 recombinant highly purified proteins. When this ladder is analyzed by SDS-PAGE and stained with Coomassie Brilliant Blue R-250, it is resolved into quite clearly identifiable bands from 10 to 250 kDa. This ladder is intended for use as a precise size standard when performing SDS-PAGE to calculate the molecular weight of a protein of interest. The 30 and 80 kDa bands have quadruple the mass of the other bands and serve as reference indicators. This ladder is pre-mixed with loading buffer and ready to load. Pre-heating is not required. 5 μl of this ladder is loaded per lane of SDS-PAGE minigel for standard use. In this case, this ladder is sufficient for 100 lanes. Cat. # 3453B contains 3 of Cat. # 3453A. Please refer to Cat. # 3453A for complete product documentation and resources. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents
3454A	CLEARLY Stained Protein Ladder	500 uL	*
CLEARLY Stained Protein Ladder is a mixture of 11 pre-stained recombinant highly purified proteins. When this ladder is analyzed by SDS-PAGE, it is resolved into clearly identifiable visible bands from 10 to 210 kDa. The 30 and 70 kDa bands have quadruple the mass of the other bands and serve as reference indicators. This ladder is pre-mixed with loading buffer and ready to load. Pre-heating is not required. 10 μl of this ladder is loaded per lane of SDS-PAGE minigel for standard use. In this case, this ladder is sufficient for 50 lanes. This ladder is useful for monitoring of protein migration during SDS-PAGE and verifying transfer efficiency at western blotting.^＊＊: Because the covalent coupling of the dye to the proteins affects their electrophoretic behavior on SDS-PAGE gels relative to unstained proteins, this ladder is not recommended for use as a precise size standard when performing SDS-PAGE to calculate the molecular weight of a protein of interest. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Migration pattern of CLEARLY Stained Protein Ladder (Cat.# 3454A) on an 12% SDS-PAGE gel Migration pattern of CLEARLY Stained Protein Ladder (Cat.# 3454A) on an 12% SDS-PAGE gel.
3454B	CLEARLY Stained Protein Ladder	1,500 uL	*
CLEARLY Stained Protein Ladder is a mixture of 11 pre-stained recombinant highly purified proteins. When this ladder is analyzed by SDS-PAGE, it is resolved into clearly identifiable visible bands from 10 to 210 kDa. The 30 and 70 kDa bands have quadruple the mass of the other bands and serve as reference indicators. This ladder is pre-mixed with loading buffer and ready to load. Pre-heating is not required. 10 μl of this ladder is loaded per lane of SDS-PAGE minigel for standard use. In this case, this ladder is sufficient for 50 lanes. This ladder is useful for monitoring of protein migration during SDS-PAGE and verifying transfer efficiency at western blotting.^＊＊: Because the covalent coupling of the dye to the proteins affects their electrophoretic behavior on SDS-PAGE gels relative to unstained proteins, this ladder is not recommended for use as a precise size standard when performing SDS-PAGE to calculate the molecular weight of a protein of interest. Cat. # 3454B contains 3 of Cat. # 3454A. Please refer to Cat. # 3454A for complete product documentation and resources. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents

More Information

Protein ladder sets

CLEARLY Protein Ladder (Unstained) (Cat.# 3453A and 3453B)

Molecular weight

250 kDa

150 kDa

100 kDa

80 kDa*

60 kDa

50 kDa

40 kDa

30 kDa*

20 kDa

15 kDa

10 kDa

*Reference band (four-fold greater intensity relative to the other bands)

CLEARLY Stained Protein Ladder (Cat.# 3454A and 3454B)

Apparent molecular weight

210 kDa

140 kDa

100 kDa

70 kDa*

55 kDa

45 kDa

35 kDa

30 kDa*

20 kDa

15 kDa

10 kDa

*Reference band (four-fold greater intensity relative to the other bands)

SDS-PAGE recommendations

5–20% gradient gels are recommended.
Unstained ladder (Cat.# 3453): Load 5 µl per lane.
Pre-stained ladder (Cat.# 3454): Load 10 µl per lane.
Ensure that the ladder is completely thawed before removing an aliquot for loading.

Storage

–20°C

These products are stable for 6 months when stored at 4°C. They remain stable for up to 50 freeze-thaw cycles.

Quantity

CLEARLY Protein Ladder (Unstained) is sufficient for 100 lanes. CLEARLY Stained Protein Ladder is sufficient for 50 lanes.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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SDS-PAGE & western blotting

Protein ladder sets: CLEARLY Protein Ladder molecular weight markers

Notice to purchaser

Migration pattern of CLEARLY Protein Ladder (Unstained) (Cat.# 3453A) on a 12% SDS-PAGE gel.

Notice to purchaser

Notice to purchaser

Migration pattern of CLEARLY Stained Protein Ladder (Cat.# 3454A) on an 12% SDS-PAGE gel

Notice to purchaser

More Information

Protein ladder sets

SDS-PAGE recommendations

Storage

Quantity

Additional product information