Methylation-specific PCR of bisulfite-treated DNA templates: TaKaRa EpiTaq HS (for bisulfite-treated DNA)
DNA methylation analysis often incorporates bisulfite treatment of DNA in order to determine the methylation status of specific CpG regions. EpiTaq HS (for bisulfite-treated DNA) is a hot-start (HS) DNA polymerase that is optimized for PCR amplification of bisulfite-treated template DNA. PCR amplification of bisulfite-treated DNA often poses technical challenges for conventional polymerases due to the presence of uracils in the treated DNA. However, EpiTaq HS polymerase and other kit reagents allow for the adjustment of magnesium and dNTP concentrations to achieve optimal amplification efficiency and specificity, enabling the amplification of challenging targets.
EpiTaq HS is a hot-start PCR enzyme coupled with an anti-Taq antibody. Prior to high-temperature denaturation, the anti-Taq antibody suppresses polymerase activity, thereby preventing non-specific amplification as a result of mispriming or primer-dimer formation prior to PCR. The presence of the anti-Taq antibody allows for conventional PCR setup with no additional denaturation step.
- Superior amplification of bisulfite-treated DNA
- Efficient amplification of a range of amplicons ranging from short (150 bp) to long (1,000 bp)
- EpiTaq HS enzyme inhibits non-specific amplification and primer-dimer formation
- Amplified product can be used directly for TA cloning applications
- Methylation analysis of CpG Islands
- Amplification of bisulfite-treated DNA, including DNA templates that contain uracils
- DNA amplification of AT/GC rich templates
|(200 reactions x 50 µl PCR)|
|EpiTaq HS （5 U/μl)||250 U|
|10X EpiTaq PCR Buffer (Mg2+ free)||1 ml|
|dNTP Mixture （2.5 mM each dNTP）||1.2 ml|
|25 mM MgCl2||1.2 ml|
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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