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Total RNA sequencing of liquid biopsies
RNA profiling has emerged as a powerful tool to investigate the biomarker potential of human biofluids. However, despite enormous interest in extracellular nucleic acids, RNA sequencing methods to quantify the total RNA content outside cells are rare.
In this webinar, Jo Vandesompele, PhD and chief scientific officer at Biogazelle, will discuss the performance of the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian with various human biofluids and extracellular vesicles (EVs) purified from these biofluids.
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- Application of SMARTer stranded chemistry to various biofluids and extracellular vesicles
- Accuracy, precision, and sensitivity of the method with low-input volumes
- Ability to quantify thousands of genes, allowing the detection of a diversity of RNA biotypes and their heterogeneity among different biofluids
- Performance data on other sample types, including FFPE tissue and single cells
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