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Taking single-cell RNA-seq by STORM: nanoliter-scale, high-resolution, total single-cell transcriptome profiling in primary cryopreserved tissue
Scientists are moving towards miniaturization of their NGS workflows as a way to lower costs, but equally important is maintaining the quality of the data when compared to full-volume workflows.
Experts from the research and core facilities at the Van Andel Institute used one such approach to miniaturize the SMART-Seq Stranded Kit on the SPT Labtech mosquito HV instrument and develop a new method. In this webinar, cohosted by Takara Bio and SPT, you'll learn more about STORM-seq (Single-cell TOtal RNA Miniaturized sequencing) and hear how the SMART-Seq Stranded Kit was miniaturized to yield robust single-cell total RNA libraries, the challenges the researchers faced, and how they overcame these challenges.
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Learn more about their experiences:
- Miniaturizing the SMART-Seq Stranded Kit on the mosquito HV
- Overcoming the hurdles they met while adapting a library prep method to an automated miniaturized platform
- Performing in-depth single-cell profiling of specific tissues
Genomics Core Associate II
Ben Johnson, PhD
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Learn more about our single-cell RNA-seq technologies:
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