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- Takara Bio Single-Cell Workshop, Spring 2021
- Sensitivity and scale for neuron multiomics
- Liver metabolic function, dissecting one cell at a time
- Pushing the limits of sensitivity for single-cell applications
- Single-Cell Workshop at 2020 NextGen Omics Series UK
- TCR-seq methods: when to use which
- Taking single-cell RNA-seq by STORM
- Immunogenomics to accelerate immunotherapy
- Total RNA sequencing of liquid biopsies
- MeD-Seq, a novel method to detect DNA methylation
- Automating full-length single-cell RNA-seq libraries
- Single-cell DNA-seq
- Single-cell whole transcriptome analysis
Automating full-length single-cell RNA-seq libraries
Interest in understanding the human genome at the single-cell level, in order to further understand cellular dynamics, has grown worldwide, as indicated by the increase in consortia devoted to this field. To aid in this research, both core facilities and automation play key roles. Core facilities provide researchers cost-effective and efficient access to new methods, while automation provides reliable, efficient, and cost-effective processing of high-throughput samples.
In this on-demand webinar, Dr. Jürgen Zimmermann, a senior engineer at the EMBL Genomics Core Facility, highlights important considerations for automation and discusses how he has optimized the Beckman Coulter Biomek for automating the SMART-Seq HT Kit for up to 192 samples, and counting. The SMART-Seq HT Kit uses oligo(dT)-priming to generate high-quality, full-length cDNA directly from 1–100 cells with a streamlined protocol.
Presented in partnership with Beckman Coulter (a Danaher company).
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