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  • Pushing the limits of sensitivity for single-cell applications
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  • Automating full-length single-cell RNA-seq libraries
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Home › Learning centers › Next-generation sequencing › RNA-seq › Webinars › Automating full-length single-cell RNA-seq libraries

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    • Taking single-cell RNA-seq by STORM
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    • Liver metabolic function, dissecting one cell at a time
    • Pushing the limits Q&A
    • Total RNA sequencing of liquid biopsies
    • Liver metabolic function Q&A
    • Automating full-length single-cell RNA-seq libraries
    • Single-cell whole transcriptome analysis
    • Sensitivity and scale for neuron multiomics
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Automating full-length single-cell RNA-seq libraries

Interest in understanding the human genome at the single-cell level, in order to further understand cellular dynamics, has grown worldwide, as indicated by the increase in consortia devoted to this field. To aid in this research, both core facilities and automation play key roles. Core facilities provide researchers cost-effective and efficient access to new methods, while automation provides reliable, efficient, and cost-effective processing of high-throughput samples.

In this on-demand webinar, Dr. Jürgen Zimmermann, a senior engineer at the EMBL Genomics Core Facility, highlights important considerations for automation and discusses how he has optimized the Beckman Coulter Biomek for automating the SMART-Seq HT Kit for up to 192 samples, and counting. The SMART-Seq HT Kit uses oligo(dT)-priming to generate high-quality, full-length cDNA directly from 1–100 cells with a streamlined protocol.

Presented in partnership with Beckman Coulter (a Danaher company).

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
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  • CRISPR screening
  • Drug discovery
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  • Next-generation sequencing
  • Spatial omics
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  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Bioinformatics tools
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  • Real-time PCR
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  • Signature enzymes
  • High-throughput real-time PCR solutions
  • Detection assays
  • References, standards, and buffers
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  • Media, differentiation kits, and matrices
  • Stem cells and stem cell-derived cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
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  • Competent cells
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