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  • ‹ Back to RNA-seq
  • Stranded libraries from picogram-input total RNA (v3)
  • Automation-friendly, all-in-one cDNA synthesis and library prep
  • All-in-one cDNA synthesis and library prep from ultra-low RNA inputs
  • Stranded libraries from picogram-input total RNA (v2)
  • Stranded libraries from FFPE inputs (v2)
  • Stranded libraries from 100 ng - 1 ug total RNA
  • Stranded libraries from 100 pg-100 ng total RNA
  • Stranded libraries from picogram-input total RNA (v1)
  • Stranded RNA-seq competitor kit comparison
  • Nonstranded libraries from FFPE inputs
  • Sensitive capture of full-length transcript information with targeted RNA-seq
Low-input SMARTer stranded RNA-seq kits
Home › Learning centers › Next-generation sequencing › Technical notes › RNA-seq › Stranded libraries from 100 pg-100 ng total RNA

Technical notes

  • Single-cell RNA- and DNA-seq
    • All-in-one cDNA synthesis and library prep from single cells
    • Highest sensitivity for single-cell mRNA-seq
    • Stranded libraries from single cells
    • Streamlined single-cell mRNA-seq
    • Full-length mRNA libraries from single cells (SMART-Seq v4)
    • 3' mRNA libraries from single cells (SMART-Seq v4 3' DE Kit)
    • Full-length mRNA libraries from single cells for Fluidigm C1 (SMART-Seq v4)
    • Full-length single-cell library method comparison
    • High-resolution CNV detection using PicoPLEX Gold DNA-Seq
    • Next-gen WGA method for CNV and SNV detection from single cells
  • RNA-seq
    • Stranded libraries from picogram-input total RNA (v3)
    • Automation-friendly, all-in-one cDNA synthesis and library prep
    • All-in-one cDNA synthesis and library prep from ultra-low RNA inputs
    • Stranded libraries from picogram-input total RNA (v2)
    • Stranded libraries from FFPE inputs (v2)
    • Stranded libraries from 100 ng - 1 ug total RNA
    • Stranded libraries from 100 pg-100 ng total RNA
    • Stranded libraries from picogram-input total RNA (v1)
    • Stranded RNA-seq competitor kit comparison
    • Nonstranded libraries from FFPE inputs
    • Sensitive capture of full-length transcript information with targeted RNA-seq
  • DNA-seq
    • Comparing ThruPLEX HV PLUS to Kapa and NEBNext
    • Improvements to ThruPLEX HV
    • ThruPLEX HV outperforms NEBNext Ultra II
    • Accurate detection of low-frequency variants using molecular tags
    • Streamlined DNA-seq from challenging samples
    • Low cell number ChIP-seq using SMARTer ThruPLEX
    • Cell-free DNA sequencing
    • Sequencing analysis of low-frequency mutations in cfDNA
    • DNA-seq from FFPE samples
    • Low-input whole-exome sequencing
    • Tag-seq variant detection
    • Low-volume DNA shearing for SMARTer ThruPLEX library prep
  • Immune profiling
    • BCR repertoire profiling from human samples (bulk)
    • Improved TCR repertoire profiling from human samples (bulk)
    • TCR repertoire profiling from human samples (single cells)
    • TCR repertoire profiling from human samples (bulk)
    • TCR repertoire profiling from mouse samples (bulk)
    • BCR repertoire profiling from mouse samples (bulk)
  • Epigenetics and smRNA-seq
    • Full-length small RNA libraries
    • ChIP-seq libraries for transcription factor analysis
    • ChIP-seq libraries from ssDNA
    • Methylated DNA-seq
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Low-input SMARTer stranded RNA-seq kits
Tech Note

A complete kit for stranded RNA-seq library preparation

  • Stranded information:
    Accurate identification of transcript strand of origin
  • Ribosomal RNA depletion:
    Efficient rRNA removal in a complete sample preparation kit
  • Integrated library preparation:
    High-quality sequencing data generated on Illumina platforms
Introduction Results Conclusions Methods References

Introduction  

RNA sequencing (RNA-seq) is a key tool for performing expression analysis of the entire transcriptome with high sensitivity and a wide dynamic range. Random-primed cDNA synthesis kits, like the SMARTer Stranded RNA-seq kits, are ideal for transcriptome analysis from all types of input RNA, including compromised samples. These kits are based on SMART (Switching Mechanism at 5' End of RNA Template) technology, which is an inherently strand-specific reverse transcription reaction leading to ≥99% accurate identification of the strand of origin without the need for additional preparation steps. Illumina adapters (up to 96 different indexes) are added during cDNA amplification eliminating further library preparation steps after cDNA synthesis.

Prior to cDNA synthesis with any random-primed cDNA synthesis kit, it is important to remove ribosomal RNA (rRNA), which can represent up to 90% of total RNA. The RiboGone - Mammalian kit uses hybridization technology and RNase H digestion to bind and specifically deplete 5S, 5.8S, 18S, and 28S nuclear rRNA sequences and 12S mitochondrial RNA (mtRNA) sequences from full-length or sheared total RNA derived from human, mouse, or rat samples. (This kit does not deplete 16S mitochondrial RNA sequences, which share significant homology with some nuclear genes). The SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian, combines these two technologies into one convenient kit for complete sample preparation and cDNA synthesis.

Results  

Advantages of strand-of-origin information

Maintaining strand-of-origin information in cDNA libraries for sequencing allows researchers to identify overlapping transcripts, which are common in compact bacterial genomes, and antisense transcripts that will be lost with a strand-agnostic cDNA synthesis method. Using the SMARTer stranded RNA-seq kits, we have been able to identify both overlapping and antisense transcripts correctly.

SMARTer Stranded kits distinguish overlapping and antisense transcripts

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-seq Kit. Panel A. RNA-seq reads from a Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen et al. 2011). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method.

RiboGone - Mammalian efficiently removes rRNA sequences from total RNA

Both RiboGone-treated and oligo(dT)-purified RNA sample inputs generated RNA-seq data with similarly low percent sequencing reads mapping to rRNA. Both intact (Human Brain Total RNA) and degraded (FFPE tissue) RNA samples are suitable for the SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian. Oligo(dT)-based methods for decreasing the number of rRNA reads also decrease the number of sequencing reads mapping to noncoding RNAs. The RiboGone method is based on selective hybridization to rRNA leaving both mRNA and noncoding RNAs available as templates for the reverse transcription reaction.

RiboGone - Mammalian efficiently removed rRNA from intact and degraded RNA samples

Efficient rRNA removal with the RiboGone - Mammalian kit. RNA-seq libraries were generated from Human Brain Total RNA or Breast Cancer FFPE RNA using the SMARTer Stranded RNA-seq Kit. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA while retaining more noncoding reads.

High-quality sequencing data

The SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian maintains the ability of other SMARTer Stranded kits to generate RNA-seq libraries from a variety of samples including Human Universal Reference RNA (HURR; Agilent) and Human Brain Reference RNA (HBRR; Ambion). When libraries were sequenced on an Illumina MiSeq® instrument, both the HURR and HBRR samples yielded a high number of reads, with 75–76% mapped, 66–70% uniquely mapped, over 13,800 genes identified, and less than 1% of reads mapped to rRNA.

Sequence alignment metrics
 Human Universal Reference RNA (HURR)Human Brain Reference RNA (HBRR)
No. of reads 6,829,540 7,728,850
Mapped to rRNA 62,792 (0.9%) 49,844 (0.7%)
Mapped to mitochondrial RNA 318,006 (4.7%) 224,939 (2.9%)
Mapped to RefSeq 4,871,900 (76%) 5,515,264 (75%)
Mapped uniquely to RefSeq 4,435,123 (70%) 4,888,340 (66%)
Exons 2,311,575 (47%) 2,712,444 (49%)
Introns 2,560,325 (53%) 2,802,820 (51%)
Genes identified 14,563 13,839

Sequencing alignment metrics for HURR and HBRR libraries. 10 ng samples of intact HURR and HBRR were used as input for the SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian. RNA-seq libraries were prepared according to the kit protocol and sequenced on an Illumina MiSeq platform. RNA-seq data obtained with the SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian for HURR and HBRR samples correlate with qPCR data for the same RNAs obtained through the MicroArray Quality Control (MAQC) study (Shi et al. 2006). The high level of correlation with MAQC (R = 0.860) suggests that the RNA-seq data was not affected by rRNA depletion with the RiboGone - Mammalian kit.

RNA-seq and MAQC correlation: RiboGone does not interfere with sequencing

High correlation between SMARTer Stranded RNA-seq data and MAQC qPCR data. A scatter plot was used to compare differential expression data obtained from SMARTer transcriptome analysis of HURR and HBRR cDNA libraries (in Reads per Kilobase of Exon per Million Reads; RPKM) and qPCR data for HURR and HBRR (in Ct) from the MAQC project. The transcripts used in this analysis were the 623 of ~900 transcripts present in the MAQC data set that were also detected in both the HURR and HBRR SMARTer stranded RNA-seq data sets.

Conclusions  

The SMARTer stranded RNA-seq kits generate RNA-seq libraries from intact or degraded RNA samples that retain the strand-of-origin information. Strand-of-origin information can be used to identify overlapping and antisense transcripts. Sequencing data generated with these kits identify a large number of genes that highly correlate with MAQC data. The SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian combines the efficient rRNA-removal of RiboGone - Mammalian with cDNA synthesis. The data generated with this complete kit maintains the high quality of other SMARTer stranded RNA-seq kits.

Methods  

Human Brain Poly A+ RNA was spiked with ERCC control RNA and serially diluted to prepare RNA samples containing between 100 pg–100 ng RNA. cDNA libraries were prepared using the SMARTer Stranded RNA-seq Kit according to the kit protocol with twelve different Illumina indices. Libraries were sequenced on an Illumina HiSeq® 2000 instrument, with ~300M 2 x 100 bp paired-end reads.

RNA was generated from Breast Cancer FFPE RNA (Cureline) using a NucleoSpin totalRNA FFPE kit. This RNA and Human Brain Total RNA was treated with either the RiboGone - Mammalian kit or the Magnosphere UltraPure mRNA Purification Kit, according to the specific kit protocol. Untreated total RNA was also used as input for RNA-seq library production in order to identify the high percent of rRNA reads present in the initial total RNA preparations. RNA-seq libraries were generated with the SMARTer Stranded RNA-seq Kit and sequenced on an Illumina MiSeq instrument. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-seq Metrics.

RNA-seq libraries were generated from 10 ng samples of Human Universal Reference RNA (Agilent) and Human Brain Reference RNA (Ambion), the same RNAs used in the MAQC project (Shi et al. 2006), using the SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian according to the kit protocol, using 18 cycles of PCR. Libraries were sequenced on an Illumina MiSeq platform with ~7M 1 x 50 bp single-end reads per library.

Reads were trimmed by CLC Genomics Workbench and mapped to rRNA and the mitochondrial genome with CLC (% reads indicated). The unmapped reads were subsequently mapped with CLC to the human genome with RefSeq masking, producing mapped reads and uniquely mapped reads. The number of genes identified in each library was determined by the number of genes with an RPKM of at least 0.1. The number of reads that map to introns or exons is a percentage of the reads successfully mapped to RefSeq.

References  

Hansen, T. B. et al. miRNA-dependent gene silencing involving Ago2-mediated cleavage of a circular antisense RNA. Embo J 30, 4414–4422 (2011).     

Shi, L. et al. The MicroArray Quality Control (MAQC) project shows inter-and intraplatform reproducibility of gene expression measurements. Nat. Biotechnol. 24, 1151–1161 (2006).

Related Products

Cat. # Product Size Price License Quantity Details
634846 RiboGone™ - Mammalian 6 Rxns USD $360.00

License Statement

ID Number  
246 This product is protected by U.S. Patent 9,428,794, and additional pending US and foreign patents. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

RiboGone - Mammalian allows for the specific removal of rRNA sequences (5S, 5.8S, 18S, and 28S), as well as mitochondrial rRNA sequences (12S), from human, mouse, or rat total RNA. This kit is designed for use with limited sample amounts (10–100 ng of total RNA), and works with high- or low-quality RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with random primers.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.

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Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA

Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA
Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA. The high level of correlation between the RNA-Seq and MAQC data (R=0.860) shows that depletion of rRNA with RiboGone does not interfere with detection of other genes by RNA-seq.

Back

Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples

Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples
Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples.

Back

RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA

RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA
RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with any random-primed SMARTer RNA-Seq kit (including the SMARTer Stranded RNA-Seq Kit, the SMARTer Universal Low Input RNA Kit for Sequencing, and the SMARTer Universal Low Input RNA Library Prep Kit).

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634846: RiboGone - Mammalian

634846: RiboGone - Mammalian

Required Products

Cat. # Product Size Price License Quantity Details
634836 SMARTer® Stranded RNA-Seq Kit 12 Rxns USD $1027.00

The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis).

The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA.

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Back

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations
cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit).

Back

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels
The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain polyA+ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations
Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments
Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments.

Back

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis
High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R2 of 0.9725. Axes are plotted on a log2 scale.

Back

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads
Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina® HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand.

Back

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit
Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method.

Back

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.
634837 SMARTer® Stranded RNA-Seq Kit 24 Rxns USD $1850.00

The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis).

The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA.

Documents Components You May Also Like Image Data Resources

Back

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations
cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit).

Back

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels
The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain polyA+ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations
Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments
Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments.

Back

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis
High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R2 of 0.9725. Axes are plotted on a log2 scale.

Back

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads
Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina® HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand.

Back

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit
Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method.

Back

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.
634838 SMARTer® Stranded RNA-Seq Kit 48 Rxns USD $3125.00

The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis).

The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA.

Documents Components You May Also Like Image Data Resources

Back

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations
cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit).

Back

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels
The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain polyA+ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations
Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments
Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments.

Back

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis
High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R2 of 0.9725. Axes are plotted on a log2 scale.

Back

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads
Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina® HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand.

Back

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit
Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method.

Back

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.
634839 SMARTer® Stranded RNA-Seq Kit 96 Rxns USD $4024.00

The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis).

The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA.

Documents Components You May Also Like Image Data Resources

Back

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations
cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit).

Back

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels
The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain polyA+ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations
Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments
Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments.

Back

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis
High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R2 of 0.9725. Axes are plotted on a log2 scale.

Back

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads
Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina® HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand.

Back

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit
Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method.

Back

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.
634847 RiboGone™ - Mammalian 24 Rxns USD $1260.00

License Statement

ID Number  
246 This product is protected by U.S. Patent 9,428,794, and additional pending US and foreign patents. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

RiboGone - Mammalian allows for the specific removal of rRNA sequences (5S, 5.8S, 18S, and 28S), as well as mitochondrial rRNA sequences (12S), from human, mouse, or rat total RNA. This kit is designed for use with limited sample amounts (10–100 ng of total RNA), and works with high- or low-quality RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with random primers.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.

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Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA

Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA
Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA. The high level of correlation between the RNA-Seq and MAQC data (R=0.860) shows that depletion of rRNA with RiboGone does not interfere with detection of other genes by RNA-seq.

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Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples

Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples
Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples.

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RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA

RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA
RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with any random-primed SMARTer RNA-Seq kit (including the SMARTer Stranded RNA-Seq Kit, the SMARTer Universal Low Input RNA Kit for Sequencing, and the SMARTer Universal Low Input RNA Library Prep Kit).

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634847: RiboGone - Mammalian

634847: RiboGone - Mammalian

Required Products

Cat. # Product Size Price License Quantity Details
634836 SMARTer® Stranded RNA-Seq Kit 12 Rxns USD $1027.00

The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis).

The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA.

Documents Components You May Also Like Image Data Resources

Back

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations
cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit).

Back

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels
The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain polyA+ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations
Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments
Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments.

Back

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis
High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R2 of 0.9725. Axes are plotted on a log2 scale.

Back

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads
Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina® HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand.

Back

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit
Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method.

Back

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.
634837 SMARTer® Stranded RNA-Seq Kit 24 Rxns USD $1850.00

The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis).

The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA.

Documents Components You May Also Like Image Data Resources

Back

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations
cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit).

Back

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels
The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain polyA+ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations
Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments
Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments.

Back

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis
High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R2 of 0.9725. Axes are plotted on a log2 scale.

Back

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads
Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina® HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand.

Back

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit
Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method.

Back

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.
634838 SMARTer® Stranded RNA-Seq Kit 48 Rxns USD $3125.00

The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis).

The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA.

Documents Components You May Also Like Image Data Resources

Back

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations
cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit).

Back

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels
The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain polyA+ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations
Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments
Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments.

Back

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis
High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R2 of 0.9725. Axes are plotted on a log2 scale.

Back

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads
Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina® HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand.

Back

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit
Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method.

Back

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.
634839 SMARTer® Stranded RNA-Seq Kit 96 Rxns USD $4024.00

The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis).

The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA.

Documents Components You May Also Like Image Data Resources

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cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations
cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit).

Back

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels
The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain polyA+ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations
Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments
Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments.

Back

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis
High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R2 of 0.9725. Axes are plotted on a log2 scale.

Back

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads
Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina® HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand.

Back

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit
Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method.

Back

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.
634836 SMARTer® Stranded RNA-Seq Kit 12 Rxns USD $1027.00

The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis).

The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources

Back

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations
cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit).

Back

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels
The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain polyA+ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations
Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments
Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments.

Back

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis
High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R2 of 0.9725. Axes are plotted on a log2 scale.

Back

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads
Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina® HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand.

Back

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit
Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method.

Back

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.
634837 SMARTer® Stranded RNA-Seq Kit 24 Rxns USD $1850.00

The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis).

The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources

Back

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations
cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit).

Back

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels
The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain polyA+ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations
Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments
Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments.

Back

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis
High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R2 of 0.9725. Axes are plotted on a log2 scale.

Back

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads
Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina® HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand.

Back

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit
Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method.

Back

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.
634838 SMARTer® Stranded RNA-Seq Kit 48 Rxns USD $3125.00

The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis).

The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources

Back

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations
cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit).

Back

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels
The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain polyA+ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations
Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments
Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments.

Back

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis
High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R2 of 0.9725. Axes are plotted on a log2 scale.

Back

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads
Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina® HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand.

Back

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit
Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method.

Back

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.
634839 SMARTer® Stranded RNA-Seq Kit 96 Rxns USD $4024.00

The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis).

The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources

Back

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations
cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit).

Back

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels
The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain polyA+ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations
Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments
Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments.

Back

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis
High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R2 of 0.9725. Axes are plotted on a log2 scale.

Back

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads
Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina® HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand.

Back

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit
Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method.

Back

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.
634862 SMARTer® Stranded RNA-Seq Kit HT 96 Rxns USD $4109.00

The SMARTer Stranded RNA-Seq Kit HT includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform in a high-throughput manner. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the Indexing Primer Set HT for Illumina (which contains 8 indexed forward PCR primers and 12 indexed reverse PCR primers for the high-throughput amplification of 96 uniquely-indexed RNA-seq libraries). This kit generates indexed, paired-end, Illumina-compatible sequencing libraries in a 96-well plate format, which enables high levels of multiplexing of NGS library analysis.

The SMARTer Stranded RNA-Seq Kit HT utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic cleanup or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA. The Indexing Primer Set HT for Illumina integrated into this kit makes it convenient to generate 96 uniquely-indexed libraries for Illumina sequencing.

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634861 SMARTer® Stranded Total RNA Sample Prep Kit - Low Input Mammalian 24 Rxns USD $2895.00

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246 This product is protected by U.S. Patent 9,428,794, and additional pending US and foreign patents. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

The SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian provides a complete solution for sensitive and streamlined total RNA analysis. The kit is designed to work with a wide range of input amounts (10–100 ng) of total RNA of any quality. A variety of human, rat, and mouse samples can be used, including total RNA from FFPE material, tissue samples, etc. The kit provides whole transcriptome analysis with accurate measurement of strand orientation, unbiased coverage, high transcript mapping, and gene counts.

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Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit
Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method.

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RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.

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